A5035595645- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Food composition and properties
- Plant Reproductive Biology
- Genomics, phytochemicals, and oxidative stress
- Cassava research and cyanide
- Polysaccharides Composition and Applications
- Microbial Metabolites in Food Biotechnology
- Plant nutrient uptake and metabolism
- Enzyme Production and Characterization
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Biofuel production and bioconversion
- Transgenic Plants and Applications
- Moringa oleifera research and applications
- Plant Pathogens and Resistance
- Insect and Pesticide Research
- Porphyrin Metabolism and Disorders
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Potato Plant Research
University of Copenhagen
2015-2024
Planta
2015-2024
Aarhus University
2005-2012
Carlsberg Laboratory
1995-2012
Stanford University
2010
Carnegie Department of Plant Biology
2010
University of Michigan
2010
Max Planck Institute for Chemical Ecology
2006
Technical University of Munich
2006
Rothamsted Research
2003
Vascular plants appeared ~410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) lycophytes. We report here genome sequence lycophyte Selaginella moellendorffii (Selaginella), first nonseed vascular plant reported. By comparing gene content in evolutionarily diverse taxa, we found that transition from a gametophyte- to sporophyte-dominated life cycle required far fewer new genes than flowering plant, whereas secondary...
Sequential protein posttranslational modifications facilitate cell wall self-assembly and root hair elongation in Arabidopsis .
The use of pathogen-resistant cultivars is expected to increase yield and decrease fungicide in agriculture. However, potato breeding, increased resistance obtained via genes (R-genes) hampered because R-gene(s) are often specific for a pathogen race can be quickly overcome by the evolution pathogen. In parallel, susceptibility (S-genes) important pathogenesis, loss S-gene function confers several plants, such as rice, wheat, citrus tomatoes. this article, we present mutation screening seven...
Phenolic cross-links and inter-unit linkages result from the oxidative coupling of hydroxycinnamates leading to lignin assembly cross-linking with cell wall polysaccharides extensin proteins.
Abstract Glucosinolates are natural plant products that function in the defense toward herbivores and pathogens. Plant is regulated by multiple signal transduction pathways which salicylic acid (SA), jasmonic acid, ethylene as signaling molecules. Glucosinolate content was analyzed Arabidopsis wild-type plants response to single or combinatorial treatments with methyljasmonate (MeJA), 2,6-dichloro-isonicotinic ethylene, 2,4-dichloro-phenoxyacetic wounding. In addition, several mutants...
Abstract Camalexin represents the main phytoalexin in Arabidopsis (Arabidopsis thaliana). The camalexin-deficient deficient 3 (pad3) mutant has been widely used to assess biological role of camalexin, although exact substrate cytochrome P450 enzyme 71B15 encoded by PAD3 remained elusive. 2-(Indol-3-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid (dihydrocamalexic acid) was identified as likely intermediate camalexin biosynthesis downstream indole-3-acetaldoxime, it accumulated leaves silver...
In the glucosinolate pathway, postoxime enzymes have been proposed to low specificity for side chain and high functional group. Here, we provide biochemical evidence role of two cytochromes P450, CYP83A1 CYP83B1, from Arabidopsis in oxime metabolism biosynthesis glucosinolates. a detailed analysis substrate specificities recombinant heterologously expressed yeast (Saccharomyces cerevisiae), show that aliphatic oximes derived chain-elongated homologs methionine are efficiently metabolized by...
ObjectivesTo isolate a plant-derived compound with efflux inhibitory activity towards the NorA transporter of Staphylococcus aureus.
Abstract CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of endogenous U6 promoters. Highly increased efficiencies Granular Bound Starch Synthase gene at protoplast level obtained by replacement Arabidopsis promotor, driving expression CRISPR component, with promotors. This translated ex-plant into 35% full allelic editing. Indel Detection Amplicon Analysis was established as an efficient tool for fast assessment complex genomes, such potato....
Abstract Glucosinolates are a large group of plant secondary metabolites found mainly in the order Capparales, which includes number economically important Brassica crops and model Arabidopsis. In present study, several lines evidence provided for phloem transport glucosinolates When radiolabeled p-hydroxybenzylglucosinolate (p-OHBG) sucrose were co-applied to tip detached leaves, both tracers collected exudates at petioles. Long-distance [14C]p-OHBG was investigated wild-type transgenic...
Abstract Two homologous plant-specific Arabidopsis thaliana genes, RGXT1 and RGXT2, belong to a new family of glycosyltransferases (CAZy GT-family-77) encode cell wall (1,3)-α-d-xylosyltransferases. The deduced amino acid sequences contain single transmembrane domains near the N terminus, indicative type II membrane protein structure. Soluble secreted forms corresponding proteins expressed in insect cells showed xylosyltransferase activity, transferring d-xylose from UDP-α-d-xylose l-fucose....
We have characterized a β-glucuronosyltransferase (AtGlcAT14A) from Arabidopsis thaliana that is involved in the biosynthesis of type II arabinogalactan (AG). This enzyme belongs to Carbohydrate Active Enzyme database glycosyltransferase family 14 (GT14). The protein was localized Golgi apparatus when transiently expressed Nicotiana benthamiana. soluble catalytic domain Pichia pastoris transferred glucuronic acid (GlcA) β-1,6-galactooligosaccharides with degrees polymerization (DP) ranging...
Spike mosses are among the most basal vascular plants, and one species, Selaginella moellendorffii, was recently selected for full genome sequencing by Joint Genome Institute (JGI). Glycosyltransferases (GTs) involved in many aspects of a plant life, including cell wall biosynthesis, protein glycosylation, primary secondary metabolism. Here, we present comparative study S. moellendorffii across 92 GT families an additional family (DUF266) likely to include GTs. The encompasses moss...
Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses salinity, we developed and exploited real-time salt-induced tilting assay. This assay follows growth upon both gravitropic salt challenges, revealing that bending modulated by Na+ ions, but not osmotic stress. Next, measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions discovered genetic locus, encoding the cell wall-modifying enzyme EXTENSIN...
Summary Glucosinolates are natural products in cruciferous plants, including Arabidopsis thaliana . CYP79A1 is the cytochrome P450 catalysing conversion of tyrosine to p ‐hydroxyphenylacetaldoxime biosynthesis cyanogenic glucoside dhurrin sorghum. Both glucosinolates and glucosides have oximes as intermediates. Expression A. results production high levels tyrosine‐derived glucosinolate ‐hydroxybenzylglucosinolate, which not a constituent This provides further evidence that enzymes low...
Activated lymphocytes proliferate, secrete cytokines, and can make antibodies. Normally activated B T cells meet the bioenergetic demand for these processes by up-regulating aerobic glycolysis. In contrast, several lines of evidence suggest that pathogenic in autoimmune diseases like lupus ATP demands through oxidative phosphorylation. Using 13 C-glucose as a stable tracer, we found splenocytes from mice with derive same fraction lactate glucose control animals, suggesting comparable levels...