A5073294634- Ocular Surface and Contact Lens
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
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- Glaucoma and retinal disorders
- Antimicrobial Peptides and Activities
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University of Virginia
2016-2025
Ophthalmology Associates (United States)
2022
Princess Alexandra Hospital
2020
Institute of Cell Biology
2005-2013
University of Virginia Health System
2008-2013
James Madison University
2008
The Ohio State University
2008
Kanazawa Medical University
2008
National Institutes of Health
1985-2007
University of Toronto
1996
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBasement membrane complexes with biological activityHynda K. Kleinman, Mary L. McGarvey, John R. Hassell, Vicki Star, Frances B. Cannon, Gordon W. Laurie, and George MartinCite this: Biochemistry 1986, 25, 2, 312–318Publication Date (Print):January 28, 1986Publication History Published online1 May 2002Published inissue 28 January 1986https://doi.org/10.1021/bi00350a005RIGHTS & PERMISSIONSArticle Views2265Altmetric-Citations1064LEARN ABOUT THESE...
Electron microscopic immunostaining of rat duodenum and incisor tooth was used to examine the location four known components basement-membrane region: type IV collagen, laminin, heparan sulfate proteoglycan, fibronectin. Antibodies or antisera against these substances were localized by direct indirect peroxidase methods on 60-microns thick slices formaldehyde-fixed tissues. In region duodenal epithelium, enamel-organ blood-vessel endothelium, for all observed in basal lamina (also called...
Abstract Electron microscopic immunostaining was used to examine the localization of type IV collagen, laminin, entactin, heparan sulfate proteoglycan, and fibronectin within basement membranes rat kidney. In preliminary experiments, various methods processing formaldehyde‐fixed kidney were compared using antilaminin antiserum indirect immunoperoxidase method. Little or no laminin glomerular membrane present in sections unless they had been frozen‐thawed; even this case, light comparison...
The ultrastructure of Reichert's membrane, a thick basement membrane in the parietal wall yolk sac, has been examined 13-14-d pregnant rats. This is composed more or less distinct parallel layers, each one which resembles common membrane. After routine fixation glutaraldehyde followed by osmium tetroxide, layers appear to be mainly 3-8-nm cords arranged three-dimensional network. Loosely scattered among are unbranched, straight tubular structures with diameter 7-10 nm, run surface and...
The appearance of extracellular matrix molecules and their receptors represent key events in the differentiation cells kidney. Steady-state mRNA levels for a laminin receptor, B1, B2, A chains, alpha 1-chain collagen IV (alpha 1[IV]), were examined mouse kidneys at 16 d gestation birth, when cell is active, 1-3 wk after birth this activity has subsided. Northern analysis revealed that expression receptor precedes 1(IV) B chains whereas chain was very low. In situ hybridization reflected...
Abstract Immunohistochemical methods were used to determine whether type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan present in diverse basement membranes. Antisera or antibodies against each substance prepared, tested by enzyme‐linked immunosorbent assay, exposed frozen sections of duodenum, trachea, kidney, spinal cord, cerebrum, incisor tooth from rats aged 20 days 34 months. Bound then localized indirect direct peroxidase for examination the light microscope....
Lacritin is a tear glycoprotein with prosecretory, prosurvival, and mitogenic properties. We examined lacritin levels in the tears of Sjögren's syndrome (SS) patients explored therapeutic potential topical for treatment keratoconjunctivitis sicca.Tears from healthy controls (n = 14) SS 15) were assayed using C-terminal antibody. In paired-eye study, autoimmune regulator (Aire) knockout (KO) mice 7) treated three times daily 21 days 10 μL 4 μM (left eye) or vehicle (PBS) control (right eye)....
Autophagy is a catabolic self-degradative pathway that promotes the degradation and recycling of intracellular material through lysosomal compartment. Although first believed to function in conditions nutritional stress, autophagy emerging as critical cellular pathway, involved variety physiological pathophysiological processes. dysregulation associated with an increasing number diseases, including ocular diseases. On one hand, mutations autophagy-related genes have been linked cataracts,...
Basement membrane-adherent type II alveolar cells isolated from lung assemble into lumen-containing cellular spheres which retain the correct polarity and thereby approximate earliest fetal stage of morphogenesis. The molecular basis this process, determined in initial experiments to be attributable mainly large heterotrimeric glycoprotein laminin, was probed with laminin proteolytic fragments, antibodies, synthetic peptides. carboxy-terminal fragment E8, but not equimolar amounts P1,...
Antibodies to type IV collagen were linked with peroxidase and used for direct immunostaining of Reichert's membrane the associated cells rat parietal yolk sac. Immunostaining was observed throughout thickness within endodermal arranged as a single layer on its inner side. The mainly present in cisternae rough endoplasmic reticulum (rER) Golgi apparatus, where it could occur any saccule, but predominated GERL elements prosecretory granule-like structures. Moreover, secretory structures...
Lacritin is a novel human tear glycoprotein that promotes basal peroxidase secretion by rat lacrimal acinar cells in vitro. This study investigates whether lacritin prosecretory when added topically to the ocular surface of normal living rabbits, and if so, what its efficacy tolerability versus cyclosporine artificial tears.Purified recombinant (1, 10, 50, or 100 μg/mL), inactive truncation mutant C-25 (10 (0.05%), tears were administered eyes New Zealand White rabbits either as single dose...
Discovering new bacterial signaling pathways offers unique antibiotic strategies. With current classes targeting cell wall synthesis or depolarizing the inner-membrane altering metabolome inhibiting replication transcription pathways, manipulation of transporters to limit respiration and thereby pathogenesis has been a decades long quest. Here we report an inhibitor multiple transporters. The is bactericidal N-104 endogenous cleavage fragment prosecretory mitogen lacritin. Lacritin now known...
Renewal of nongermative epithelia is poorly understood. The novel mitogen “lacritin” apically secreted by several epithelia. We tested 17 different cell types and discovered that lacritin preferentially mitogenic or prosecretory for those normally contact during its glandular outward flow. Mitogenesis dependent on lacritin's C-terminal domain, which can form an α-helix with a hydrophobic face, as per VEGF's PTHLP's respective dimerization receptor-binding domain. Lacritin targets downstream...
Cell surface heparan sulfate (HS) proteoglycans are carbohydrate-rich regulators of cell migratory, mitogenic, secretory, and inflammatory activity that bind present soluble heparin-binding growth factors (e.g., fibroblast factor, Wnt, Hh, transforming factor β, amphiregulin, hepatocyte factor) to their respective signaling receptors. We demonstrate the deglycanated core protein syndecan-1 (SDC1) not HS chains nor SDC2 or -4, appears target epithelial selective prosecretory mitogen lacritin....