A5001664560- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Essential Oils and Antimicrobial Activity
- Epigenetics and DNA Methylation
- Ginseng Biological Effects and Applications
- Invertebrate Immune Response Mechanisms
- Phytochemistry and biological activity of medicinal plants
- Pharmacological Effects of Natural Compounds
- RNA modifications and cancer
- Natural product bioactivities and synthesis
- Analytical Chemistry and Chromatography
- Pharmacological Effects of Medicinal Plants
- Galectins and Cancer Biology
- Inflammatory mediators and NSAID effects
- Land Use and Ecosystem Services
- Insect Pest Control Strategies
- Plant Pathogens and Fungal Diseases
- Analytical Methods in Pharmaceuticals
- Powdery Mildew Fungal Diseases
- Single-cell and spatial transcriptomics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Vascular Malformations Diagnosis and Treatment
- Studies on Chitinases and Chitosanases
Broad Institute
2018-2024
Phenikaa University
2020-2024
Phenikaa (Vietnam)
2021
New York University
2019
Brown University
2015-2018
Vietnam National University, Hanoi
2017
Significance Gene expression is controlled by sequence-specific transcription factors (TFs), which bind to regulatory sequences in DNA. The degree the arrangement of motif sites within elements determines their function remains unclear. Here, we show that positional distribution TF nucleosome-depleted regions DNA fall into six distinct classes. These patterns are highly consistent across cell types and bring together have similar functional binding properties. Furthermore, position appears...
Hermansky-Pudlak syndrome (HPS) comprises a group of inherited disorders caused by mutations that alter the function lysosome-related organelles. Pulmonary fibrosis is major cause morbidity and mortality in patients with subtypes HPS-1 HPS-4, which both result from defects biogenesis organelle complex 3 (BLOC-3). The prototypic chitinase-like protein chitinase 3–like–1 (CHI3L1) plays protective role lung ameliorating cell death stimulating fibroproliferative repair. Here, we demonstrated...
Abstract Hermansky–Pudlak syndrome (HPS) comprises a group of inherited disorders caused by mutations that alter the function lysosome-related organelles. Pulmonary fibrosis is major cause morbidity and mortality in HPS-1 HPS-4 patients. However, mechanisms underlie exaggerated injury fibroproliferative repair responses HPS have not been adequately defined. In particular, although Galectin-3 (Gal-3) dysregulated HPS, its roles pathogenesis addition, chitinase 3-like 1 (CHI3L1) receptors play...
The transcriptional repression of alternative lineage genes is critical for cell fate commitment. Mechanisms by which locus-specific gene silencing initiated and heritably maintained during division are not clearly understood. To study the maintenance silent states, we investigated how Cd4 stably repressed in CD8 + T cells. Through CRISPR shRNA screening, identified histone chaperone CAF-1 as a component repression. We found that large subunit CAF-1, Chaf1a, requires N-terminal KER domain to...
Abstract Mammalian genomes harbor millions of noncoding elements called enhancers that quantitatively regulate gene expression, but it remains unclear which genes. Here we describe an experimental approach, based on CRISPR interference, RNA FISH, and flow cytometry (CRISPRi-FlowFISH), to perturb in the genome, apply test >3,000 potential regulatory enhancer-gene connections across multiple genomic loci. A simple equation a mechanistic model for enhancer function performed remarkably well...
Abstract Genome-wide association studies (GWAS) have discovered thousands of risk loci for common, complex diseases, each which could point to genes and gene programs that influence disease. For some it has been observed GWAS signals converge on a smaller number biological programs, this convergence can help identify causal 1–6 . However, identifying such remains challenging: locus many candidate genes, might act in one or more possible unclear disease risk. Here, we developed new approach...
Abstract Genome-wide association studies have now identified tens of thousands noncoding loci associated with human diseases and complex traits, each which could reveal insights into biological mechanisms disease. Many the underlying causal variants are thought to affect enhancers, but we lacked genome-wide maps enhancer-gene regulation interpret such variants. We previously developed Activity-by-Contact (ABC) Model predict connections demonstrated that it can accurately results CRISPR...
