A5084864470- Leptospirosis research and findings
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Veterinary medicine and infectious diseases
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Herpesvirus Infections and Treatments
- Cell Adhesion Molecules Research
- Viral gastroenteritis research and epidemiology
- Autoimmune and Inflammatory Disorders Research
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Connective Tissue Growth Factor Research
- Exercise and Physiological Responses
- Viral Infections and Outbreaks Research
- Biosensors and Analytical Detection
- Muscle metabolism and nutrition
- Sepsis Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Antimicrobial Peptides and Activities
- Virus-based gene therapy research
Chulalongkorn University
2013-2023
Thai Red Cross Society
2012-2015
Mahidol Oxford Tropical Medicine Research Unit
2015
Mahidol University
2015
Creative Research Enterprises (United States)
2015
Bangkok University
2014
Hospital for Tropical Diseases
2013
King Chulalongkorn Memorial Hospital
2012
University of Kansas
1995-2002
When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary infection is uncommon. However, as epithelium injured and barrier function lost, products entering the circulation might contribute to pathophysiology of COVID-19. We studied 19 adults, severely ill patients infection, who were admitted King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March 17th April 2020. Blood samples on days 1, 3, 7 enrollment analyzed for endotoxin activity...
Abstract This study monitored the long-term immune response to severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infection in patients who had recovered from disease (COVID)-19. Anti-nucleocapsid immunoglobulin G (anti-N IgG) titer serum samples collected at a single (N = 302) or multiple time points 229) 3–12 months after COVID-19 symptom onset SARS-CoV-2 detection specimens was measured by semiquantitative chemiluminescent microparticle immunoassay. The 531 (966 specimens) were...
Between 2018 and 2019, the incidence of chikungunya was approximately 15,000 cases across 60 provinces in Thailand. Here, clinical presentations chikungunya, emergent pattern, genomic diversity virus (CHIKV) causing this massive outbreak were demonstrated. A total 1,806 sera samples from suspected collected 13 Thailand, tested for presence CHIKV RNA, IgG, IgM using real-time PCR, enzyme-linked immunoassay (ELISA), commercial (rapid test). The phylogenetic tree whole-genome E1 constructed...
Understanding antibody responses after natural severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can guide the disease 2019 (COVID-19) vaccine schedule, especially in resource-limited settings. This study aimed to assess dynamics of SARS-CoV-2 antibodies, including anti-spike protein 1 (S1) immunoglobulin (Ig)G, anti-receptor-binding domain (RBD) total Ig, anti-S1 IgA, and neutralizing against wild-type over time a cohort patients who were previously infected with...
Abstract Regulation of T cell activation requires two signals. First, appropriately presented Ag in the context MHC interacts with receptor-CD3 complex. The best-studied second signal is CD28, which resides on and responds to its counter receptor, B7. A also can be delivered through LFA-1 residing cell, responding receptor ICAM-1 a different cell. Characterization tied ability costimulate (along stimulation TCR) proliferation, IL-2 secretion, coactivation phosphatidylinositol 3-kinase. We...
Determination of antibody titer by microscopic agglutination test (MAT) has been used as a tool for leptospirosis diagnosis. Four fold or greater rise in titers between acute and convalescent sera suggests recent Leptospira infection. In addition, results obtained MAT have to predict infecting serovars. However, cross reactivity among various serovars reported when patient were tested with battery This study demonstrates cross-reactivity several was performed on sera. The data support role...
To investigate cytokine profile in patients with chikungunya virus (CHIKV) infection.Twenty eight pairs of serum samples collected from CHIKV infected during the outbreak fever South Thailand 2008 were obtained. A multiple assay for detection 17 cytokines was performed.In acute stage infection, had significantly higher levels interleukin-6, granulocyte colony-stimulating factor, granulocyte-macrophage monocyte chemotactic protein 1 and tumor necrosis factor alpha than control (P<0.001,...
AKI is one of the most serious complications leptospirosis, an important zoonosis in tropics. Recently, NGAL, novel biomarkers, extensively studied various specific settings such as sepsis, cardiac surgery, and radiocontrast nephropathy. In this multicenter study, we aimed to study role NGAL early marker outcome predictor leptospirosis associated AKI. Patients who presented with clinical suspiciousness were prospectively enrolled 9 centers from August 2012 November 2014. The first day...
