A5072271579- Plant Molecular Biology Research
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Antibiotic Resistance in Bacteria
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Plant-Microbe Interactions and Immunity
- Peptidase Inhibition and Analysis
- Phytase and its Applications
- Inhalation and Respiratory Drug Delivery
- Enzyme Production and Characterization
- Antimicrobial Peptides and Activities
- Fungal Biology and Applications
Fundación Instituto Leloir
2015-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2023
Millennium Institute for Integrative Biology
2021
Fundación Ciencias Exactas y Naturales
2015-2017
University of Buenos Aires
2015-2017
Root hair polar growth is endogenously controlled by auxin and sustained oscillating levels of reactive oxygen species (ROS). These cells extend several hundred-fold their original size toward signals important for plant survival. Although final cell fundamental importance, the molecular mechanisms that control it remain largely unknown. Here we show ROS production transcription factor RSL4, which in turn transcriptionally regulated through response factors (ARFs). In this manner, controls...
Root Hairs (RHs) growth is influenced by endogenous and external environmental signals that coordinately regulate its final cell size. We have recently determined RH was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It proposed plasticity in response to temperature linked a reduced nutrient availability the media. Here, we explore molecular basis of this using Genome Wide Association Study (GWAS) approach natural accessions. identify poorly...
Root hair cells are important sensors of soil conditions. They grow towards and absorb water-soluble nutrients. This fast oscillatory growth is mediated by continuous remodeling the cell wall. walls contain polysaccharides hydroxyproline-rich glycoproteins, including extensins (EXTs). Class-III peroxidases (PRXs) secreted into apoplastic space thought to trigger either wall loosening or polymerization components, such as Tyr-mediated assembly EXT networks (EXT-PRXs). The precise role these...
ABSTRACT Root hair initiation is a highly regulated aspect of root development. The plant hormone ethylene and its precursor, 1-amino-cyclopropane-1-carboxylic acid, induce formation elongation hairs. Using confocal microscopy paired with redox biosensors dyes, we demonstrated that treatments elevate levels lead to increased hydrogen peroxide accumulation in cells prior formation. In the ethylene-insensitive receptor mutant, etr1-3, signaling double ein3eil1, increase number or reactive...
Mutants of the O-glycosylation pathway extensins as well molecular dynamics simulations uncover effects machinery on root hair tip growth.
Root hair size determines the surface area/volume ratio of whole roots exposed to nutrient and water pools, thereby likely impacting uptake rates. The speed at which they grow is determined both by cell-intrinsic factors like hormones (e.g., auxin) external environmental signals availability in soil phosphate). Overall root growth controlled transcription RSL4 RSL2. While high levels auxin promote growth, inorganic phosphate (Pi) media are able strongly repress RSL2 expression linked a...
Root hairs (RHs) develop from specialized epidermal trichoblast cells, whereas cells that lack RHs are known as atrichoblasts. The mechanism controlling RH cell fate is only partially understood. regulated by a transcription factor complex promotes the expression of homeodomain protein GLABRA 2 (GL2), which blocks development inhibiting ROOT HAIR DEFECTIVE 6 (RHD6). Suppression GL2 activates RHD6, series downstream TFs including LIKE-4 (RSL4) and their target genes, causes to into RHs....
Proline-rich extensin-like receptor kinases (PERKs) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily known be involved in many plant developmental processes. Here, we characterized two pollen-expressed PERKs from Arabidopsis thaliana, PERK5 and PERK12. Pollen tube growth was impaired single double perk5-1 perk12-1 loss of function mutants, with an impact on seed production. When segregation analysed, a male gametophytic defect found, indicating that mutants carry deficient...
Abstract Root hair cells are important sensors of soil conditions. Expanding several hundred times their original size, root hairs grow towards and absorb water-soluble nutrients. This rapid growth is oscillatory mediated by continuous remodelling the cell wall. walls contain polysaccharides hydroxyproline-rich glycoproteins including extensins (EXTs). Class-III peroxidases (PRXs) secreted into apoplastic space thought to trigger either wall loosening, oxygen radical species, or...
Abstract Plant genomes encode a unique group of papain-type Cysteine EndoPeptidases (CysEPs) containing KDEL endoplasmic reticulum (ER) retention signal (KDEL-CysEPs or CEPs). CEPs process the cell-wall scaffolding EXTENSIN proteins (EXTs), which regulate de novo cell wall formation and expansion. Since are able to cleave EXTs EXT-related proteins, acting as wall-weakening agents, they may play role in elongation. Arabidopsis thaliana genome encodes three (AtCPE1-AtCEP3). Here we report that...
Abstract Plant genomes encode a unique group of papain-type Cysteine EndoPeptidases (CysEPs) containing KDEL endoplasmic reticulum (ER) retention signal (KDEL-CysEPs or CEPs). CEPs process the cell-wall scaffolding EXTENSIN (EXT) proteins that regulate de novo formation and cell expansion. Since cleave EXTs EXT-related proteins, acting as cell-wall-weakening agents, they may play role in elongation. The Arabidopsis (Arabidopsis thaliana) genome encodes 3 (AtCPE1-AtCEP3). Here, we report...
Summary Root hairs (RHs) develop from specialized epidermal cells called trichoblasts, whereas that lack RHs are known as atrichoblasts. The mechanism controlling root cell fate is only partially understood. epidermis regulated by a transcription factor complex promotes the expression of homeodomain protein GLABRA 2 (GL2), which blocks RH development inhibiting ROOT HAIR DEFECTIVE 6 (RHD6). Suppression GL2 activates RHD6, series downstream TFs including LIKE-4 (RSL4 [Yi et al. 2010]) and...
Abstract Background Cell wall integrity plays an essential role during polarized cell growth typical of pollen tubes and root hairs. P roline-rich E xtensin-like R eceptor K inases (PERK) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily surface glycoproteins. Results Here, we identified two PERKs from Arabidopsis thaliana , PERK5 PERK12 highly expressed in mature pollen. Pollen tube was impaired single double perk5-1 perk12-1 loss function mutants, with a moderate impact on...
ABSTRACT Root hair initiation is a highly regulated aspect of root development. The plant hormone, ethylene, and its precursor, 1-amino-cyclopropane-1-carboxylic acid (ACC), induce formation elongation hairs. Using confocal microscopy paired with redox biosensors dyes, we demonstrated that treatments elevate ethylene levels led to increased hydrogen peroxide accumulation in cells prior formation. In two ethylene-insensitive mutants, etr1-3 ein3/eil1 , there was no increase number or ROS...
Abstract Root hair polar growth is endogenously controlled by auxin and sustained oscillating levels of reactive oxygen species (ROS). These cells extend several hundred-fold their original size toward signals important for plant survival. Although final cell fundamental importance, the molecular mechanisms that control it remain largely unknown. Here, we show ROS production transcription factors RSL4, which in turn transcriptionally regulated through Auxin Responsive Factors (ARFs). In this...
Here, we examined by which mechanism root hairs integrate conflicting growth-signals like the repressive high Pi-level clue and a concomitant auxin exposure that should promote growth questioned if these complex signals might activate known molecular players in polar growth.
ABSTRACT Root Hairs (RHs) growth is highly influenced by endogenous as well external environmental signals that coordinately regulate its final cell size. RHs actively expand the root surface responsible for nutrient uptake and water absorption. We have recently determined RH was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It proposed plasticity in response to temperature linked a reduced availability media. Here, we explored molecular basis...