A5032026453- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Hepatitis B Virus Studies
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Clostridium difficile and Clostridium perfringens research
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Urinary Tract Infections Management
- Toxoplasma gondii Research Studies
- Otolaryngology and Infectious Diseases
- Salmonella and Campylobacter epidemiology
- vaccines and immunoinformatics approaches
- Pharmaceutical and Antibiotic Environmental Impacts
- Inflammasome and immune disorders
The University of Sydney
2018-2025
Westmead Institute for Medical Research
2018-2025
Westmead Hospital
2018-2021
University of the Sunshine Coast
2019
The University of Queensland
2011-2016
Westmead Institute
2016
University of Wollongong
2009
Abstract The group A Streptococcus (GAS) M1T1 clone emerged in the 1980s as a leading cause of epidemic invasive infections worldwide, including necrotizing fasciitis and toxic shock syndrome 1,2,3 . Horizontal transfer mobile genetic elements has played central role evolution 4,5 , with bacteriophage-encoded determinants DNase Sda1 6 superantigen SpeA2 7 contributing to enhanced virulence colonization respectively. Outbreaks scarlet fever Hong Kong China 2011, caused primarily by emm 12 GAS...
Enterohemorrhagic Escherichia coli (EHEC) strains are zoonotic pathogens responsible for a range of severe human disease. The repertoire virulence determinants promoting EHEC disease is encoded on both the main chromosome and plasmid. We examined multiply antibiotic-resistant O26 strain carriage resistance genes plasmid containing complex antibiotic-resistance gene locus, designated as pO26-CRL, was purified from O26:H− (patient with hemorrhagic colitis) subjected to shotgun-sequencing...
ABSTRACT Between June and November 2010, a concerning rise in the number of cases puerperal sepsis, postpartum pelvic bacterial infection contracted by women after childbirth, was observed New South Wales, Australia, hospital system. Group A streptococcus (GAS; Streptococcus pyogenes ) isolates PS001 to PS011 were recovered from nine patients. Pulsed-field gel electrophoresis emm sequence typing revealed that GAS emm1.40 , emm75.0 emm77.0 emm89.0 emm89.9 each single patient, ruling out...
Enterohemorrhagic Escherichia coli (EHEC) and atypical enteropathogenic E. (aEPEC) are important zoonotic pathogens that increasingly becoming resistant to multiple antibiotics. Here we describe two plasmids, pO26-CRL125 (125 kb) from a human O26:H- EHEC, pO111-CRL115 (115kb) bovine O111 aEPEC, impart resistance ampicillin, kanamycin, neomycin, streptomycin, sulfathiazole, trimethoprim tetracycline both contain class 1 integrons with an identical IS26-mediated deletion in their 3´-conserved...
Abstract The spread of multidrug resistance via mobile genetic elements is a major clinical and veterinary concern. Pathogenic Escherichia coli harbour antibiotic virulence genes mainly on plasmids, but also bacteriophages hybrid phage-like plasmids. In this study, the genomes three E. pJIE250-3 from human isolate, pSvP1 porcine ETEC O157 pTZ20_1P commensal , were sequenced (PacBio RSII), annotated compared. All are coliphage P1 variants, each with unique adaptations. P1-derivative that has...
The past 50 years has witnessed the emergence of new viral and bacterial pathogens with global effect on human health. hyperinvasive group A Streptococcus (GAS) M1T1 clone, first detected in mid-1980s United States, since disseminated worldwide remains a major cause severe invasive infections. Although much is understood regarding capacity this pathogen to disease, less known precise evolutionary events selecting for its emergence. We used high-throughput technologies sequence World Health...
Abstract Vibrio species are an emerging public and animal health risk in marine environments the opportunistic bacterial pathogen harveyi is a major disease for tropical aquaculture. Current understanding of virulence V. limited by strain-specific variability complex host-pathogen dynamics. This study sought to integrate genomic investigation, phenotypic characterisation vivo challenge trials barramundi ( Lates calcarifer) increase our virulence. We identified two hypervirulent isolates,...
