A5018684468- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- Cancer-related gene regulation
- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Neurogenetic and Muscular Disorders Research
- Genetic Syndromes and Imprinting
- Cellular Mechanics and Interactions
- Genetic and Kidney Cyst Diseases
- RNA regulation and disease
- Estrogen and related hormone effects
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Trypanosoma species research and implications
- Chemical Synthesis and Analysis
Fundación Ciencias Exactas y Naturales
2014-2024
University of Buenos Aires
2014-2024
Institute of Astronomy and Space Physics
2017-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2021
Federation of American Societies for Experimental Biology
2020
Instituto de Neurología de Buenos Aires
2019-2020
National Institutes of Health
2020
Yale University
2020
Harvard University
2020
Rockefeller University
2020
Light is a source of energy and also regulator plant physiological adaptations. We show here that light/dark conditions affect alternative splicing subset Arabidopsis genes preferentially encoding proteins involved in RNA processing. The effect requires functional chloroplasts observed roots when the communication with photosynthetic tissues not interrupted, suggesting signaling molecule travels through plant. Using electron transfer inhibitors different mechanisms action, we deduce reduced...
It has been assumed that constitutive and regulated splicing of RNA polymerase II transcripts depends exclusively on signals present in the molecule. Here we show changes promoter structure strongly affect splice site selection. We investigated ED I exon, which encodes a facultative type III repeat fibronectin, whose inclusion is during development proliferative processes. used an alternative assay combined with swapping to demonstrate extent dependent from transcript originated this...
A Lavigueur, H La Branche, R Kornblihtt, and B Chabot Département de Microbiologie, Faculté Médecine, Université Sherbrooke, Québec, Canada.
In search for physiological pathways affecting alternative splicing through its kinetic coupling with transcription, we found that membrane depolarization of neuronal cells triggers the skipping exon 18 from neural cell adhesion molecule (NCAM) mRNA, independently calcium/calmodulin protein kinase IV pathway. We show this responds to RNA polymerase II elongation, because inclusion is increased by a slow mutant. Depolarization affects chromatin template in specific way, causing H3K9...
Journal Article Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains dfffering in the number of internal repeats Get access Alberto R. Kornblihtt, Kornblihtt * Sir William Dunn School Pathology, University OxfordSouth Parks Road, Oxford OX1 3RE, UK *To whom reprint requests should be sent Search for other works by this author on: Academic PubMed Google Scholar Karen Vibe-Pedersen, Vibe-Pedersen Fransciso E. Baralle Nucleic Acids Research, Volume 12, Issue...
Promoter and enhancer elements can influence alternative splicing, but the basis for this phenomenon is not well understood. Here we investigated how different transcriptional activators affect decision between inclusion exclusion (skipping) of fibronectin EDI exon. A mutant acidic VP16 activation domain called SW6 that preferentially inhibits polymerase II (pol II) elongation caused a reduction in exon skipping. Exon skipping was fully restored presence by either SV40 cis or human...