Vietnamese ginseng (VG) (Panax vietnamensis, Araliaceae) is indigenous in the central highlands of Vietnam and southernmost distribution Panax genus. Compared to long history use overall research on Korean American ginseng, up‑to‑date publication VG relatively much less extensive. The studies have been reported focusing phylogenetic analysis, phytochemistry, pharmacological activity. To date, there no systematic review VG. In this review, phytochemical profile including 52 individual...
Plant pathogenic fungi and bacteria cause large losses in the yield quality of important crops. During screening bioactive plants against plant pathogens, we found that methanol extract aerial part Rumex chinensis exhibited potent efficacy various diseases. The was effectively active phytopathogenic Blumeria graminis f. sp. hordei Colletotrichum coccodes diseases vivo. n-Hexane ethyl acetate layers obtained by liquid-liquid partitioning strongly suppressed these fungal with control values...
century. But with the advent of antibiotics starting around 80 years ago, TB then became a curable disease. It had no longer deadly effectiveness as it did for ancient Roman and Victorian populations. As an effect, Mycobacterium tuberculosis, causative agent discovered by Robert Koch in 1882, finally faded out limelight.
Abstract Building upon prior research demonstrating the excellent antifungal and nematicidal efficacy of Annona squamosa Lin. seed extracts, this study extends its scope to investigate similar properties in extracts from two other Annonaceae species: muricata glabra The focus is on assessing their effectiveness against tomato late blight (TLB) wheat leaf rust (WLR) diseases, as well broader activity Colletotrichum sp. Fusarium A. extract exerted a superior control TLB (85 % at 62.5 μg/mL)...
The landscapes in the Hinh River Basin are crucial and highly sensitive to climate change for coastal province of Phu Yen entire south-central region Vietnam, offering vital environmental services its downstream areas. has a rich system rivers streams abundant surface water resources. However, it remains one region's top localities at risk very vulnerable region. This study aims evaluate changes landscape (LC) over 10 years (2010-2023) predict LC next six using machine-learning (ML)...
Abstract Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters. One model for specificity of enhancer-promoter different promoters might have sequence-encoded preferences distinct classes enhancers, example mediated interacting sets transcription factors or cofactors. This “biochemical compatibility” has been supported observations at individual and genome-wide measurements Drosophila . However, degree to which are intrinsically...
In this study, a high-performance liquid chromatographic method with fluorecence detection was developed for the quantification of flurbiprofen in tablets. A C8 reverse phase column (5 μm, 120 Å, 4.6×150 mm) used mobile acetonitrile : water glacial acetic acid (65 32.5 2.5, v/v/v) . The flow rate 1 ml/min and effluent monitored within 8 minutes. excitation emission wavelength were respectively 247 nm 312 nm. temperature cell set at 45˚C detector sensitivity 5. retention time found to be 3.78...
Panax bipinnatifidus Seem. belonging to the family Araliaceae has been used as a folk medicine in Viet Nam. Our ongoing study on bioactive compounds from collected Sapa, Lao Cai resulted isolation of two saponins together with well-known saponin Stipuleanoside R2. Their structures were identified 3-O-β-D-glucopyranosyl-(1→3)-β-D-glucuronopyranosyloleanolic acid (1) and R1 (2) based NMR spectroscopic data well comparison literature data. For first time, was found genus Panax. On biological...
Từ dịch chiết ethanol của lá cây diễn (Dicliptera chinensis (L.) Nees) thu hái ở tỉnh Nam Định, sử dụng phương pháp sắc ký đã phân lập được hai hợp chất terpenoid. Các này xác định là: 3β-hydroxylup-20 (29)-20(29)-en (1), stigmast-5,22-dien-3-β-ol (2). Cấu trúc các dựa trên dữ liệu phổ khối lượng và cộng hưởng từ hạt nhân kết so sánh với công bố trong tài tham khảo. Đây là 2 lần đầu tiên