A5009577914- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Taxonomy and Phylogenetics
- Genetically Modified Organisms Research
- Plant Pathogens and Resistance
- Potato Plant Research
- Plant Disease Resistance and Genetics
- Environmental Toxicology and Ecotoxicology
- Forest ecology and management
- Transgenic Plants and Applications
- Animal Genetics and Reproduction
- Cell death mechanisms and regulation
- Agricultural safety and regulations
- Vanadium and Halogenation Chemistry
- Genomics and Phylogenetic Studies
- International Maritime Law Issues
- Mechanisms of cancer metastasis
- Bioenergy crop production and management
- Nanoparticles: synthesis and applications
- Genomics and Chromatin Dynamics
Umeå Plant Science Centre
2025
Swedish University of Agricultural Sciences
2012-2024
Uppsala University
1996-2011
Evolutionary Genomics (United States)
2011
Yale University
2002-2006
The phosphatidyl ethanolamine-binding protein (PEBP) gene family is present in all eukaryote kingdoms, with three subfamilies identified angiosperms (FLOWERING LOCUS T [FT], MOTHER OF FT AND TFL1 [MFT], and TERMINAL FLOWER1 [TFL1] like). In angiosperms, PEBP genes have been shown to function both as promoters suppressors of flowering control plant architecture. this study, we focus on previously uncharacterized from gymnosperms. Extensive database searches suggest that gymnosperms possess...
Abstract The establishment and maintenance of auxin maxima in vascular plants is regulated by biosynthesis polar intercellular flow. disruption normal mouse-ear cress (Arabidopsis thaliana) leads to severe abnormalities, suggesting that spatiotemporal regulation fundamental for growth development. We have shown previously the induction SHORT-INTERNODES/STYLISH (SHI/STY) family member STY1 results increased transcript levels YUCCA (YUC) YUC4 also higher rates Arabidopsis seedlings. SHI/STY...
MIKCc-type MADS-box genes encode key transcriptional regulators of a variety developmental processes in Arabidopsis thaliana. However, there has been relatively little effort to systematically carry out comparative genomic or functional analyses these across flowering plants. Here we describe strategy identify members the gene family from any angiosperm species interest. Using this approach, have identified 24 tomato, including 17 that not previously characterized. sequences, performed...
Global food security is one of the most pressing issues for humanity, and agricultural production critical achieving this. The existing analyses specific threats to seldom bring out contrasts associated with different levels economic development climatic zones. We therefore investigated same biophysical in three modelled types countries conditions. analysed were environmental degradation, climate change diseases pests animals plants. These a methodology enabling risks be compared. timeframe...
Summary The Norway spruce MADS‐box genes DAL11 , DAL12 and DAL13 are phylogenetically related to the angiosperm B‐function genes: that act together with A‐function in specifying petal identity C‐function stamen floral organs. In this report we present evidence suggest B‐gene function specification of pollen‐bearing organs has been conserved between conifers angiosperms. Expression or transgenic Arabidopsis causes phenotypic changes which partly resemble those caused by ectopic expression...
Summary The floral homeotic gene APETALA1 ( AP1 ) specifies meristem identity and sepal petal in Arabidopsis. Consistent with its multiple roles during development, is initially expressed throughout the meristem, later expression becomes restricted to primordia. Using chromatin immunoprecipitation, we show that PISTILLATA (PI) protein, required for stamen has ability bind directly promoter region of . In support hypothesis PI, interacting partner APETALA3 (AP3), regulates transcription ,...
Somatic embryogenesis is used for vegetative propagation of conifers. Embryogenic cultures can be established from zygotic embryos; however, the embryogenic potential decreases during germination. In Arabidopsis, LEAFY COTYLEDON (LEC) genes are expressed embryonic stage, and must repressed to allow Treatment with histone deacetylase inhibitor trichostatin A (TSA) causes de-repression LEC genes. ABSCISIC ACID3 (ABI3) its Zea mays ortholog VIVIPAROUS1 (VP1) act together promote embryo...
Summary Reproductive organs in seed plants are morphologically divergent and their evolutionary history is often unclear. The mechanisms controlling development have been extensively studied angiosperms but poorly understood conifers other gymnosperms. Here, we address the molecular control of cone N orway spruce, P icea abies . We present expression analyses five novel MADS ‐box genes comparison with previously identified LEAFY at distinct developmental stages. In addition, characterized...
As multiple UN fora develop parallel rules for sharing benefits from digital sequence information, we urge better coordination. International policymakers should focus on harmonizing new benefit-sharing to ensure open access data, database interoperability, and benefit outcomes.
