A5084871360- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Machine Learning in Bioinformatics
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Polysaccharides and Plant Cell Walls
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- Ion Channels and Receptors
- Plant-Microbe Interactions and Immunity
- Seed Germination and Physiology
- Hearing, Cochlea, Tinnitus, Genetics
- Plant tissue culture and regeneration
- Bioinformatics and Genomic Networks
- Light effects on plants
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Biochemical Analysis and Sensing Techniques
- Plant and animal studies
- Antioxidant Activity and Oxidative Stress
- Biochemical and biochemical processes
- Cancer Research and Treatments
- Connexins and lens biology
Zero to Three
2024
Experimental Medicine and Biology Institute
2003-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2003-2024
Stanford University
2014-2024
Howard Hughes Medical Institute
2012-2023
Shanghai Institutes for Biological Sciences
2008
Center for Excellence in Molecular Plant Sciences
2008
Plant Gene Expression Center
2005-2008
United States Department of Agriculture
2005-2008
Agricultural Research Service
2005
The pollen-specific receptor kinases LePRK1 and LePRK2 have localization expression profiles that strongly suggest they play roles in pollen germination tube growth. To identify downstream components of LePRK signaling, we used their cytoplasmic domains (CDs) as baits yeast two-hybrid screens a tomato cDNA library. A protein named kinase partner (KPP) interacted with the CDs both an vitro pull-down assay, co-immunoprecipitation assay. KPP is peripheral membrane phosphorylated pollen. Pollen...
Plant cells maintain remarkable developmental plasticity, allowing them to clonally reproduce and repair tissues following wounding; yet plant normally stably consistent identities. Although this capacity was recognized long ago, our mechanistic understanding of the establishment, maintenance, erasure cellular identities in plants remains limited. Here, we develop a cell-type–specific reprogramming system that can be probed at genome-wide scale for alterations gene expression histone...
Abstract In flowering plants, the process of pollen germination and tube growth is required for successful fertilization. A receptor kinase from tomato (Solanum lycopersicum), LePRK2, has been implicated in signaling during as well mediating (tube)-pistil communication. Here we show that reduced expression LePRK2 affects four aspects growth. First, percentage germinates reduced, time window competence to germinate also shorter. Second, rate both vitro pistil. Third, tip-localized superoxide...
After pollen grains germinate on the stigma, tubes traverse extracellular matrix of style their way to ovules. We previously characterized two pollen-specific, receptor-like kinases, LePRK1 and LePRK2, from tomato ( Lycopersicon esculentum ). Their structure immunolocalization pattern specific dephosphorylation LePRK2 suggested that these kinases might interact with signaling molecules in matrix. Here, we show can be coimmunoprecipitated or when expressed together yeast. In yeast,...
Abstract When multiple mitogen-activated protein kinase (MAPK) components are recruited recurrently to transduce signals of different origins, and often opposing outcomes, mechanisms enforce signaling specificity utmost importance. These largely uncharacterized in plant MAPK networks. The Arabidopsis thaliana stomatal lineage was previously used show that when rendered constitutively active, four kinases (MKKs), MKK4/5/7/9, capable perturbing development these comprise two pairs, MKK4/5...
Abstract Background LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they present in a high molecular weight complex (LePRK complex). is phosphorylated mature germinated pollen, but dephosphorylated when membranes incubated with tomato or tobacco style extracts. Results Here we show that dephosphorylation mediated by heat-, acid-, base-, DTT- protease-resistant component from styles. Using phosphorylation as tracking assay for purification, exudates...
Nicotiana benthamiana is a valuable plant chassis for heterologous production of medicinal natural products. This host well suited the processing organelle-localized enzymes, and conservation primary metabolism across kingdom often provides required plant-specific precursor molecules that feed given pathway. Despite this commonality in metabolism, limited supply and/or competing pathways can interfere with yields Here, we use transient transcriptional reprogramming endogenous N. to...
