A5059274674- Cancer Mechanisms and Therapy
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- interferon and immune responses
- MXene and MAX Phase Materials
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Cardiomyopathy and Myosin Studies
- Cytokine Signaling Pathways and Interactions
- Mechanisms of cancer metastasis
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Pancreatitis Pathology and Treatment
- Connective tissue disorders research
- Cell death mechanisms and regulation
- Toxin Mechanisms and Immunotoxins
- Proteoglycans and glycosaminoglycans research
- Nanoparticles: synthesis and applications
- T-cell and Retrovirus Studies
- Trace Elements in Health
- Microbial Inactivation Methods
- Galectins and Cancer Biology
University of Helsinki
2000-2023
Tampere University
2007-2017
Tampere University Hospital
2007-2017
Tampere University of Applied Sciences
2009-2010
Umeå University
1998-2009
Activating germline mutations in STAT3 were recently identified as a cause of neonatal diabetes mellitus associated with beta-cell autoimmunity. We have investigated the effect an activating mutation, STAT3K392R, on pancreatic development using induced pluripotent stem cells (iPSCs) derived from patient and hypoplasia. Early endoderm differentiated similarly STAT3K392R healthy-control cells, but later stages, NEUROG3 expression was upregulated prematurely together insulin (INS) glucagon...
E2A, HEB and E2–2 genes encode a group of basic helix-loop-helix (bHLH) transcription factors that are structurally functionally similar. Deletion the encoding either these proteins leads to early lethality block in B lymphocyte development. Evidence for function T development has, however, only been reported E2A HEB. To further elucidate role at developmental stages have proven difficult study due phenotype mice defective E2–2, we generated analyzed conditionally mutated gene. These mosaic...
MINT‐7968768, MINT‐7968779: Tudor‐SN (uniprotkb:Q7KZF4) physically interacts (MI:0915) with G3BP (uniprotkb:Q13283) by anti bait coimmunoprecipitation (MI:0006) MINT‐7968800: and TIA‐1 (uniprotkb:P31483) colocalize (MI:0403) fluorescence microscopy (MI:0416) MINT‐7968789:
Stress granules (SGs) and processing bodies (PBs) comprise the main types of cytoplasmic RNA foci during stress. Our previous data indicate that knockdown human Tudor staphylococcal nuclease (Tudor-SN) affects aggregation SGs. However, precise molecular mechanism has not been determined fully. In present study, we demonstrate Tudor-SN binds colocalizes with many core components SGs, such as poly(A)(+) mRNA binding protein 1, T-cell internal antigen-1-related mRNA, SG/PB sharing proteins...
Upon encountering antigens, B-lymphocytes can adapt to produce a highly specific and potent antibody response. Somatic hypermutation, which introduces point mutations in the variable regions of genes, increase affinity for antigen, effector functions be altered by class switch recombination (CSR), changes expressed constant region exons. Activation-induced cytidine deaminase (AID) is mutagenic diversification enzyme that essential both somatic hypermutation CSR. The AID has tightly...
Snd1 is an evolutionarily conserved RNA-binding protein implicated in several regulatory processes gene expression including activation of transcription, mRNA splicing, and microRNA decay. Here, we have investigated the outcome
The Ca2+ sensor protein calmodulin can interact with the DNA binding basic helix-loop-helix (bHLH) domain of E12, E47, and SEF2-1 (E2-2), which belong to E-protein subclass bHLH transcription factors. This interaction inhibits these proteins in vitro, an ionophore that increases intracellular inhibit transcriptional activation by E-proteins. Here we have attempted determine if phenomena reflect a direct calmodulin-dependent inhibition E-proteins vivo. We show overexpression activity SEF2-1....
The JAK/STAT pathway is essential for organogenesis, innate immunity, and stress responses in Drosophila melanogaster. its associated regulators have been highly conserved evolution from flies to humans. We used a genome-wide RNAi screen S2 cells identify of the pathway, here we report characterization Not4 as positive regulator pathway. Overexpression enhanced Stat92E-mediated gene vitro vivo Drosophila. Specifically, increased reporter activation cells; flies, overexpression resulted an...
The members of the MyoD family basic helix-loop-helix (bHLH) transcription factors are critical regulators skeletal muscle differentiation that function as heterodimers with ubiquitously expressed E-protein bHLH factors. These must compete successfully homodimers E12 and other E-proteins to enable myogenesis. Here, we show mutants resistant Ca(2+)-loaded calmodulin (CaM) inhibit MyoD-initiated myogenic conversion transfected fibroblasts. Ca(2+) channel blockers reduce, stimulation increases,...
Regulation of transcription requires cooperation between sequence-specific factors and numerous coregulatory proteins. In IL-4/IL-13 signaling several coactivators for STAT6 have been identified, but the molecular mechanisms STAT6-mediated gene are still not fully understood. Here we identified by proteomic approach that PTB-associated splicing factor (PSF) interacts with STAT6. intact cells interaction was observed only after IL-4 stimulation. The IL-4-induced tyrosine phosphorylation both...
Tudor-SN is a multifunctional regulator of gene expression that has been shown to function as transcriptional co-activator, miRNA processing, mRNA splicing, and stability. also identified component in RNA-induced silencing complex. Here we have produced characterized seven monoclonal antibody (MAb) clones against human Tudor-SN. Antibodies were generated the fourth staphylococcal nuclease-like domain (SN4) Tudor The MAbs recognize protein Western blot analysis immunoprecipitation, detect...
SND1 is an RNA-binding protein overexpressed in large variety of cancers. has been proposed to enhance stress tolerance cancer cells, but the molecular mechanisms are still poorly understood. We analyzed expression 372 miRNAs colon carcinoma cell line and show that silencing increases levels several tumor suppressor miRNAs. Furthermore, knockdown showed synergetic effects with drugs through MEK-ERK Bcl-2 family-related apoptotic pathways. To explore whether SND1-mediated RNA...
AbstractA general approach to address the biological function of a calcium-binding protein, or another in living cells is increase decrease activity protein cell and analyze effects on functions. In many cases, it desirable determine overexpressing constitutively active dominantly negative derivative, express that normally lacks it. This achieved by introducing its gene exogenously. The cDNA for cloned downstream an promoter plasmid designed expression mammalian cells. then transfected into...