A5035713285- Systemic Lupus Erythematosus Research
- Liver Disease Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Multiple Myeloma Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Ocular Surface and Contact Lens
- Chronic Myeloid Leukemia Treatments
- Protein Degradation and Inhibitors
- Drug-Induced Hepatotoxicity and Protection
- Hepatitis C virus research
- Salivary Gland Disorders and Functions
- Immune cells in cancer
- Liver Diseases and Immunity
- Cancer Research and Treatments
- Rheumatoid Arthritis Research and Therapies
- Cancer Immunotherapy and Biomarkers
- Diabetes and associated disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Mast cells and histamine
The University of Texas Southwestern Medical Center
2015-2025
Epworth Hospital
2021-2024
Walter and Eliza Hall Institute of Medical Research
2021-2024
Southwestern Medical Center
2012-2024
Russian Medical Academy of Continuous Professional Education
2023
Monash Health
2018
Recent achievement in genetics and epigenetics has led to the exploration of pathogenesis systemic lupus erythematosus (SLE). Identification differentially expressed genes their regulatory mechanism(s) at whole-genome level will provide a comprehensive understanding development SLE its devastating complications, nephritis (LN). We performed transcription DNA methylation analysis PBMC 30 patients, including 15 with LN (SLE LN+) without LN−), 25 normal controls (NC) using HumanHT-12 Beadchips...
Prediction of hepatocellular carcinoma (HCC) risk is an urgent unmet need in patients with nonalcoholic fatty liver disease (NAFLD). In cohorts 409 NAFLD from multiple global regions, we defined and validated hepatic transcriptome serum secretome signatures predictive long-term HCC NAFLD. A 133-gene signature, prognostic signature (PLS)–NAFLD, predicted incident over up to 15 years longitudinal observation. High-risk PLS-NAFLD was associated IDO1 + dendritic cells dysfunctional CD8 T...
An accurate diagnosis of septic versus reactive or autoimmune arthritis remains clinically challenging. A multi-omics strategy comprising metagenomic and proteomic technologies were undertaken for children diagnosed with presumed to advance clinical diagnoses care affected individuals. Twelve suspected prospectively enrolled compare standard tests a rapid approach. The combined bacterial 16S rRNA metagenomics, single cell transcriptomics, proteomics on knee joint fluid specimens. diagnostic...
Background Sjögren’s Disease (SjD) is an autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands (LG). The LG produces the protein-rich aqueous component tears, SjD-associated dacryoadenitis (AD) may thus alter tear autoantibody composition. Methods presence tertiary lymphoid structures (TLS) in from two murine models AD, male non-obese diabetic (NOD) insulitis resistant (NOR) mice, were evaluated using immunofluorescence. IgG IgA reactivity serum tears...
Objective Disordered T peripheral helper (Tph)‐B cell interactions have been implicated in several forms of inflammatory arthritis, including oligoarticular juvenile idiopathic arthritis (oligo JIA). We sought to evaluate the Tph‐B axis oligo JIA through an analysis intra‐articular B cells. Methods cells from blood and synovial fluid (SF) 44 children with were compared those tonsils controls. Flow cytometry, receptor (BCR) repertoire analysis, autoantibody profiling used characterize Results...
Objective . The study goals were to evaluate performance of SLE classification criteria, define patients with incomplete lupus erythematosus (ILE), and probe for features in these that might be useful as indicators disease status hydroxychloroquine response. Methods Patients ILE (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>N</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">70</mml:mn></mml:math>) id="M2"><mml:mi>N</mml:mi><mml:mo>=</mml:mo><mml:mn...
Drug-induced liver injury (DILI) sometimes presents with an autoimmune hepatitis-like phenotype (AI-DILI), and it is challenging to distinguish from de novo hepatitis (AIH). We conducted a study identify autoantibodies unique AI-DILI by profiling serum autoantibodies. Autoantibodies were quantified using autoantigen array containing 94 autoantigens four groups: (n = 65), DILI controls 67), AIH 17), healthy (HCs; n 30). In 37 patients AI-DILI, samples also collected 6 months after...
