A5009283809- Gut microbiota and health
- Diet and metabolism studies
- Tryptophan and brain disorders
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Reproductive tract infections research
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- Microbial Metabolism and Applications
- Microbial Community Ecology and Physiology
- Dialysis and Renal Disease Management
- Adolescent Sexual and Reproductive Health
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Biosensors and Analytical Detection
- Parasite Biology and Host Interactions
- Maternal Mental Health During Pregnancy and Postpartum
- Parasites and Host Interactions
- Urinary Tract Infections Management
- Advanced Chemical Sensor Technologies
- Tannin, Tannase and Anticancer Activities
- Bacterial Identification and Susceptibility Testing
- Sexual function and dysfunction studies
- Cancer Genomics and Diagnostics
- Probiotics and Fermented Foods
Chulalongkorn University
2022-2024
Center for Systems Biology
2023
The first publication demonstrating that major depressive disorder (MDD) is associated with alterations in the gut microbiota appeared 2008 (Maes et al., 2008). purpose of present study to delineate a) microbiome signature phenome depression, including suicidal behaviours (SB) and cognitive deficits; effects adverse childhood experiences (ACEs) recurrence illness index (ROI) on microbiome; lowered high-density lipoprotein cholesterol (HDLc). We determined isometric log-ratio abundances or...
Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in microbiota–gut–brain axis, as evidenced elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative bacteria, such Morganella morganii and Klebsiella Pneumoniae. The latter aberrations, which point increased gut permeability (leaky gut), are linked activated neuro-immune oxidative pathways MDD. To delineate profile composition microbiome Thai patients...
Abstract The first publication demonstrating that major depressive disorder (MDD) is associated with alterations in the gut microbiota appeared 2008 (Maes et al., 2008). purpose of present study to delineate a) microbiome signature phenome depression, including suicidal behaviours and cognitive deficits; effects adverse childhood experiences (ACE) recurrence illness index (ROI) on microbiome; lowered high-density lipoprotein cholesterol (HDLc). We determined isometric log-ratio abundances or...
Ribosomal RNA gene amplicon sequencing is commonly used to evaluate microbiome profiles in health and disease document the impact of interventional treatments. Nanopore attractive since it can provide greater classification at species level. However, optimized protocols target marker genes for bacterial fungal profiling are needed. To achieve an increased taxonomic resolution, we developed extraction full-length PCR-based approaches using sequencing. Three lysis conditions were applied a...
Abstract The most frequent intestinal helminth infections in humans are attributed to Ascaris lumbricoides , and there concerns over the anthelminthic resistance of this species. gut microbiota has essential roles host physiology. Therefore, discovering host-parasite–microbiota interactions could help develop alternative helminthiasis treatments. Additionally, these modulated by functional metabolites that can reveal mechanisms infection disease progression. Thus, we aimed investigate...
The environment has an important effect on the gut microbiota-an essential part of host's health-and is strongly influenced by dietary pattern host as these together shape composition and functionality microbiota in humans other animals. This study compared Macaca fascicularis M. f. aurea mangrove island populations using 16S rRNA gene sequencing a nanopore platform to investigate and/or diet. results revealed that received anthropogenic food exhibited higher richness evenness than different...
Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting pathogenesis, but study limited. present explored association between gut-pharyngeal and stages in cynomolgus macaques using full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. total of 71 divided into (-) control, (+) groups. differential...
End-stage kidney disease (ESKD) is the final stage of chronic (CKD), in which long-term damage has been caused to kidneys extent that they are no longer able filter blood waste and extra fluid. Peritoneal dialysis (PD) one treatments remove products from through peritoneum can improve quality life for patients with ESKD. However, PD-associated peritonitis an important complication contributes mortality patients, detection bacterial pathogens associated a high culture-negative rate. The...
Ribosomal RNA gene amplicon sequencing is commonly used to evaluate microbiome profiles in health and disease document the impact of interventional treatments. Nanopore attractive since it can provide greater classification at species level. However, optimised protocols target marker genes for bacterial fungal profiling are needed. To achieve an increased taxonomic resolution, we developed extraction full-length PCR-based approaches using sequencing. Three lysis conditions were applied a...
Coronavirus disease 2019 (COVID-19) is a worldwide pandemic infectious caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). World Health Organization (WHO) has defined the viral variants of concern (VOC) which cause more disease, higher transmissibility, and reduced vaccine efficacy. In this study, "Nano COVID-19" workflow based on Oxford nanopore sequencing full-length spike gene combined with flexible data analysis options was developed to identify SARS-CoV-2 VOCs. The...
Breast cancer is a leading cause of cancer‑related deaths worldwide. Moreover, standard treatments are limited, so new alternative required. Thai traditional formulary medicine (TTFM) utilizes certain herbs to treat different diseases due their dominant properties including anti‑fungal, anti‑bacterial, antigenotoxic, anti‑inflammatory and anti‑cancer actions. However, very little known about the TTFM against breast cells underlying molecular mechanism has not been elucidated. Therefore,...
The epidermal growth factor receptor (EGFR) mutation is a widely prevalent oncogene driver in non-small cell lung cancer (NSCLC) East Asia. detection of EGFR mutations standard biomarker test performed routinely patients with NSCLC for the selection targeted therapy. Here, our objective was to develop portable new technique detecting (19Del, T790M, and L858R) based on Nanopore sequencing.
Abstract Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in microbiota-gut-brain axis, as evidenced elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative bacteria, such Morganella morganii and Klebsiella Pneumoniae . The latter aberrations, which point increased gut permeability (leaky gut), are linked activated neuro-immune oxidative pathways MDD. To delineate profile composition microbiome Thai...
Abstract Sex hormone levels affect the composition of vaginal flora. Changes in microbiome individual health. Among transgender men (TM) who receive gender-affirming treatment, there can be a change causing adverse effects that are neglected by both individuals and healthcare professionals. This study aims to understand influence testosterone on microbiome. prospective was conducted at King Chulalongkorn Memorial Hospital. All TM had not yet received were included. High-throughput sequencing...
Abstract Ribosomal RNA gene amplicon sequencing is commonly used to evaluate microbiome profiles in health and disease document the impact of interventional treatments. Long-read nanopore attractive since it can provide greater classification at species level. However, optimised protocols target marker genes for bacterial fungal profiling are needed. To achieve an increased taxonomic resolution, we developed extraction long-amplicon PCR-based approaches using Nanopore sequencing. Three...
Ribosomal RNA gene amplicon sequencing is commonly used to evaluate microbiome profiles in health and disease document the impact of interventional treatments. Nanopore attractive since it can provide greater classification at species level. However, optimised protocols target marker genes for bacterial fungal profiling are needed. To achieve an increased taxonomic resolution, we developed extraction full-length PCR-based approaches using sequencing. Three lysis conditions were applied a...