Summary To gain new insights into the mechanism underlying cytoplasmic male sterility (CMS), we compared nuclear gene expression profiles of flowers a Brassica napus CMS line with that fertile B. maintainer using Arabidopsis thaliana flower‐specific cDNA microarrays. The used has genome, but rearranged mitochondrial (mt) genome consisting both and A. DNA. Gene profiling revealed large number genes differed in between two lines. For example, coding for proteins are involved protein import...
During embryo development in most gymnosperms, the establishment of shoot apical meristem (SAM) occurs concomitantly with formation a crown cotyledons surrounding SAM. It has previously been shown that differentiation somatic embryos Picea abies is dependent on polar auxin transport (PAT). In angiosperm model plant, Arabidopsis thaliana, cotyledonary boundaries and embryonal SAM PAT expression CUP-SHAPED COTYLEDON (CUC) genes, which belong to large NAC gene family. The aim this study was...
Abstract Rice cultivation on paddy soil is commonly associated with emissions of methane, a greenhouse gas, but rice varieties may differ in their actual level emissions. This study analysed methane 22 distinct genotypes, using gas chromatography, and identified the cultivar Heijing 5 from northern China as potential low-methane variety. To confirm this to examine whether can perform similarly at higher latitudes, was cultivated field trials (lat. 32° N) Sweden 59° where (i) were measured,...
Global rice cultivation significantly contributes to anthropogenic methane emissions. The emissions are caused by methane-producing microorganisms (methanogenic archaea) that favoured the anoxic conditions of paddy soils and small carbon molecules released from roots. However, different cultivars associated with differences in emission rates suggesting there is a considerable natural variation this trait. Starting hypothesis sugar allocation within plant an important factor influencing both...
• In orthodox seeds, the transcriptional activator ABI3 regulates two major stages in embryo maturation: a mid-maturation (MAT) stage leading to accumulation of storage compounds, and late maturation (LEA) quiescence desiccation tolerance. Our aim was elucidate mechanisms for shutdown MAT genes during maturation, better understand phase transition between LEA stages. Using transgenic transient approaches Nicotiana, we examined activities ABI3-dependent reporter driven by multimeric RY...
Abstract Conifers normally go through a long juvenile period, for Norway spruce (Picea abies) around 20 to 25 years, before developing male and female cones. We have grown plants from inbred crosses of naturally occurring mutant (acrocona). One-fourth the segregating acrocona initiate cones already in their second growth cycle, suggesting control by single locus. The early cone-setting properties were utilized identify candidate genes involved vegetative-to-reproductive phase change spruce....
Co-expression of physically linked genes occurs surprisingly frequently in eukaryotes. Such chromosomal clustering may confer a selective advantage as it enables coordinated gene regulation at the chromatin level. We studied organization involved male reproductive development Arabidopsis thaliana. developed an in-silico tool to identify physical clusters co-regulated from expression data. identified 17 (96 genes) stamen and acting downstream transcriptional activator MS1 (MALE STERILITY 1),...
Societal Impact Statement Can modern breeding technologies, such as genome editing, contribute to reduced pesticide usage? This question has been accentuated by a recent legal proposal exempt genome‐edited plants from the strict regulations applied classical genetically modified (GM) crops within European Union (EU). Using official statistics on crop cultivation and usage for two example commonly grown in Sweden, we calculate that cereal farmers collectively could save up 70 million € wheat...
The European Commission proposal for New Genomic Techniques (NGTs) of July 2023 specifies that NGT1 plants, which are considered equivalent to conventional may differ from the recipient or parental plant by no more than 20 insertions, cannot be longer bp; deletions can any size and number. Here, we examine proposed 20/20 limit against background theoretical considerations older data used frame it in light recent highly contiguous long-read assemblies reference genomes pangenomes. We find...
<title>Abstract</title> Norway spruce (Picea abies L. Karst) and Scots pine (Pinus sylvestris L.) are the dominant tree species in northern Eurasia with global importance for carbon sequestration that increasingly at risk from climate change. Here, we present chromosome-scale reference genomes both an unprecedented conifer resequencing effort comprising over 1000 trees. We show that, despite diverging ~130 million years ago, contrast to angiosperms, retain remarkable macro-synteny no signs...
The high-throughput expression analysis technologies available today give scientists an overflow of profiles but their resolution in terms tissue specific is limited because problems dissecting individual tissues. Expression data needs to be confirmed and complemented with patterns using e.g. situ hybridization, a technique used localize cell mRNA expression. hybridization method laborious, time-consuming often requires extensive optimization depending on species tissue. In experiments are...
Antisense oligodeoxynucleotide (asODN) inhibition was developed in the 1970s, and since then has been widely used animal research. However, plant biology, method had limited application because cell walls significantly block efficient uptake of asODN to cells. Recently, we have found that is enhanced a sugar solution. The promise for many applications, such as rapid alternative time-consuming transgenic studies, high potential studying gene functionality intact plants multiple species, with...