Abstract Zinc (Zn) deficiency is recognized as a global crisis it observed in half of all agricultural soils. However, the molecular mechanisms that drive plant physiological responses to soil Zn are not well understood. We used an untargeted metabolomics approach search for metabolites exuded from roots during stress, which led discovery collection secreted small defensin-like peptides Arabidopsis thaliana (named Zinc-Deficiency Responsive Peptides (ZDRPs)). Phylogenetic analysis and...
The tip-growing pollen tube is a useful model for studying polarized cell growth in plants. We previously characterized LePRK2, pollen-specific receptor-like kinase from tomato (1). Here, we showed that LePRK2 present as multiple phosphorylated isoforms mature membranes. Using comparative sequence analysis and phosphorylation site prediction programs, identified two putative motifs the cytoplasmic juxtamembrane (JM) domain. Site-directed mutagenesis these motifs, followed by transient...
For normal cochlear function, outer hair cells (OHCs) require a precise control of intracellular Ca<sup>2+</sup> levels. In the absence regulatory elements such as proteinaceous buffers or extrusion pumps, OHCs degenerate, leading to profound hearing impairment. Influx occurs both at stereocilia tips and basolateral membrane. this latter compartment, two different origins for influx have been poorly explored: voltage-gated L-type channels (VGCCs) synapses with Type II afferent neurons, α9α10...
Abstract Major constituents of the plant cell walls are structural proteins that belong to hydroxyproline-rich glycoprotein (HRGP) family. Leucine-rich repeat extensin (LRX) contain a leucine-rich domain and C-terminal with repetitive Ser–Pro3–5 motifs potentially be O-glycosylated. It has been demonstrated pollen-specific LRX8–LRX11 from Arabidopsis thaliana necessary maintain integrity pollen tube wall during polarized growth. In HRGPs, including classical extensins (EXTs), probably in...
Mitogen-activated protein kinases (MAPK) signaling affects many processes, some of which have different outcomes in the same cell. In Arabidopsis, activation a MAPK cascade consisting YODA, MKK4/5 and MPK3/6 inhibits early stages stomatal developmental, but ability to halt progression is lost at later stage when guard mother cells (GMCs) transition (GCs). Rather than downregulating components, precursors must mechanism prevent late inhibition because MKKs MPKs mediate other physiological...
SUMMARY Rapid hypocotyl elongation allows buried seedlings to emerge, where light triggers de‐etiolation and inhibits growth mainly by photoreceptors. Phosphorylation/dephosphorylation events regulate many aspects of plant development. Only recently we have begun uncover the earliest phospho‐signaling responders light. Here, reported a large‐scale phosphoproteomic analysis identified 20 proteins that changed their phosphorylation pattern following min pulse compared darkness....
Abstract With the increasing availability of single-cell transcriptomes, RNA signatures offer a promising basis for targeting living cells. Molecular sensors would enable study and therapeutic interventions specific cell types/stats in diverse contexts, particularly human patients non-model organisms. Here we describe modular programmable design live R NA sensing using denosine d eaminases cting on (RADAR). We validated then expanded our basic design, characterized its performance,...
In recent years, the rapid alkalinization factor (RALF) family of cysteine-rich peptides has been reported to be crucial for several plant signaling mechanisms, including cell growth, immunity and fertilization. RALF4 RALF19 (RALF4/19) pollen redundantly regulate tube integrity growth through binding their receptors ANXUR1/2 (ANX1/2) Buddha's Paper Seal 1 2 (BUPS1/2), members
Abstract Major constituents of the plant cell walls are structural proteins that belong to Hydroxyproline-rich glycoprotein family. Leucine-rich repeat extensis contain a leucine-rich domain and C-terminal with repetitive Ser-Pro(3-5) motifs plausible be glycosylated. We have demonstrated pollen-specific LRX8-11 from Arabidopsis thaliana necessary maintain integrity pollen tube wall during polarized growth. In classical EXTs likely in LRXs, proline residues converted hydroxyproline by...