A5055261184- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- 3D Printing in Biomedical Research
- Systemic Lupus Erythematosus Research
- Psoriasis: Treatment and Pathogenesis
- Tryptophan and brain disorders
- Gut microbiota and health
- Platelet Disorders and Treatments
- NF-κB Signaling Pathways
- Stress Responses and Cortisol
- Cancer Research and Treatments
- Microfluidic and Bio-sensing Technologies
- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Bone Metabolism and Diseases
- Hematopoietic Stem Cell Transplantation
- Glutathione Transferases and Polymorphisms
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
Cleveland Clinic Lerner College of Medicine
2023
Cleveland Clinic
2022
The University of Texas at Austin
2020-2022
The University of Texas Southwestern Medical Center
2010-2016
Seoul National University Bundang Hospital
2014
Southwestern Medical Center
2013
Seoul National University
2010-2013
Catholic University of Korea
2002-2012
Seoul St. Mary's Hospital
2002-2008
Dongguk University
2007
Abstract IL-23 is a heterodimeric cytokine composed of p19 subunit and the p40 IL-12. has proinflammatory activity, inducing IL-17 secretion from activated CD4+ T cells stimulating proliferation memory cells. We investigated pathogenic role in mice lacking IL-1R antagonist (IL-1Ra−/−), an animal model spontaneous arthritis. was strongly expressed inflamed joints IL-1Ra−/− mice. Recombinant adenovirus expressing mouse (rAd/mIL-23) significantly accelerated this joint inflammation destruction....
Here, we report a pathomimetic Leaky Gut Chip that recapitulates increased epithelial permeability and intestinal inflammation to assess probiotic intervention as live biotherapeutics. We leveraged mechanodynamic human gut-on-a-chip (Gut Chip) recreates three-dimensional layers in controlled oxygen gradient biomechanical cues, where the addition of cocktail pro-inflammatory cytokines, TNF-α IL-1β, reproducibly induced impaired barrier followed by inflammation. This inflamed leaky epithelium...
Abstract Objective Poly(lactic‐co‐glycolic acid) (PLGA), a biodegradable polymer, is carrier for drug delivery systems. This study was undertaken to investigate the tolerogenic effect of single administration PLGA entrapping type II collagen (CII) on development collagen‐induced arthritis (CIA). Methods The biophysical properties nanoparticles CII (PLGA‐CII) were investigated by in vitro release testing CII, immunohistochemistry analysis, and electron microscopy. PLGA‐CII fed singly animals...
Bone marrow-derived mesenchymal stem cells (MSCs) can prevent various autoimmune diseases. We examined the therapeutic potential of transforming growth factor β (TGFβ)-transduced MSCs in experimental arthritis, using an accepted animal model collagen-induced arthritis (CIA).DBA/1J mice with CIA were treated syngeneic TGFβ-induced MSCs, whereas control received either vehicle or alone. Arthritis severity was assessed by clinical and histologic scoring. TGFβ-transduced tested for their...
Abstract IL-23, a clinically novel cytokine, targets CD4+ T cells. Recent IL-1Ra−/− mouse studies have demonstrated that IL-23 indirectly stimulates the differentiation of osteoclast precursors by enhancing IL-17 release from IL-17, in turn, osteoclastogenesis precursor In this study, we found up-regulates receptor activator NF-κB ligand expression cells, and thus contributes to osteoclastogenesis. This indirect pathway is mediated STAT3. We also can influence positively under special...
The activity of one the major catechins in Green Tea, polyphenol (−)-epigallocatechin-3-gallate (EGCG), has been shown to have a variety health benefits. Recent studies suggest that EGCG can modulate both innate and adaptive arms immune system. goal current was examine immunomodulatory effects mechanisms action on experimental arthritis mice. (10 mg/kg) administered by oral gavage after CIA induction, while control mice were phosphate buffered saline (PBS). Disease studied groups Phenotypes...
The regeneration of the mucosal interface human intestine is critical in host–gut microbiome crosstalk associated with gastrointestinal diseases. biopsy-derived intestinal organoids provide genetic information patients physiological cytodifferentiation. However, enclosed lumen and static culture condition substantially limit utility patient-derived for microbiome-associated disease modeling. Here, we report a patient-specific three-dimensional (3D) physiodynamic interface-on-a-chip (PMI...
