A5027809373- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Reproductive Biology
- Antioxidant Activity and Oxidative Stress
- Plant Gene Expression Analysis
- Plant Disease Resistance and Genetics
- Sugarcane Cultivation and Processing
- Plant nutrient uptake and metabolism
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Biofuel production and bioconversion
- Postharvest Quality and Shelf Life Management
- Plant Physiology and Cultivation Studies
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Microbial Metabolites in Food Biotechnology
- Plant Micronutrient Interactions and Effects
- Lipid metabolism and biosynthesis
- Nematode management and characterization studies
Instituto de Botânica
2015-2024
Universidade de São Paulo
2015-2024
Instituto de Geociencias
2023
Universidade Cidade de São Paulo
2015
Instituto de Tecnologia de Alimentos
2011
National Agricultural Technology Institute
1994-2011
Instituto Nacional de Tecnologia
2011
Cornell University
2010
National Institute of Industrial Technology
2010
Consejo Nacional de Investigaciones Científicas y Técnicas
2010
Resistance against the aphid Macrosiphum euphorbiae previously was observed in tomato and attributed to a novel gene, designated Meu-1 , tightly linked nematode resistance Mi. Recent cloning of Mi allowed us determine whether are same gene. We show that is expressed leaves, isolate-specific, susceptible transformed with resistant isolates as original lines. conclude gene mediates both aphids nematodes, organisms belonging different phyla. first example plant active two such distantly related...
Light signaling and plant hormones, particularly ethylene auxins, have been identified as important regulators of carotenoid biosynthesis during tomato fruit ripening. However, whether how the light hormonal cascades crosstalk to control this metabolic route remain poorly elucidated. Here, potential involvement auxins in light-mediated regulation carotenogenesis was investigated by comparing impacts treatments light-hyperresponsive high pigment-2 (hp2) mutation on both synthesis signaling....
Abstract Chlorophyll breakdown occurs in different green plant tissues (e.g. during leaf senescence and ripening fruits). For species, the PHEOPHORBIDE A OXYGENASE (PAO)/phyllobilin pathway has been described to be major chlorophyll catabolic pathway. In this pathway, pheophorbide (i.e. magnesium- phytol-free chlorophyll) as a core intermediate. Most of enzymes involved PAO/phyllobilin are known; however, mechanism dephytylation remains uncertain. During Arabidopsis (Arabidopsis thaliana)...
Nitric oxide (NO) has been implicated as part of the ripening regulatory network in fleshy fruits. However, very little is known about simultaneous action NO on events and metabolic reactions behind ripening-related changes fruit color, taste, aroma nutritional value. Here, we performed an in-depth characterization concomitant tomato (Solanum lycopersicum) transcriptome metabolome associated with delayed-ripening phenotype caused by supplementation at pre-climacteric stage. Approximately...
Senescence is the process that marks end of a leaf's lifespan. As it progresses, massive macromolecular catabolism dismantles chloroplasts and, consequently, decreases photosynthetic capacity these organs. Thus, senescence manipulation strategy to improve plant yield by extending photosynthetically active window time. However, remains be addressed if this approach can fleshy fruit production and nutritional quality. One way delay initiation regulating key transcription factors (TFs) involved...
In a changing environment, plants need to cope with the impact of rising temperatures together high light intensity. Here, we used lipidomics in tomato model system identify lipophilic molecules that enhance tolerance combined high-temperature and high-light stress. Among several hundred metabolites, two most strongly up-regulated compounds were α-tocopherol plastoquinone/plastoquinol. Both are well-known lipid antioxidants contribute protection photosystem II (PSII) against photodamage...
Vegetables are critical for human health as they a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis VTE compounds, tocopherol and tocotrienol, derives from precursors shikimate methylerythritol phosphate pathways. Quantitative trait loci (QTL) α-tocopherol content in ripe fruit have previously been determined an Solanum pennellii tomato introgression line population. this work, variations isoforms (α, β, γ, δ) fruits these lines were studied. parallel all genes...
The transition from etiolated to green seedlings involves the conversion of etioplasts into mature chloroplasts via a multifaceted, light-driven process comprising multiple, tightly coordinated signaling networks. Here, we demonstrate that light-induced greening and chloroplast differentiation in tomato (Solanum lycopersicum) are mediated by an intricate cross talk among phytochromes, nitric oxide (NO), ethylene, auxins. Genetic pharmacological evidence indicated either endogenously produced...