Abstract The coronavirus disease 2019 pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) is a major global concern. Several SARS-CoV-2 gene mutations have been reported. In the current study associations between variation and exposure history during first wave of outbreak in Thailand January May 2020 were investigated. Forty samples collected at different time points outbreak, parts genome sequence used to assess genomic patterns. phylogenetics 40 clustered into L, GH, GR, O...
Chikungunya virus infection has recently emerged in several countries. The inflammatory response is suggested to be involved the pathology observed this infectious disease. Interleukin-18 (IL-18) an inducer of interferon-γ (IFN-γ) production and been shown play a role diseases. IL-18 binding protein (IL-18BP) natural regulator IL-18. In study, we determined levels IL-18BP patients with infection. Acute convalescent sera were collected from each patient. both measured by ELISA assays. higher...
Vitamin D deficiency was common among patients with chronic hepatitis C (CHC) and had negative influence on treatment outcome. Correction of vitamin improved response. Interferon gamma-induced protein 10 (IP-10) enzyme dipeptidyl peptidase-4 (DPP IV) involved in inflammatory responses CHC. Their higher levels at pretreatment CHC could predict poorer responses. suppressed expression IP-10 from monocytes vitro. In patients, DPP IV regulation. We hypothesized that correction insufficiency or...
Hepatic fibrosis is the net accumulation of matrix tissue components which controlled by pro-fibrolytic enzymes, metalloproteinases (MMPs), and pro-fibrotic cytokine, TGF-β1, inhibitors MMPs (TIMPs). Vitamin D (VD) supplementation has been shown to reverse these processes in vitro vivo. This study sought determine effect VD on serum fibrotic markers chronic hepatitis C (CHC) patients. Fifty-four CHC patients with deficiency were randomized into two groups, a group (n = 29) placebo 29). The...
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although Thailand has been fairly effective at controlling spread of COVID-19, continued surveillance and information on antibody response in recovered patients their close contacts remain necessary absence approved vaccines antivirals. Here, we examined 217 COVID-19 to assess viral RNA shedding residual antibodies against SARS-CoV-2. We also evaluated blood samples from 308...
Abstract Background Efficient monitoring and control of coronavirus disease 2019 (COVID-19) require access to diagnostic tests, serological testing is desirable. In the current study, antibodies were investigated in patients recently diagnosed with severe acute respiratory syndrome 2 (SARS-CoV-2) infection. Methods Cross-sectional data obtained from 245 whom SARS-CoV-2 infection had been confirmed via real-time reverse transcriptase-polymerase chain reaction between March October 2020. Serum...
Abstract Intercellular adhesion molecules (ICAM)-1 and -3 coexist on T lymphocytes are counter-receptors for the integrin LFA-1. Signaling through ICAM-3 stimulates a number of cell functions involves phosphorylation Fyn, Lck, CD45, other proteins. In contrast, this type specific signaling event has not been described ICAM-1. Here, tyrosine cellular proteins was examined after cross-linking Tyrosine 34-kDa cdc2 protein kinase induced transiently stimulation leukemic line, Molt-3, or...
Eighty-seven high-risk individuals in Thailand who had received a complete course of recombinant HBV vaccine 18–20 y ago were investigated with regard to their immunological memory. To evaluate humoral immunity, anti-HBs antibody titers measured. Cellular immunity was determined by ELISPOT detect HBV-specific IFN-γ–producing cells. Overall 83.9% participants developed circulating (titer ≥1 mIU/mL) and 58.6% seroprotected ≥10 mIU/mL). As for cellular 50.6% positive on ELISPOT. Moreover, there...
BACKGROUND Hepatitis E virus (HEV) infection in several industrialized and developing countries is associated with the consumption of pork other meat products, an exposure risk among majority blood donors. We aimed to evaluate prevalence HEV plasma from healthy donors Thailand. STUDY DESIGN AND METHODS screened samples collected between October December 2015, 30,115 individual 5020 pools six, for RNA using in‐house real‐time reverse‐transcription polymerase chain reaction (RT‐PCR)....
Abstract Purpose When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary infection is uncommon. However, as epithelium are injured and barrier function lost, products entering the circulation might contribute to pathophysiology of COVID-19. Methods We studied 19 adults, severely ill patients infection, who were admitted King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13 th March 17 April 2020. Blood samples on day 1, 3, 7 enrollment analyzed...