A5089421438- biodegradable polymer synthesis and properties
- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Microplastics and Plastic Pollution
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Peroxisome Proliferator-Activated Receptors
- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Plant Virus Research Studies
- Composting and Vermicomposting Techniques
- Microbial Metabolites in Food Biotechnology
- Advanced oxidation water treatment
- Toxic Organic Pollutants Impact
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Biofuel production and bioconversion
- Electrospun Nanofibers in Biomedical Applications
- Poxvirus research and outbreaks
- Porphyrin Metabolism and Disorders
- Pharmacological Effects of Natural Compounds
- Advanced Polymer Synthesis and Characterization
- Enzyme Production and Characterization
University of Buenos Aires
2012-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2001-2024
Fundación Ciencias Exactas y Naturales
2012-2024
Instituto de Química y Fisicoquímica Biológicas
2014-2020
Temperature is one of the most important factors for bacterial growth and development. Cold environments are widely distributed on earth, psychrotolerant psychrophilic microorganisms have developed different adaptation strategies to cope with stress derived from low temperatures. Pseudomonas extremaustralis an Antarctic bacterium able grow under temperatures produce high amounts polyhydroxyalkanoates (PHAs). In this work, we analyzed genome-wide transcriptome by RNA deep-sequencing...
ABSTRACT Pseudomonas extremaustralis 14-3b presents genes involved in the synthesis of different polyhydroxyalkanoates, tolerance and degradation pollutants, microaerobic metabolism. Several genomic islands were detected. Genetic machinery could contribute to adaptability stressful conditions. This is first genome sequence reported from a isolated cold environments.
To determine whether the stationary sigma factor, σS, influences polyhydroxyalkanoate metabolism in Pseudomonas putida KT2440, an rpoS-negative mutant was constructed to evaluate accumulation and expression of a translational fusion promoter region genes that code for synthase 1 (phaC1) depolymerase (phaZ). By comparison with wild-type, rpoS showed higher degradation rate increased during growth phase. These results suggest σS might control involved metabolism, possibly indirect manner. In...
Adipogenesis is stimulated in 3T3-L1 fibroblasts by a combination of insulin, dexamethasone and isobutylmethylxanthine, IBMX, (I+D+M). Two transcription factors are important for the acquisition adipocyte phenotype, C/EBP beta (CCAT enhancer-binding protein beta) PPAR gamma (peroxisome proliferator-activated receptor gamma). IBMX increases cAMP content, which can activate kinase A (PKA) and/or EPAC (exchange activated cAMP). To investigate importance differentiation mixture, we first...
In Buenos Aires periurban area, horticultural practices are one of the most important activities.Pesticides and fertilisers used without any control to cover farmers' needs, obtaining high crop yields at short terms modifying soil ecosystem in long term.The aim this work was isolate indigenous strains from units with pesticide degrading capacity evaluate their plant growth-promoting properties order design biofertilisers be applied restoration these exploited soils.After screening, eight...
Abstract Polyhydroxyalkanoates (PHAs) are intracellular polymers that enhance bacterial fitness against various environmental stressors. Pseudomonas extremaustralis 14-3b is an Antarctic bacterium capable of accumulating, short-chain-length PHAs (sclPHAs), composed C3–C5 monomers, as well medium-chain-length (mclPHAs) containing ≥C6 monomers. Since pH changes pivotal in physiology, influencing microbial growth and metabolic processes, we propose accumulated PHA increases P. to cope with...
Oil contamination is an environmental issue worldwide, and bioremediation has emerged as a preferred strategy to address this challenge. One of the limiting factors in oil biodegradation its bioavailability, which can be solved by addition tensioactive compounds. Given increasing demand for environmentally friendly surfactants, study aimed obtain cell-free biosurfactant extracts additives surfactant enhanced remediation (SER) protocols. Diesel-degrading, surfactant-producing strains were...
Industrial Biotechnology and Applied Microbiology is an optional 128h-course for Chemistry Biology students at the Faculty of Sciences, University Buenos Aires, Argentina. This course usually attended by 25 students, working in teams two. The curriculum, with 8 lab exercises, includes oil bioremediation practice covering insight processes: influence pollutants on autochthonous microbiota, biodegrader isolation biosurfactant production bioavailability understanding. experimental steps are:...