Abstract Introduction Oxidative stress is implicated in tissue inflammation, and plays an important role the pathogenesis of immune-mediated nephritis. Using anti-glomerular basement membrane antibody-induced glomerulonephritis (anti-GBM-GN) mouse model, we found that increased expression glutathione S-transferase Mu 2 (GSTM2) was related to reduced renal damage caused by anti-GBM antibodies. Furthermore, mesenchymal stem cell (MSC)-based therapy has shed light on treatment kidney diseases....
B cell activation is regulated by the stimulatory or inhibitory co-receptors of receptors (BCRs). Here, we investigated signaling mechanism Fc receptor-like 1 (FcRL1), a newly identified BCR co-receptor. FcRL1 was passively recruited into immunological synapses upon engagement in absence cross-linking, suggesting that may intrinsically regulate and function. cross-linking alone led to phosphorylation intracellular Y
Background: Accurate non-invasive prediction of long-term hepatocellular carcinoma (HCC) risk in advanced liver fibrosis is urgently needed for cost-effective HCC screening; however, this currently remains an unmet need.Methods: A serum-protein-based prognostic secretome signature (PLSec) was bioinformatically derived from previously validated hepatic transcriptome signatures and optimized 79 patients with fibrosis. We independently PLSec 331 cirrhosis mixed etiologies (validation set 1...
Previously we have shown that kallikreins (klks) play a renoprotective role in nephrotoxic serum induced nephritis. In this study, used mesenchymal stem cells (MSCs) as vehicles to deliver klks into the injured kidneys and measured their therapeutic effect on experimental antibody nephritis lupus Human KLK-1 (hKLK1) gene was transduced murine MSCs using retroviral vector generate stable cell line, hKLK1-MSC, expressing high levels of hKLK1. 129/svj mice subjected anti-GBM were transplanted...
To elucidate the role of oxidation resistance 1 (OXR1) gene. Oxidative stress plays a pivotal in pathogenesis immune-mediated nephritis. Recently we identified is conventionally expressed eukaryotes and has an ability to prevent oxidative damage caused by various stresses. However protective effect OXR1 immune-associated inflammatory response not clear will be investigated this study.We utilized mesenchymal stem cells (MSCs) as vehicles carry into injured kidneys nephritis model mice...
Introduction: The mechanisms underlying chronic obstructive pulmonary disease (COPD) remain unclear. Genetic and genomic changes may play a significant role in the pathogenesis of COPD. Identification differentially expressed genes miRNAs their regulatory at whole-genome level will provide comprehensive understanding development Methods: Peripheral blood mononuclear cells (PBMCs) from 12 patients with COPD normal controls were examined miRNA mRNA expression levels using Affymetrix GeneChip....
Abstract Objective To ascertain whether engineered expression of kallikreins within the kidneys, using an inducible Cre/loxP system, can ameliorate murine lupus nephritis. Methods In mice with a lupus‐prone genetic background, we tamoxifen‐inducible Cre recombinase under control kidney‐specific promoter whose activation initiates kallikrein‐1 kidneys. These transgenic were injected either tamoxifen or vehicle at age 2 months and then monitored for 8 kallikrein disease. Results Elevated was...
The interaction between antigens and antibodies (B cell receptors, BCRs) is the key step underlying function of humoral immune system in various biological contexts. capability to profile landscape antigen-binding affinity a vast number BCRs will provide powerful tool reveal novel insights at unprecedented levels yield tools for translational development. However, current experimental approaches profiling antibody-antigen interactions are costly time-consuming, can only achieve low-to-mid...
Abstract This study tested the hypothesis that B cells from salivary tissue are distinct in terms of proliferative capacity, immunoglobulin M secretion, repertoire, and autoantibody enrichment Sjögren’s syndrome. We sorted purified spleen, cervical lymph nodes, submandibular glands a primary syndrome mouse model (Id3−/−). Enzyme-linked immunospot proliferation assays were performed with stimulated cells. single-cell gland mice sequenced heavy-chain variable regions. Finally, autoantigen...