ABSTRACT Bacteriophages (phages) are emerging as a viable adjunct to antibiotics for the treatment of multidrug‐resistant (MDR) bacterial infections. While intravenous phage therapy has proven successful in many cases, clinical outcomes remain uncertain due limited understanding host response phages. In this study, we conducted comprehensive examination interaction between clinical‐grade phages used treat MDR Escherichia coli and Klebsiella pneumoniae infections, human peripheral blood...
Bacteriophages (phages) are estimated to be the most abundant microorganisms on Earth. Their presence in human blood suggests that they can translocate from non-sterile sites such as gastrointestinal tract where concentrated. To examine phage translocation ex vivo, we adapted a primary colonoid monolayer model possessing cell diversity and architecture, thick layer of mucus akin colonic environment vivo. We show is superior Caco-2 cell-line model, intact organized tight junctions generating...
A serological survey was conducted in the Patagonia region of Argentina to estimate prevalence nine disease agents within populations free-ranging culpeo (Dusicyon culpaeus) and grey griseus) foxes. The were Aujeszky's virus (ADV), Brucella, canine adenovirus (CAV), distemper (CDV), parvovirus (CPV), Encephalitozoon cuniculi, Leptospira, Neospora caninum, Toxoplasma gondii. total 84 foxes sampled (28 56 grey), 73% sera had antibodies against one or more pathogens. Among these seropositive...
Abstract Postpartum women are at increased risk of developing puerperal sepsis caused by group A Streptococcus (GAS). Specific GAS serotypes, including M1 and M28, more commonly associated with sepsis. However, the mechanisms genital tract infection not well understood. We utilized a murine carriage model to demonstrate that M28 colonization triggers TNF-α, IL-1β, IL-17A production in female tract. GAS-induced significantly influences streptococcal alters local inflammatory responses two...
Multidrug resistant (MDR) carbapenemase-producing (CP) Klebsiella pneumoniae, belonging to clonal group CG258, is capable of causing severe disease in humans and classified as an urgent threat by health agencies worldwide. Bacteriophages are being actively explored therapeutic alternatives antibiotics. In effort define a robust experimental approach for effective selection lytic viruses therapy, we have fully characterized the genomes 18 Kumoniae target strains tested them against novel...
Most diseases in aquaculture are caused by opportunistic pathogens. One of them, Vibrio harveyi, is a widespread Gram-negative bacterium that has become an important pathogen aquatic species marine environments. Here, we propose the use causal pie model as framework to conceptualize causation vibriosis juvenile barramundi (Lates calcarifer) and establish effective challenge model. In model, sufficient cause, or pie, constellation component causes lead outcome (e.g. vibriosis). pilot study,...
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. (EPEC) are diarrheagenic pathotypes of that cause gastrointestinal disease with the potential for life-threatening sequelae. While certain EHEC EPEC virulence mechanisms have been extensively studied, factors mediate host colonisation remain to be properly defined. Previously, we identified four genes (ehaA, ehaB, ehaC ehaD) from prototypic strain EDL933 encode proteins belong autotransporter (AT) family. Here examined...
ABSTRACT The resurgence of invasive disease caused by Streptococcus pyogenes (group A [GAS]) in the past 30 years has paralleled emergence and global dissemination highly virulent M1T1 clone. GAS clone diverged from ancestral M1 serotype horizontal acquisition two unique bacteriophages, encoding potent DNase Sda1/SdaD2 superantigen SpeA, respectively. phage-encoded promotes escape neutrophil extracellular traps is linked to enhanced virulence In this study, we successfully used vitro...
Sequence type (ST)73 has emerged as one of the most frequently isolated extraintestinal pathogenic Escherichia coli . To examine localized diversity ST73 clonal groups, including their mobile genetic element profile, we sequenced genomes 16 multiple-drug resistant isolates from patients with urinary tract infection a single hospital in Sydney, Australia, between 2009 and 2011. Genome sequences were used to generate SNP-based phylogenetic tree determine relationship these global context ( n...
Effective antimicrobial stewardship requires a better understanding of the impact different antibiotics on gut microflora. Studies with humans are confounded by large interindividual variability and difficulty in identifying control cohorts. However, controlled murine models can provide valuable information. In this study, we examined penicillin-like antibiotic (piperacillin-tazobactam [TZP]) or third-generation cephalosporin (ceftriaxone [CRO]) microbiota analysis changes fecal microbiome...