Recent advances in canine intestinal organoids have expanded the option for building a better vitro model to investigate translational science of physiology and pathology between humans animals. However, three-dimensional geometry enclosed lumen considerably hinder access apical side epithelium investigating nutrient drug absorption, host-microbiome crosstalk, pharmaceutical toxicity testing. Thus, creation polarized epithelial interface accessible from or basolateral is critical. Here, we...
In a conventional culture of three-dimensional human intestinal organoids, extracellular matrix hydrogel has been used to provide physical space for the growth and morphogenesis organoids in presence exogenous morphogens such as Wnt3a. We found that embedded dome-shaped show significant size heterogeneity different locations inside hydrogel. Computational simulations revealed instability diffusion limitation Wnt3a constitutively generate concentration gradient The location-dependent dome...
Abstract We tested the impact of CD40 engagement on production vascular endothelial growth factor (VEGF) from rheumatoid synovial fibroblasts. Fibroblast-like cells (FLS) were prepared tissues arthritis patients and cultured in presence ligand-transfected (CD40L+) L cells. VEGF levels determined culture supernatants by ELISA. Stimulation FLS CD40L+ increased 4.1-fold over constitutive unstimulated FLS. The CD40L activated T fluid also up-regulated Neither indomethacin nor Abs to IL-1β,...
Matrix metalloproteinase-9 (MMP-9) has been implicated in the pathogenesis of cancer, autoimmune disease, and various pathologic conditions characterized by excessive fibrosis. In this study, we investigated expression MMP-9 its clinical significance systemic sclerosis (SSc). The patients (n = 42) with SSc had higher concentrations tissue inhibitor metalloproteinase-1 (TIMP-1) a ratio to TIMP-1 sera than healthy controls 32). Serum were significantly diffuse type 23) limited 19). correlated...
Tremendous advances have been made toward accurate recapitulation of the human intestinal system in vitro to understand its developmental process, and disease progression. However, current models are often confined 2D or 2.5D microarchitectures, which is difficult mimic systemic level complexity native tissue. To overcome this problem, physiologically relevant developed with a 3D hollow tubular structure using bioprinting strategy. A tissue-specific biomaterial, colon-derived decellularized...
Abstract Objective To investigate the effector function exerted by type II collagen (CII)–stimulated T cells on rheumatoid arthritis (RA) fibroblast‐like synoviocytes (FLS), and to determine their contribution RA pathogenesis. Methods We used enzyme‐linked immunosorbent assays measure levels of interleukin‐15 (IL‐15), tumor necrosis factor α (TNFα), IL‐18 production FLS that were cocultured with antigen‐activated cells. Likewise, we analyzed interferon‐γ (IFNγ) IL‐17 coincubated FLS....
Abstract Objective Although oral tolerance is a well‐known phenomenon, the role of dendritic cells (DCs) not well characterized. This study was conducted to better understand differential played by each Peyer's patch DC subset in induction type II collagen (CII) murine collagen‐induced arthritis (CIA). Methods CII fed 6 times DBA/1 mice beginning 2 weeks before immunization, and effect on assessed. We compared proportion CD11c+,CD11b+ DCs CD11c+,CD8α+ patches CII‐fed tolerized phosphate...
Abstract Background It is well known that interferon (IFN)-α important to the pathogenesis of systemic lupus erythematosus (SLE). However, several reports have indicated number IFN-α producing cells are decreased or their function defective in patients with SLE. We studied plasmacytoid dendritic (pDCs) under persistent stimulation Toll-like receptor (TLR)9 via a TLR9 ligand (CpG ODN2216) SLE serum. Methods The concentrations were determined serum and culture supernatant peripheral blood...
Abstract Objective To determine the antiangiogenic effect of cyclosporin A (CSA) in rheumatoid arthritis (RA). Methods We investigated CSA on production vascular endothelial growth factor (VEGF) by synovial fibroblasts. Fibroblast‐like synoviocytes (FLS) were prepared from tissues RA patients, and cultured presence CSA. The VEGF FLS was measured culture supernatants enzyme‐linked immunosorbent assay. messenger RNA (mRNA) expression activator protein 1 (AP‐1) binding activity for...
Induction of oral tolerance has long been considered a promising approach to the treatment chronic autoimmune diseases, including rheumatoid arthritis (RA). Oral administration type II collagen (CII) proven improve signs and symptoms in RA patients without troublesome toxicity. To investigate mechanism immune suppression mediated by orally administered antigen, we examined changes serum IgG subtypes T-cell proliferative responses CII, generation IL-10-producing CD4+CD25+ subsets an animal...