A5072821705- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Ocular Oncology and Treatments
- Salivary Gland Disorders and Functions
- Connective tissue disorders research
- Intraocular Surgery and Lenses
- Retinal Diseases and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Ophthalmology and Visual Impairment Studies
- Fibroblast Growth Factor Research
- Clusterin in disease pathology
- Proteoglycans and glycosaminoglycans research
- Wnt/β-catenin signaling in development and cancer
- Protease and Inhibitor Mechanisms
- Lysosomal Storage Disorders Research
- Retinal Development and Disorders
- Ocular Infections and Treatments
- Neurological Complications and Syndromes
- Ubiquitin and proteasome pathways
- Calcium signaling and nucleotide metabolism
- Spider Taxonomy and Behavior Studies
- Vascular anomalies and interventions
- Retinoids in leukemia and cellular processes
University of Southern California
2012-2024
Southern California Eye Institute
2014-2024
Doheny Eye Institute
2010-2014
University of Cincinnati
2000-2009
Cleveland Eye Clinic
2008
Cleveland Clinic
2007-2008
Cincinnati Children's Hospital Medical Center
2001-2002
Objective The diagnosis of Sjögren's syndrome (SS) in routine practice is largely a clinical one and requires high index suspicion by the treating physician. This great dependence on judgment frequently leads to delayed or misdiagnosis. Tear protein profiles have been proposed as simple reliable biomarkers for SS. Given that cathepsin S activity increased lacrimal glands tears NOD mice (a murine model SS), aim this study was explore utility using tear (CTSS) biomarker Methods A method...
Lysosomal acid lipase (LAL) is essential for the hydrolysis of triglycerides (TG) and cholesteryl esters (CE) in lysosomes. A mouse model created by gene targeting produces no LAL mRNA, protein, or enzyme activity. The lal−/− mice appear normal at birth, survive into adulthood, are fertile. Massive storage TG CE observed adult liver, adrenal glands, small intestine. age-dependent tissue gross progression this detailed here. Although can be bred to give homozygous litters, they die ages 7 8...
To evaluate the Doheny Eye Institute Experience with Prosthetic Replacement of Ocular Surface Ecosystem (PROSE) scleral lenses for management irregular corneas outcomes based on visual acuity (VA) and function.A retrospective chart review 58 subjects (90 eyes) corneal surfaces referred to PROSE treatment between July 2009 December 2011 was performed. The best-corrected VA before after fitting recorded. A functional assessment also performed using Disease Index, a 12-item questionnaire that...
Cathepsin S (CTSS) activity is elevated in Sjögren's Syndrome (SS) patient tears. Here we tested whether protease inhibition and cystatin C (Cys C) levels are reduced SS tears, which could lead to enhanced CTSS-driven degradation of tear proteins. CTSS against Cys C, LF sIgA was or healthy control Tears from 156 female subjects (33, SS; 33, rheumatoid arthritis; 31, other autoimmune diseases; 35, non-autoimmune dry eye (DE); 24, controls) were analyzed for LF, levels. showed tears...
Investigating stimulation of endogenous wound healing in corneal endothelial cells (CECs) may help address the global shortage donor corneas by decreasing number transplants performed for blindness because dysfunction. We previously reported that IL-1β leads to fibroblast growth factor (FGF2) expression, enhancing migration and proliferation mammalian CECs. However, FGF2 also promotes endothelial-mesenchymal transition, which can lead retrocorneal membrane formation blindness. This prompted...
Glaucoma surgeries, such as trabeculectomy, are performed to lower intraocular pressure reduce risk of vision loss. These surgeries create a new passage in the eye that reroutes aqueous humor outflow subconjunctival space, where fluid is presumably absorbed by conjunctival lymphatics. Here, we characterized development and function ocular lymphatics using transgenic lymphatic reporter mice rats. We found limbal networks progressively formed from primary vessel grows nasal-side medial canthus...
Wnt5a can activate β-catenin-independent pathways for regulation of various cellular functions, such as migration, that play critical roles in wound repair. Investigation signaling may help identify therapeutic targets enhancing corneal endothelial healing could provide an alternative to transplantation patients with blindness from dysfunction. However, cells (CECs) has not been well characterized. In this study, we show transient induction by interleukin-1β (IL-1β) stimulation proceeds...
Protein polymer nanoparticles decorated with the mitogenic human tear protein, lacritin, heal a defect in corneal epithelium faster than soluble control peptide.
Objectives: To investigate the utility of Prosthetic Replacement Ocular Surface Ecosystem (PROSE) scleral lenses in patients with exposure keratopathy, outcomes based on changes visual acuity, function, and corneal staining. Methods: A retrospective interventional case series evaluated treated from September 2009 through June 2014 at a single multi-specialty institutional practice. Eighteen 29 keratoconjunctivitis, lagophthalmos, ectropion, or lid retraction, referred to USC Eye Institute...
Dry eye is a common disorder caused by inadequate hydration of the ocular surface that results in disruption barrier function. The homeostatic protein clusterin (CLU) prominent at fluid-tissue interfaces throughout body. CLU levels are reduced human inflammatory disorders manifest as severe dry eye, well preclinical mouse model for desiccating stress mimics eye. Using this model, we show here prevents and ameliorates remarkable sealing mechanism dependent on attainment critical all-or-none...
