A5067999368- Virus-based gene therapy research
- Respiratory viral infections research
- Nitric Oxide and Endothelin Effects
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Asthma and respiratory diseases
- interferon and immune responses
- Viral Infections and Immunology Research
- Malaria Research and Control
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Endometriosis Research and Treatment
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Parvovirus B19 Infection Studies
- HIV/AIDS drug development and treatment
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- vaccines and immunoinformatics approaches
- Cancer Research and Treatments
University of Michigan
2015-2024
C. S. Mott Children's Hospital
2023
Pediatrics and Genetics
2022
Duke Medical Center
2001-2021
National Center for Emerging and Zoonotic Infectious Diseases
2021
Centers for Disease Control and Prevention
2021
Duke University
1996-2019
Durham VA Medical Center
1987-2019
Michigan United
2013-2015
Regional West Medical Center
2008-2014
Human immunodeficiency virus type 1 (HIV-1) infection of T lymphocytes requires cellular proliferation and DNA synthesis. monocytes were shown to have low synthesis rates, yet the monocytotropic BaL isolate HIV-1 was able infect these cells efficiently. Monocytes that irradiated assure no could also be readily infected with HIV-1BaL. Such infections associated integration HIV-1BaL into high molecular weight, chromosomal monocytes. Thus, normal, nonproliferating differ from in a productive...
Tumor killing by activated macrophages is not a highly determined biologic event, but relative capability influenced the local environment. An intrinsic macrophage cytotoxic effector system modulated serum and other environmental factors that can either enhance or suppress tumor killing. Activated kill cells only when regulating threshold drops to critically low level.
Abstract These studies demonstrate the potent effect of LPS on induction macrophage-mediated tumor cell killing and show, by using pure populations cloned macrophages as effector cells, that can act in complete absence contaminating lymphocytes. Macrophage differentiation toward tumoricidal state parallels responsiveness to LPS. Normal will not kill even presence 10 µg/ml LPS; peptone-induced normal (stimulated macrophages) are made ≥500 mg/ml nontumoricidal BCG-activated cells 0.5 1.0 ng/ml...
Abstract Nitric oxide (NO) production and NO synthase (NOS) expression are increased in osteoarthritis rheumatoid arthritis, suggesting that may play a role the destruction of articular cartilage. To test hypothesis mechanical stress increase by chondrocytes, we measured effects physiological levels static intermittent compression on NOS activity, production, antigen porcine cartilage explants. Static significantly at 0.1 MPa for 24 h ( P < 0.05). Intermittent 0.5 Hz 6 followed 18...
Human alveolar macrophages (HAM) from 28 normal volunteers were found to inhibit replication of Cryptococcus neoformans. Conditions under which fungistasis occurred different than those required for mouse peritoneal macrophage-mediated fungistasis. Inhibition fungal by (MPM) requires that the are activated and cocultures C. neoformans be done in presence serum, L-arginine, endotoxin. During MPM-mediated tumor cell killing, L-arginine is oxidized NO2−, NO3−, L-citrulline. In addition, MPM...
Monocyte or macrophage polykaryons (MP) are seen in different tissues various inflammatory states and normal bone (osteoclasts). The factors controlling the formation function of MP not completely understood. This study was designed to evaluate effects lymphokine gamma-interferon (IFN-gamma) on human monocyte vitro. Purified recombinant IFN-gamma [20-200 units/ml (0.1-1.0 nM)] caused appearance cultures monocytes cultured 10% unheated autologous serum. were noted by as early 36 hr culture...
Abstract Dichlorofluorescein (DCFH) oxidation assay measures hydrogen peroxide (H2O2), which is a derivative of superoxide anion. We found that calmodulin antagonist, W-13, known to inhibit anion generation enhanced the capacity human neutrophils oxidize DCFH. To investigate this discrepancy we studied role nitric oxide (NO) in DCFH oxidation. Pure NO was capable oxidizing DCFH, and product formed had spectral properties identical oxidized produced by H2O2. The arginine analog,...
The cytokine gamma interferon (IFN-gamma) is critical for resistance to Toxoplasma gondii. IFN-gamma strongly activates macrophages and nonphagocytic host cells limit intracellular growth of T. gondii; however, the cellular factors that are required this effect largely unknown. We have shown previously IGTP LRG-47, members IFN-gamma-regulated family p47 GTPases, acute gondii infections in vivo. In contrast, IRG-47, another member family, not required. present work, we addressed whether these...
Rationale: Idiopathic pulmonary fibrosis is a progressive disease with high mortality. Although most patients have slow, course, some will an acute deterioration in function or exacerbation, which carries poor prognosis. In cases, associated infection. Herpesviruses been this disease. Fibrocytes also shown to be important the pathogenesis of fibrosis.Objectives: To develop murine model for infectious exacerbation preexisting fibrosis, and provide mechanistic insight into role herpesviruses...
Abstract It has recently been shown that tumor necrosis factor (TNF) induces increased la antigen expression on a malignant murine macrophage cell line, and TNF is synergistic with gamma interferon (IFN) in inducing expression. This finding raises the possibility serves as non-interferon activating vivo. Since it known different effects benign cells, we chose to evaluate of recombinant primary cultures peritoneal macrophages (MP). Neither human nor proportion MP which expressed antigen,...
The tumor promoting phorbol diesters elicit a variety of responses from normal and leukemic blood cells in vitro by apparently interacting with cellular receptors. biologically active ligand [20-(3)H] 12,13-dibutyrate ([(3)H]PDBu) bound specifically to intact human lymphocytes, monocytes, polymorphonuclear leukocytes (PMN), platelets, but not erythrocytes. Binding, which was comparable for all four cell types, occurred rapidly at 23 degrees 37 C, reaching maximum 20-30 min usually followed...
Abstract Recent studies link early rhinovirus (RV) infections to later asthma development. We hypothesized that neonatal RV infection leads an IL-13–driven asthma-like phenotype in mice. BALB/c mice were inoculated with RV1B or sham on day 7 of life. Viral RNA persisted the lung up d postinfection. Within this time frame, IFN-α, -β, and -γ peaked 1 postinfection, whereas IFN-λ levels persisted. Next, we examined 35 life, 28 after initial infection. Compared sham-treated controls,...
Summary Priapism occurs in 30–45% of male patients with sickle cell disease (SCD), but the possible influence genetic risk factors on incidence priapism is not well understood. We examined polymorphisms 199 unrelated, adult (>18 years), Hb SS and S β 0 ‐thalassaemia, 83 (42%) whom reported a history priapism. Candidate genes for association were identified based their involvement adhesion, coagulation, inflammation signalling. Additionally, we involved nitric oxide biology ( NOS2, NOS3,...