A5059857634- Ocular Surface and Contact Lens
- Salivary Gland Disorders and Functions
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Nasolacrimal Duct Obstruction Treatments
- Glaucoma and retinal disorders
- Ophthalmology and Eye Disorders
- Connective tissue disorders research
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Corneal Surgery and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Cancer Treatment and Pharmacology
- Galectins and Cancer Biology
- Adenosine and Purinergic Signaling
- Diabetes and associated disorders
- Photosynthetic Processes and Mechanisms
- Light effects on plants
University of Southern California
2016-2025
Southern California Eye Institute
2017-2025
Keck Hospital of USC
2020-2024
Southern California University for Professional Studies
1996-2024
Baylor College of Medicine
2024
LAC+USC Medical Center
2003-2020
GTx (United States)
2019-2020
Southern California Clinical and Translational Science Institute
2015
Institute of Cell Biology and Neurobiology
2006-2011
Pharmaceutical Biotechnology (Czechia)
2001-2009
Denaturation of DNA photolyase (deoxyribodipyrimidine photolyase, EC 4.1.99.3) from Escherichia coli with guanidine hydrochloride or acidification to pH 2 released, in addition FAD, a chromophore the spectral and chromatographic properties reduced pterin. Treatment enzyme iodine prior converted stable, oxidized derivative, which was resolved by HPLC into four species identical properties. The same species, distribution, were obtained yeast enzyme. material isolated iodine-oxidized shown be...
Objective The diagnosis of Sjögren's syndrome (SS) in routine practice is largely a clinical one and requires high index suspicion by the treating physician. This great dependence on judgment frequently leads to delayed or misdiagnosis. Tear protein profiles have been proposed as simple reliable biomarkers for SS. Given that cathepsin S activity increased lacrimal glands tears NOD mice (a murine model SS), aim this study was explore utility using tear (CTSS) biomarker Methods A method...
This study reports a general method of labeling enveloped viruses with semiconductor quantum dots (QDs) for use in single virus trafficking studies. Retroviruses, including human immunodeficiency (HIV), could be successfully tagged QDs through the membrane incorporation short acceptor peptide (AP) that is susceptible to site-specific biotinylation and attachment streptavidin-conjugated QDs. It was found this AP tag-based QD had little effect on viral infectivity allowed kinetics...
To explore mechanisms of nanoparticle interactions with and trafficking across lung alveolar epithelium, we utilized primary rat epithelial cell monolayers (RAECMs) an artificial lipid bilayer on filter model (ALBF). Trafficking rates fluorescently labeled polystyrene nanoparticles (PNPs; 20 100 nm, carboxylate (negatively charged) or amidine (positively charged)-modified) in the apical-to-basolateral direction under various experimental conditions were measured. Using confocal laser...
Although inhalation of zinc oxide (ZnO) nanoparticles (NPs) is known to cause systemic disease (i.e., metal fume fever), little about mechanisms underlying injury alveolar epithelium.Investigate ZnO NP-induced epithelium by exposing primary cultured rat epithelial cell monolayers (RAECMs) NPs.RAECMs were exposed apically NPs or, in some experiments, culture fluid containing ZnCl₂ or free Zn released from NPs. Transepithelial electrical resistance (R(T)) and equivalent short-circuit current...
Lacrimal glands (LGs) of male NOD mice, a model Sjögren's syndrome (SjS), exhibit immune cell infiltration and lipid deposition. The mechanism SjS was further investigated by characterizing gene expression profiles mouse LGs in comparison with those healthy control mice. Differentially expressed genes were at the protein level to correlate changes location abundance development disease.
Purpose: To evaluate the efficacy of topical rapamycin in treating autoimmune dacryoadenitis a mouse model Sjögren's syndrome. Methods: We developed poly(ethylene glycol)-distearoyl phosphatidylethanolamine (PEG-DSPE) micelle formulation to maintain solubility. Rapamycin or PEG-DSPE eye drops (vehicle) were administered well-established syndrome disease model, male nonobese diabetic (NOD) mice, twice daily for 12 weeks starting at 8 age. Mouse tear fluid was collected and Cathepsin S,...
Cathepsin S (CTSS) activity is elevated in Sjögren's Syndrome (SS) patient tears. Here we tested whether protease inhibition and cystatin C (Cys C) levels are reduced SS tears, which could lead to enhanced CTSS-driven degradation of tear proteins. CTSS against Cys C, LF sIgA was or healthy control Tears from 156 female subjects (33, SS; 33, rheumatoid arthritis; 31, other autoimmune diseases; 35, non-autoimmune dry eye (DE); 24, controls) were analyzed for LF, levels. showed tears...
Aim: Secretion of proteins into basal tears Parkinson's disease (PD) patients may be altered by changes in nerve function. Materials & methods: Oligomeric α-SynOligo and total α-SynTotal, CCL-2, DJ-1, LF MMP-9 were measured from 93 PD 82 age- sex-equivalent healthy controls. Results: α-SynTotal was decreased (p = 0.0043), whereas < 0.0001) the ratio α-SynOligo/α-SynTotal increased compared with Area under receiver-operating curves contents 0.70 (95% confidence limits: 0.621–0.774) 0.72...
Background Sjögren’s Disease (SjD) is an autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands (LG). The LG produces the protein-rich aqueous component tears, SjD-associated dacryoadenitis (AD) may thus alter tear autoantibody composition. Methods presence tertiary lymphoid structures (TLS) in from two murine models AD, male non-obese diabetic (NOD) insulitis resistant (NOR) mice, were evaluated using immunofluorescence. IgG IgA reactivity serum tears...
The mechanochemical motor proteins of the kinesin and cytoplasmic dynein families play important roles in microtubule-based intracellular motility. Although movement distribution organelles like secretory granules, vesicles, endoplasmic reticulum, chromosomes depend on activity these proteins, little is known about regulation this movement. We report here that hyperphosphorylation components complex by treatment with okadaic acid increases at least 2-fold. stimulation was observed using both...
The acinar epithelial cells of the lacrimal gland exocytose contents mature secretory vesicles containing tear proteins at their apical membranes in response to secretagogues. Here we use time-lapse confocal fluorescence microscopy and recovery after photobleaching investigate changes actin filaments located beneath membrane during exocytosis evoked by muscarinic agonist, carbachol (100 microM). Time-lapse reconstituted rabbit acini transduced with replication-deficient adenovirus GFP-actin...
To investigate the regulation of microtubule-dependent vesicle motility, we have studied effects pharmacological agents on frequency and velocity small movements in intact CV-1 cells. Nocodazole, but not cytochalasin B or D, abolished movements, indicating that these were microtubule actin-dependent. The was stimulated maximally sixfold by okadaic acid from a resting value 1.6 movements/min per microns2 serum-starved Other activators decreasing order effectiveness are fetal calf serum,...
Acute hypertension rapidly decreases proximal tubule (PT) Na + reabsorption, facilitated by a redistribution of PT /H exchangers (NHE3) out the apical brush border, increasing NaCl at macula densa, signal for autoregulation renal blood flow and GFR. This study aimed to determine whether NHE3 activity per transporter during acute time dependence response. Blood pressure was elevated 50–60 mmHg in male Sprague-Dawley rats 5 or 30 min constricting arteries. Renal cortical membranes were...