A5082875717- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Neurogenetic and Muscular Disorders Research
- Reproductive Biology and Fertility
- Amyotrophic Lateral Sclerosis Research
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sarcoma Diagnosis and Treatment
- Renal and related cancers
- Cancer-related gene regulation
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- DNA Repair Mechanisms
- Exercise and Physiological Responses
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Prostate Cancer Treatment and Research
- Mast cells and histamine
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
Istituti di Ricovero e Cura a Carattere Scientifico
2005-2025
Foro Italico University of Rome
2016-2025
Fondazione Santa Lucia
2016-2025
Bambino Gesù Children's Hospital
2021
Sapienza University of Rome
2001-2015
Centre for Genomic Regulation
2009-2014
University of Rome Tor Vergata
2002-2013
Durham University
2013
Newcastle University
2013
University of Leicester
2013
The RNA-binding protein Sam68 is involved in apoptosis, but its cellular mRNA targets and mechanism of action remain unknown. We demonstrate that binds the for Bcl-x affects alternative splicing. Depletion by RNA interference caused accumulation antiapoptotic Bcl-x(L), whereas up-regulation increased levels proapoptotic Bcl-x(s). Tyrosine phosphorylation Fyn inverted this effect favored Bcl-x(L) splice site selection. A point mutation domain influenced splicing activity subnuclear...
Human cyclin D1 is expressed as two isoforms derived by alternate RNA splicing, termed D1a and D1b, which differ for the inclusion of intron 4 in D1b mRNA. Both are frequently upregulated human cancers, but displays relatively higher oncogenic potential. The splicing factors that regulate alternative remain unknown despite likelihood they contribute to oncogenicity. In this study, we report Sam68, an RNA-binding protein overexpressed prostate cancer cells, enhances supports its expression...
In the mouse, three genes that are homologous to Drosophila Nanos (Nos) gene have been identified. Deletion of one these genes, Nanos2, results in male sterility, owing loss germ cells during fetal life. Before apoptosis, Nanos2-null gonocytes enter meiosis, suggesting Nanos2 functions as a meiotic repressor. Here, we show is continuously expressed from postnatal spermatogonial stem cells. We observed promeiotic factor AtRA, an analog retinoic acid (RA), downregulates NANOS2 levels, both and...
Sam68 is a KH-type RNA-binding protein involved in several steps of RNA metabolism with potential implications cell differentiation and cancer. However, its physiological roles are still poorly understood. Herein, we show that Sam68−/− male mice infertile display defects spermatogenesis, demonstrating an essential role for fertility. produce few spermatozoa, which dramatic motility unable to fertilize eggs. Expression subset messenger mRNAs (mRNAs) affected the testis knockout mice....
Deregulation of the phosphatidyl inositol trisphosphate kinase/AKT/mammalian target rapamycin (mTOR) and RAS/mitogen-activated protein kinase (MAPK)/MNK pathways frequently occurs in human prostate carcinomas (PCas) leads to aberrant modulation messenger RNA (mRNA) translation. We have investigated relative contribution these translational regulation proliferation PCa cells. MNK-dependent phosphorylation eIF4E is elevated DU145 cells, which low basal levels AKT/mTOR activity due expression...
Abstract NEK2 is a serine/threonine kinase that promotes centrosome splitting and ensures correct chromosome segregation during the G2/M phase of cell cycle, through phosphorylation specific substrates. Aberrant expression activity in cancer cells lead to dysregulation cycle aneuploidy. Thus, tight regulation function needed progression. In this study, we found localizes nucleus derived from several tissues. particular, co-localizes splicing speckles with SRSF1 SRSF2. Moreover, interacts...
Abstract Meiosis requires conserved transcriptional changes, but it is not known whether there a corresponding set of RNA splicing switches. Here, we used RNAseq mouse testis to identify changes associated with the progression from mitotic spermatogonia meiotic spermatocytes. We identified ∼150 switches, most which affect protein-coding exons. The expression many key regulators changed in course meiosis, including downregulation polypyrimidine tract binding protein (PTBP1) and heterogeneous...
Ewing sarcomas (ES) are biologically aggressive tumors of bone and soft tissues for which no cure is currently available. Most ES patients do not respond to chemotherapeutic treatments or acquire resistance. Since the PI3K/AKT/mTOR axis often deregulated in ES, its inhibition offers therapeutic perspective these tumors. Herein, by using splicing sensitive arrays, we have uncovered an extensive program activated upon signaling pathway BEZ235. Bioinformatics analyses identified hnRNPM as a key...
Abstract Background Advanced prostate cancer (PC) is characterized by insensitivity to androgen deprivation therapy and chemotherapy, resulting in poor outcome for most patients. Thus, advanced PC urgently needs novel therapeutic strategies. Mounting evidence points splicing dysregulation as a hallmark of PC. Moreover, pharmacologic inhibition the process emerging promising option this disease. Method By using representative androgen-insensitive cell line (22Rv1), we have investigated...