A5091698550- Plant Molecular Biology Research
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Cellular transport and secretion
- Bacterial biofilms and quorum sensing
- Endoplasmic Reticulum Stress and Disease
- Plant Stress Responses and Tolerance
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Microbial Fuel Cells and Bioremediation
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Calcium signaling and nucleotide metabolism
- Genomics and Chromatin Dynamics
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- biodegradable polymer synthesis and properties
- Retinal Development and Disorders
- Chromosomal and Genetic Variations
- Nuclear Structure and Function
- Plant and soil sciences
- Plant Diversity and Evolution
- Educational methodologies and cognitive development
- Health and Lifestyle Studies
Fundación Ciencias Exactas y Naturales
2011-2024
University of Buenos Aires
2011-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
Ruhr University Bochum
2020-2023
Fundación Instituto Leloir
2015
Sequential protein posttranslational modifications facilitate cell wall self-assembly and root hair elongation in Arabidopsis .
Summary Root hairs (RH) are excellent model systems for studying cell size and polarity since they elongate several hundred‐fold their original size. Their tip growth is determined both by intrinsic environmental signals. Although nutrient availability temperature key factors a sustained plant growth, the molecular mechanisms underlying sensing downstream signaling pathways remain unclear. We use genetics to address roles of surface receptor kinase FERONIA (FER) TOR Complex 1 (TORC) in RH...
Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types organisms. Our interest was precisely to uncover spectrum loci regulated by a widespread plant factor involved physiological adaptation drought, type stress that plants have encountered since colonization land habitats 400 MYA. The regulator under study, named ASR1, exclusive kingdom (albeit absent Arabidopsis) and known alleviate caused restricted water availability. As its are...
Tolerance to water deficits was evolutionarily relevant the conquest of land by primitive plants. In this context, epigenetic events may have played important roles in establishment drought stress responses. We decided inspect marks plant organ that is crucial sensing stress: root. Using tomato as a crop model plant, we detected methylated epialleles Asr2, protein-coding gene widespread kingdom and thought alleviate restricted availability. found 3 contexts (CG, CNG, CNN) cytosines...
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, psychrotolerant psychrophilic microorganisms have developed different adaptation strategies to cope with stress derived from low temperatures. Pseudomonas extremaustralis an Antarctic bacterium able grow under temperatures produce high amounts polyhydroxyalkanoates (PHAs). In this work, we analyzed genome-wide transcriptome by RNA deep-sequencing...
Fasciclin-like arabinogalactan proteins (FLAs) are involved in numerous important functions plants but the relevance of their complex structure to physiological function and cellular fate is unresolved. Using a fully functional fluorescent version Arabidopsis thaliana FLA4 we show that this protein localized at plasma membrane as well endosomes soluble apoplast. likely be GPI-anchored, highly N-glycosylated carries two O-glycan epitopes previously associated with proteins. The activity was...
Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) cause a wide variety of bacterial infections coinfections, showing complex interaction that involves the production different metabolites metabolic changes. Temperature is key factor for survival virulence within host, bacteria could be exposed to an increment in temperature during fever development. We analyzed previously unexplored effect fever-like temperatures (39 °C) on S. USA300 P. PAO1 microaerobic mono- co-cultures compared...
Abstract Background Eukaryotic DNA methylation is one of the most studied epigenetic processes, as it results in a direct and heritable covalent modification triggered by external stimuli. In contrast to mammals, plant methylation, which stimulated cues exemplified various abiotic types stress, often found not only at CG sites but also CNG (N denoting A, C or T) CNN (asymmetric) sites. A genome-wide analysis Arabidopsis has shown that preferentially concentrated transposon genes non-coding...
Abstract Abortion of fertilized ovaries at the tip ear can generate significant yield losses in maize crops. To investigate mechanisms involved this process, 2 hybrids were grown field crops sowing densities and under 3 irrigation regimes (well‐watered control, drought before pollination, during pollination), all possible combinations. Samples tips taken 2–6 days after synchronous hand pollination used for analysis gene expression sugars. Glucose fructose levels increased kernels with high...
Mutants of the O-glycosylation pathway extensins as well molecular dynamics simulations uncover effects machinery on root hair tip growth.
Abstract Background Searching thoroughly for plant cis -elements corresponding to transcription factors is worthwhile reveal novel gene activation cascades. At the same time, a great deal of research currently focused on epigenetic events in plants. A widely used method serving both purposes chromatin immunoprecipitation, which was developed Arabidopsis and other plants but not yet operational tomato ( Solanum lycopersicum ), model species group economically important crops. Results We...
Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative envelopes, consisting several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance envelope characteristics adaptation in Antarctic bacterium Pseudomonas extremaustralis by analyzing mini Tn5 wapH mutant strain, encoding core LPS glycosyltransferase. Our results...
Root hairs (RHs) develop from specialized epidermal trichoblast cells, whereas cells that lack RHs are known as atrichoblasts. The mechanism controlling RH cell fate is only partially understood. regulated by a transcription factor complex promotes the expression of homeodomain protein GLABRA 2 (GL2), which blocks development inhibiting ROOT HAIR DEFECTIVE 6 (RHD6). Suppression GL2 activates RHD6, series downstream TFs including LIKE-4 (RSL4) and their target genes, causes to into RHs....
The ASR (for ABA/water stress/ripening) protein family, first described in tomato as nuclear and involved adaptation to dry climates, is widespread the plant kingdom, including crops of high agronomic relevance. We show both cytosolic localization for ASR1 (the most studied member family) histological samples by immunodetection, typically found small proteins readily diffusing through pores. Indeed, a was expected based on sorting prediction software, which also highlight monopartite signal...
Plants in arid zones are constantly exposed to drought stress. The ASR protein family (Abscisic, Stress, Ripening) -a subgroup of the late embryogenesis abundant superfamily- is involved water stress response and adaptation dry environments. Tomato ASR1, as well other members this family, an intrinsically disordered (IDP) that functions a transcription factor chaperone. Here we employed different biophysical techniques perform deep vitro characterization ASR1 IDP showed how both...
Significance The GET pathway is required for the insertion of tail-anchored (TA) membrane proteins in endoplasmic reticulum (ER) yeast and mammals. Some orthologous genes had also been identified higher plants with exception one two ER receptors insertion. Get2/CAML pathway’s cytosolic chaperone to dock release its TA protein cargo. Here we report identification elusive plant receptor through an interaction screen Arabidopsis . candidate allows detection further orthologs plants, revealing...
Abstract Major constituents of the plant cell walls are structural proteins that belong to hydroxyproline-rich glycoprotein (HRGP) family. Leucine-rich repeat extensin (LRX) contain a leucine-rich domain and C-terminal with repetitive Ser–Pro3–5 motifs potentially be O-glycosylated. It has been demonstrated pollen-specific LRX8–LRX11 from Arabidopsis thaliana necessary maintain integrity pollen tube wall during polarized growth. In HRGPs, including classical extensins (EXTs), probably in...
The final step in secretion is membrane fusion facilitated by SNARE proteins that reside opposite membranes. formation of a trans-SNARE complex between one R and three Q coiled-coiled domains drives the approach membranes providing mechanical energy for fusion. Biological control this mechanism exerted additional within some SNAREs. For example, N-terminal Longin domain (LD) R-SNAREs (also called Vesicle-associated proteins, VAMPs) can fold back onto blocking interaction with other cognate...
Halite (NaCl mineral) has exhibited the potential to preserve microorganisms for millions of years on Earth. This mineral was also identified Mars and in meteorites. In this study, we investigated halite crystals protect microbial life-forms surface an airless body (