A5006217863- interferon and immune responses
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Mosquito-borne diseases and control
- RNA and protein synthesis mechanisms
- Innovative Microfluidic and Catalytic Techniques Innovation
- MicroRNA in disease regulation
- Oral microbiology and periodontitis research
- RNA modifications and cancer
- Viral Infections and Vectors
- Leptospirosis research and findings
- Respiratory viral infections research
- Virus-based gene therapy research
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Salivary Gland Disorders and Functions
Chulalongkorn University
2020-2023
Due to the common symptoms of COVID-19, patients are similar influenza-like illness. Therefore, detection method would be crucial discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based was applied for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), A virus, B virus which a practical attractive application screening with COVID-19 in areas limited resources. The limit SARS-CoV-2, A, 10, 10 3 , copies/reaction, respectively....
Background One of the key barriers preventing rapid diagnosis leptospirosis is lack available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined with isothermal amplification detect leptospires from extracted patient DNA samples. Methodology/Principal findings A Recombinase Polymerase Amplification (RPA)-CRISPR/Cas12a-fluorescence assay...
Rapid, accurate, and low-cost detection of SARS-CoV-2 is crucial to contain the transmission COVID-19. Here, we present a cost-effective smartphone-based device coupled with machine learning-driven software that evaluates fluorescence signals CRISPR diagnostic SARS-CoV-2. The consists three-dimensional (3D)-printed housing optic components allow excitation fluorescent reporters selective emission smartphone. Custom equipped binary classification model has been developed quantify acquired...
The coronavirus (COVID-19) global pandemic has impacted the health of almost everyone, including changes in their salivary microbiota. Since 2019, there been an increase number new COVID-19 cases Thailand. Therefore, active case finding is important for early detection and epidemic control. Moreover, dynamic bacteriome asymptomatic are largely unknown. This research aimed to investigate compare co-infectious bacterial pathogens positive group negative group, based on novel nanopore...
The influenza virus is a cause of seasonal epidemic disease and enormous economic injury. best way to control outbreaks through vaccination. Madin-Darby canine kidney cell line (MDCK) currently approved manufacture vaccines. However, the viral load from cell-based production limited by host interferons (IFN). Interferon regulating factor 7 (IRF7) transcription for type-I IFN that plays an important role in anti-viral mechanism eliminating viruses. We developed IRF7 knock-out MDCK cells...
In this long-term storage study, we optimized the lyophilization conditions of each reaction stage a nucleic acid-based assay for SARS-CoV-2 detection. The stability testing demonstrated that dried reactions from all 3 steps (cDNA synthesis, isothermal amplification and detection) can be kept at -20°C or 4°C up to 6 months, respectively, whereas, if stored 25°C 37°C, reagents only could few days without quality loss. This suggests have until needed. Moreover, is now simpler perform as...
The influenza A viruses have high mutation rates and cause a serious health problem worldwide. Therefore, this study focused on genome characterization of the isolated from Thai patients based next-generation sequencing technology. nasal swabs were collected with influenza-like illness in Thailand during 2017-2018. Then, detected by reverse transcription-quantitative polymerase chain reaction MDCK cells. viral genomes amplified sequenced Illumina MiSeq platform. Whole sequences used for...
Influenza B virus is a member of the Orthomyxoviridae family which can infect humans and causes influenza. Although it not pandemic like influenza A virus, nevertheless affects millions people worldwide annually. MicroRNAs are small non-coding RNAs regulating gene expression at posttranscriptional level. They play various important roles in cellular processes including response to viral infection. MiRNA profiles from our previous study suggested that miR-30e-3p was one upregulated miRNAs...
COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus affecting world population. Early detection has become one of most successful strategies to alleviate epidemic and pandemic this contagious coronavirus. Surveillance testing programs have been initiated in many countries worldwide prevent outbreak COVID-19. In study, we demonstrated that our previously established clustered regularly interspaced short palindromic repeats...
Surveillance testing is a key strategy to control the spread of COVID-19. Unlike gold standard method, quantitative reverse transcription polymerase chain reaction (RT-qPCR), CRISPR diagnostics have recently become more appealing alternative as they are proven be faster, simpler, and affordable. However, current diagnostic readouts typically non-quantitative, making them error-prone lacking crucial information viral load. To further improve we developed custom computer vision algorithm that...