A5079828513- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Plant Molecular Biology Research
- Parasitic Infections and Diagnostics
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Circadian rhythm and melatonin
- Radioactive contamination and transfer
- Biochemical and Molecular Research
- Nuclear and radioactivity studies
- Synthesis and Biological Evaluation
- Insect symbiosis and bacterial influences
- Viral Infections and Immunology Research
- Helminth infection and control
- Birth, Development, and Health
- Circular RNAs in diseases
- HIV/AIDS Research and Interventions
- Cancer-related molecular mechanisms research
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Osteoarthritis Treatment and Mechanisms
- FOXO transcription factor regulation
- Dietary Effects on Health
- Plant Gene Expression Analysis
- Lipid metabolism and biosynthesis
Scripps (United States)
2020-2022
Scripps Institution of Oceanography
2020-2022
Scripps Research Institute
2018-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2021
National Agricultural Technology Institute
2012-2021
AstraZeneca (Sweden)
2021
Instituto de Agrobiotecnología del Litoral
2021
University of Southern California
2018-2020
National Institute of Industrial Technology
2014
Experimental Medicine and Biology Institute
2005-2013
Background A century after its discovery, Chagas disease still represents a major neglected tropical threat. Accurate diagnostics tools as well surrogate markers of parasitological response to treatment are research priorities in the field. The purpose this study was evaluate performance PCR methods detection Trypanosoma cruzi DNA by an external quality evaluation. Methodology/Findings An international collaborative launched expert laboratories from 16 countries. Currently used strategies...
This report describes a real-time PCR (Q-PCR) strategy to quantify Trypanosoma cruzi (T. cruzi) DNA in peripheral blood samples from Chagas disease patients targeted conserved motifs within the repetitive satellite sequence.The Q-PCR has detection limit of 0.1 and 0.01 parasites/mL, with dynamic range 10(6) 10(7) for Silvio X10 cl1 I) Cl Brener stocks IIe), respectively, an efficiency 99%, coefficient determination (R(2)) 0.998. In order express accurately parasitic loads: (1) we adapted...
Background The analytical validation of sensitive, accurate and standardized Real-Time PCR methods for Trypanosoma cruzi quantification is crucial to provide a reliable laboratory tool diagnosis recent infections as well monitoring treatment efficacy. Methods/Principal Findings We have validated multiplex quantitative assay (qPCR) based on TaqMan technology, aiming quantify T. satellite DNA an internal amplification control (IAC) in single-tube reaction. IAC allows rule out false negative...
One hundred years after the discovery of Chagas disease, it remains a major neglected tropical disease. Chronic heart disease (cChHD) is most severe manifestation. Heart transplantation proper treatment for end-stage failure, although reactivation may result receipt immunosuppressive therapy. T. cruzi strains cluster into 6 discrete typing units (DTUs; I-VI) associated with different geographical distribution, transmission cycles and varying symptoms. In southern cone South America, II, V,...
Objectives Single-cell level analysis of articular cartilage and meniscus tissues from human healthy osteoarthritis (OA) knees. Methods RNA sequencing (scRNA-seq) analyses were performed on (n=6, n=7) OA n=6) Expression genes interest was validated using immunohistochemistry RNA-seq function analysed by gene overexpression depletion. Results scRNA-seq knee (70 972 cells) (78 017 identified a pathogenic subset that is shared between both tissues. This cell population expanded in has strong...
Identifying the target genes of transcription factors is important for unraveling regulatory networks in all types organisms. Our interest was precisely to uncover spectrum loci regulated by a widespread plant factor involved physiological adaptation drought, type stress that plants have encountered since colonization land habitats 400 MYA. The regulator under study, named ASR1, exclusive kingdom (albeit absent Arabidopsis) and known alleviate caused restricted water availability. As its are...