Although the importance of light for tomato plant yield and edible fruit quality is well known, PHYTOCHROME INTERACTING FACTORS (PIFs), main components phytochrome-mediated signal transduction, have been studied almost exclusively in Arabidopsis thaliana. Here, diversity, evolution expression profile PIF gene subfamily Solanum lycopersicum was characterized. Eight loci were identified, named SlPIF1a, SlPIF1b, SlPIF3, SlPIF4, SlPIF7a, SlPIF7b, SlPIF8a SlPIF8b. The duplication SlPIF1, SlPIF7...
Light signaling has long been reported to influence fruit biology, although the regulatory impact of fruit-localized photoreceptors on development and metabolism remains unclear. Studies performed in phytochrome (PHY)-deficient tomato (Solanum lycopersicum) mutants suggest that SlPHYA, SlPHYB2, a lesser extent SlPHYB1 ripening. By employing fruit-specific RNAi-mediated silencing SlPHY genes, we demonstrated SlPHYA SlPHYB2 play contrasting roles regulating plastid biogenesis maturation...
Tocopherols are important antioxidants exclusively produced in plastids that protect the photosynthetic apparatus from oxidative stress. These compounds with vitamin E activity also essential dietary nutrients for humans. Although tocopherol biosynthetic pathway has been elucidated, mechanisms regulate production and accumulation remain elusive. Here, we investigated regulatory mechanism underlying biosynthesis during ripening tomato fruits, which an source of E. Our results show under light...
Shade-intolerant plants rapidly elongate their stems, branches, and leaf stalks to compete with neighboring vegetation, maximizing sunlight capture for photosynthesis. This rapid growth adaptation, known as the shade-avoidance response (SAR), comes at a cost: reduced biomass, crop yield, root growth. Significant progress has been made on mechanistic understanding of hypocotyl elongation during SAR; however, molecular interpretation repression is not well understood. Here, we explore...
During the last decade, knowledge about BBX proteins has greatly increased. Genome-wide studies identified gene family in several ornamental, industry, and food crops; however, reports regarding role of these genes as regulators agronomically important traits are scarce. Here, by phenotyping a knockout mutant, we performed comprehensive functional characterization tomato locus Solyc12g089240, hereafter called SlBBX20. The data revealed encoded protein positive regulator light signaling...
Abstract To identify genomic regions involved in the regulation of fundamental physiological processes such as photosynthesis and respiration, a population Solanum pennellii introgression lines was analyzed. We determined phenotypes for physiological, metabolic, growth related traits, including gas exchange chlorophyll fluorescence parameters. Data analysis allowed identification 208 metabolic quantitative trait loci with 33 these being associated to smaller intervals regions, termed BINs....
Plant development is highly dependent on the ability to perceive and cope with environmental changes. In this context, PIF proteins are key players in cellular hub controlling responses fluctuating light temperature conditions. Reports various plant species show that manipulation of PIF4 level affects important agronomical traits. tomato (Solanum lycopersicum), SlPIF1a SlPIF3 regulate fruit nutraceutical composition. However, wider role protein family, potential their for improvement other...
Plastids are organelles responsible for essential aspects of plant development, including carbon fixation and synthesis several secondary metabolites. Chloroplast differentiation activity highly regulated by light, proteins involved in these processes have been characterised. Such is the case GOLDEN 2-LIKE (GLK) transcription factors, which induces expression genes related to chloroplast photosynthesis. The tomato (Solanum lycopersicum) genome harbours two copies this gene, SlGLK1 SlGLK2,...
Photoreceptor engineering has recently emerged as a means for improving agronomically beneficial traits in crop species. Despite the central role played by red/far-red photoreceptor phytochromes (PHYs) controlling fruit physiology, applicability of PHY increasing fleshy nutritional content remains poorly exploited. In this study, we demonstrated that fruit-specific overexpression constitutively active GAF domain Tyr
Summary Transposable elements (TEs) are considered to be important components of the maintenance and diversification genomes. The recent increase in genome sequence data has created an opportunity evaluate impact these active mobile on evolution plant Analysis sugarcane transcriptome identified 267 clones with significant similarity previously described TEs. After full cDNA sequencing, 68 TE were assigned 11 families according their best alignment against a fully characterized element....