Lysosomal acid lipase (LAL) is the essential enzyme for hydrolysis of triglycerides (TGs) and cholesteryl esters (CEs) in lysosomes. Its deficiency produces two human phenotypes: Wolman disease (WD) ester storage (CESD). The LAL null (lal-/-) mouse mimicks aspects WD CESD. potential gene therapy was tested with first-generation adenoviral vectors containing cDNA (Ad-hLAL) by intravenous injection into lal-/- mice. Compared phosphate-buffered saline-injected controls, mice receiving Ad-hLAL...
To develop an in situ model to study biological responses and glaucoma pathology the human trabecular meshwork (TM). Characteristic TM cell- glaucoma-associated markers were localized relation tissue's autofluorescent structural extracellular matrix (ECM) by two-photon excitation fluorescence optical sectioning (TPEF).Human donor corneoscleral (CS) tissue containing intact aqueous drainage tract was incubated with dexamethasone (Dex) or TGF-β1, immunostained for epifluorescence (EF)...
Abstract Background information Interleukin (IL)‐1β is a major pro‐inflammatory cytokine that plays crucial role in the regulation of inflammation and wound healing cornea. Elucidation IL‐1β signalling may help identify therapeutic targets for corneal healing; however, mechanisms such as cell migration, component IL‐1β‐induced response human endothelial cells (CEC), have not been well characterised. Results Stimulation CEC with activated expression fibroblast growth factor 2 (FGF2) resulted...
Purpose: ZEB1 is induced during endothelial-mesenchymal transition (EnMT) in the cornea. Induction of SP1 and SP3 by along with identification putative binding sites promoters EnMT-associated gene lead us to investigate their roles retrocorneal membrane formation corneal endothelium. Methods: Expressions SP1, SP3, EnMT associated genes were analyzed immunoblotting semiquantitative reverse transcription polymerase chain reaction. Accell SMARTpool siRNAs targeting ZEB1, used for knockdown. was...
To compare postoperative complications after Descemet stripping with automated endothelial keratoplasty (DSAEK) in patients and without glaucoma.For this retrospective study a series of 298 DSAEK cases performed at the Doheny Eye Institute were taken, we compared eyes glaucoma on medication (55) or previous surgeries (64) time-matched group all other (179, control).With mean follow-up 1.85 ± 1.12 years, complication rates 12.8%, 11.1%, 26.8% for graft detachment, failure, IOP elevation,...
The cornea is the anterior, transparent tissue of human eye that serves as its main refractive element. Corneal endothelial cells are arranged a monolayer on posterior surface and function pump to counteract leakiness basement membrane. Maintaining in slightly dehydrated state critical for maintenance corneal transparency. Adult G1-arrested, even response injury, leading an age-dependent decline cell density. edema subsequent vision loss ensues when density decreases below threshold. Vision...
In situ 2-photon excitation fluorescence (TPEF) of the human trabecular meshwork (TM) reveals beams heterogeneous autofluorescence (AF) comprising high intensity fluorescent fibers (AF-high) on a background lower (AF-low). To determine sources this AF heterogeneity, we imaged TM to characterize AF, second harmonic generation (SHG) for collagen, and eosin-labeled identifying elastin.
To investigate the relationship between tear concentration of homeostatic protein clusterin (CLU) and dry eye signs symptoms, to characterize CLU protein. Two independent studies were conducted, one in Tucson (44 subjects), other Los Angeles (52 subjects). A cohort study design was employed enroll patients without regard diagnosis. Dry symptoms assessed using clinical tests. Tear samples collected by Schirmer strip, also micropipette at slit lamp when possible. from both sample types...
To measure the wound geometry after femtosecond laser-enabled keratoplasty (FLEK) using optical coherence tomography (OCT).Prospective nonrandomized clinical study and laboratory study.Patients who were candidates for penetrating at an academic referral center.Wound architecture was measured analyzed by OCT in 8 eyes of consecutive patients underwent FLEK. Femtosecond laser lamellar cuts performed on 3 eye bank corneas, diameters OCT.Sutures completely removed average 1 month per decade age...
To report a case of 67-year-old male who was successfully managed over 7-year period for descemetocele secondary to ocular graft versus host disease (oGVHD) using Prosthetic Replacement the Ocular Surface Ecosystem (PROSE) treatment. We previously reported on patient with PROSE device severe dry eyes oGVHD, subsequently developed central corneal descemetocele. The deemed poor surgical candidate due limbal stem cell deficiency oGVHD. Therefore, we elected closely monitor as continued therapy....
Purpose: To evaluate the use of Prosthetic Replacement Ocular Surface Ecosystem (PROSE) scleral lens treatment as an alternative to keratectomy in patients with symptomatic Salzmann's nodular degeneration (SND).Methods: A retrospective chart review from July 2009 May 2013 identified 9 SND who were referred for PROSE evaluation.Patients did not complete fitting or had other corneal comorbidities affecting vision excluded study, and 7 eyes 4 included.Three pseudophakic 1 patient was phakic,...