Background Trypanosoma cruzi has been classified into six Discrete Typing Units (DTUs), designated as TcI–TcVI. In order to effectively use this standardized nomenclature, a reproducible genotyping strategy is imperative. Several typing schemes have developed with variable levels of complexity, selectivity and analytical sensitivity. Most them can be only applied cultured stocks. context, we aimed develop multiplex Real-Time PCR method identify the T. DTUs using TaqMan probes (MTq-PCR)....
Either expression level or transcriptional activity of various nuclear receptors (NRs) have been demonstrated to be under circadian control. With a few exceptions, little is known about the roles NRs as direct regulators circuitry. Here we show that receptor HNF4A strongly transrepresses CLOCK:BMAL1 heterodimer. We define central role for in maintaining cell-autonomous oscillations tissue-specific manner liver and colon cells. Not only transcript but also genome-wide chromosome binding...
Trypanosoma cruzi DNA was amplified from an intracranial biopsy and peripheral blood of HIV patient with encephalitis; this episode indicative AIDS congenital Chagas disease. The analysis a micro-satellite locus revealed multiclonal parasite population at the brain lesion more complex minicircle signature than that profiled in using restriction fragment length polymorphism (RFLP)-PCR low stringency single primer (LSSP) PCR. Interestingly, different sublineages T. II were detected by means...
Trypanosoma cruzi lineages, microsatellite allelic polymorphism, and mithocondrial gene haplotypes were directly typified from peripheral blood cerebrospinal fluid specimens of a Bolivian patient with Chagas disease accompanying AIDS central nervous system severe involvement. Of note, the patient's was infected by mixture T. I IId/e polyclonal populations while showed only monoclonal population. Our findings do not corroborate original assumption innocuity for in southern cone Americas...
SUMMARY Genetic diversity of Trypanosoma cruzi may play a role in pathogenesis Chagas disease forms. Natural populations are classified into 6 Discrete Typing Units (DTUs) Tc I-VI with taxonomical status. This study aimed to identify T. DTUs bloodstream and tissue samples Argentinean patients disease. PCR-based strategies allowed DTU identification 256 clinical from 239 patients. V prevailed blood both asymptomatic symptomatic cases I was more frequent bloodstream, cardiac tissues chagoma...
This study applied improved DNA extraction and polymerase chain reaction strategies for screening identification of Trypanosoma cruzi lineages directly from faeces triatomines collected in a well-defined rural area northwestern Argentina. Amplification the variable regions kinetoplastid minicircle genome (kDNA-PCR) was performed faecal lysates 33 microscope (MO)-positive 93 MO-negative Triatoma infestans , 2 MO-positive 38 guasayana 73 garciabesi . kDNA-PCR detected T. 91% 7·5% which were...
Targeted protein degradation (TPD) is an emerging therapeutic modality in which small molecules are used to recruit targets the natural machinery of cell. Molecular glue degraders (MGD) monovalent that accomplish this by redirecting E3 ubiquitin ligases target proteins, offering potential degrade previously unliganded and ″undruggable″ proteins cancer, neurodegenerative, other diseases. While attractive due their drug-like properties, MGDs exceptionally hard discover have largely been...
Objective Osteoarthritis ( OA ) is the most common age‐related joint disease. With aging and in , expression of FoxO transcription factors reduced, diminishing their chondroprotective actions. In order to elucidate molecular mechanisms by which FoxO1 protects chondrocytes, we sought identify genome‐wide occupancy profile FoxO1. Methods We performed chromatin immunoprecipitation followed high‐throughput sequencing (Ch IP ‐Seq) on human primary chondrocytes. Ch ‐Seq data were integrated with...
Trypanosoma cruzi infection of host cells is a complex process in which many proteins participate but only few these have been identified experimentally. One parasite factor likely to be involved the protein product LYT1, single-copy gene cloned, sequenced, and characterized by Manning-Cela et al. (Infect. Immun. 69:3916-3923, 2001). This was potentially associated with infectivity, since deletion both LYT1 alleles CL Brenner strain (the wild type [WT]) resulted null mutant T. clone (L16)...