A5062877135- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Aluminum toxicity and tolerance in plants and animals
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- MicroRNA in disease regulation
- Nuclear Structure and Function
- Algal biology and biofuel production
- Antibiotic Resistance in Bacteria
- Plant Molecular Biology Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Agricultural Science and Fertilization
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
University of Liège
2010-2025
During the cell cycle of rod-shaped bacteria, two morphogenetic processes can be discriminated: length growth cylindrical part and division by formation new poles. The protein complex responsible for septation during (the divisome) includes class A B penicillin-binding proteins (PBPs). In Escherichia coli, PBP3 is specific septal peptidoglycan synthesis. It requires putative lipid II flippase FtsW its localization at site necessary midcell PBP1B. this work we show direct interactions between...
In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking cell wall peptidoglycan during division. PBP3 mainly periplasmic, with 23 residues cytoplasmic tail and single transmembrane helix. We have solved crystal structure soluble form (PBP357–577) at 2.5 Å revealing two modules high molecular weight class B PBPs, carboxy terminal module exhibiting transpeptidase activity an amino unknown function. To gain...
Abstract The Arabidopsis splicing factor serine/arginine-rich 45 (SR45) contributes to several biological processes. sr45-1 loss-of-function mutant exhibits delayed root development, late flowering, unusual numbers of floral organs, shorter siliques with decreased seed sets, narrower leaves and petals, altered metal distribution. SR45 bears a unique RNA recognition motif (RRM) flanked by one (RS) domain on both sides. Here, we studied the function each domains examining their involvement in:...
Summary Zinc (Zn) excess negatively impacts primary root growth in Arabidopsis thaliana . Yet, the effects of Zn on specific processes tip (RT) remain largely unexplored. Transcriptomics, ionomics, and metabolomics were used to examine impact RT compared with remaining (RR). exposure resulted a shortened apical meristem elongation zone, differentiation initiating closer root. accumulated at lower concentration than RR. This pattern was associated expression homeostasis iron (Fe) deficiency...
Abstract Ser/Arg-rich (SR) proteins are essential nucleus-localized splicing factors. Our prior studies showed that Arabidopsis (Arabidopsis thaliana) RSZ22, a homolog of the human SRSF7 SR factor, exits nucleus through two pathways, either dependent or independent on XPO1 receptor. Here, we examined expression profiles and shuttling dynamics SRSF1 subfamily (SR30, SR34, SR34a, SR34b) under control their endogenous promoter in transient assay. Due to its rapid nucleocytoplasmic high level...
Zinc is an essential micronutrient for all living organisms. When challenged by zinc-limiting conditions, Arabidopsis thaliana plants use a strategy centered on two transcription factors, bZIP19 and bZIP23, to enhance the expression of several zinc transporters improve their uptake capacity. In cadmium hyperaccumulator plant halleri, highly efficient root-to-shoot translocation results in constitutive local deficiency roots high deficiency-responsive ZIP genes, supposedly boosting...
Although the involvement of Ser/Arg-rich (SR) proteins in RNA metabolism is well documented, their role vertebrate development remains elusive. We, therefore, elected to take advantage zebrafish model organism study SR genes' functions using splicing morpholino (sMO) microinjection and programmable site-specific nucleases. Consistent with previous research, we revealed discrepancies between mutant morphant phenotypes show that these inconsistencies may result from a large number unsuspected...
The plant serine/arginine-rich (SR) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation, and abscisic acid signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves petals, unusual numbers of floral organs. Here, we report that the presents an...
Natural variation among individuals and populations exists in all species, playing key roles response to environmental stress adaptation. Micro- macronutrients have a wide range of functions photosynthetic organisms, mineral nutrition thus plays sizable role biomass production. To maintain nutrient concentrations inside the cell within physiological limits prevent detrimental effects deficiency or excess, complex homeostatic networks evolved cells. The microalga Chlamydomonas reinhardtii...
Summary Zinc (Zn) excess negatively impacts primary root growth in Arabidopsis. Yet, the effects of Zn on specific processes tip remain largely unexplored. Transcriptomics, ionomics and metabolomics were used to examine impact (RT) compared remaining (RR). exposure resulted shortened apical meristem elongation zone, with differentiation initiating closer root. accumulated at a lower concentration RT than RR. This pattern was associated expression homeostasis Fe deficiency response genes. A...
Abstract The Arabidopsis splicing factors arginine/serine-rich zinc knuckle-containing proteins 32 and 33 (RS2Z32 RS2Z33) are plant-specific members of the SR family whose molecular functions received little attention. Here, we characterized both RS2Z32 RS2Z33 by examining their expression profile at different stages development spatial cellular distribution, as well contribution domains in establishment protein-protein interactions RNA binding specificity. We report that promoters...
Abstract The Arabidopsis splicing factor serine/arginine-rich 45 (SR45) contributes to several biological processes. sr45-1 loss-of-function mutant exhibits delayed root development, late flowering, unusual numbers of floral organs, shorter siliques with decreased seed sets, narrower leaves and petals, altered metal distribution. SR45 bears a unique RNA recognition motif (RRM) flanked by one (RS) domain on both sides. Here, we studied the function each domains examining their involvement in:...
Metallic micronutrients are essential throughout the plant life cycle. Maintaining metal homeostasis in tissues requires a highly complex and finely tuned network controlling uptake, transport, distribution storage. Zinc cadmium hyperaccumulation, such as observed model Arabidopsis halleri, represents an extreme evolution of this network. Here, non-ectopic overexpression A. halleri ZIP6 (AhZIP6) gene, encoding zinc influx transporter, thaliana enabled examining importance for flower...
Abstract The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves petals, unusual numbers of floral organs. Here, we report that the presents an...