A5060310111- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- interferon and immune responses
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Burkholderia infections and melioidosis
- SARS-CoV-2 detection and testing
- Immunodeficiency and Autoimmune Disorders
- Allelopathy and phytotoxic interactions
- Infections and bacterial resistance
- Clostridium difficile and Clostridium perfringens research
- Infectious Diseases and Tuberculosis
- Influenza Virus Research Studies
- Antibiotic Use and Resistance
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Chemical Looping and Thermochemical Processes
- Corporate Taxation and Avoidance
- Urinary Tract Infections Management
- Diagnosis and treatment of tuberculosis
- Phytoplasmas and Hemiptera pathogens
- Gut microbiota and health
- Systemic Lupus Erythematosus Research
Siriraj Hospital
2014-2024
Mahidol University
2014-2024
Mahidol Oxford Tropical Medicine Research Unit
2016-2020
In southeast Asia, antibiotic prescription in febrile patients attending primary care is common, and a probable contributor to the high burden of antimicrobial resistance. The objective this trial was explore whether C-reactive protein (CRP) testing at point could rationalise care, comparing two proposed thresholds classify CRP concentrations as low or guide treatment.
Background Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains which express a pseudomallei-like capsular polysaccharide (BTCV), also commonly found in environment Southeast Asia but considered non-pathogenic. The aim study was to determine distribution thailandensis its variant Thailand investigate whether presence associated with serological response pseudomallei. Methodology/principal findings We evaluated 61...
ABSTRACT This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes 1,414 culture-positive M. isolates from 1,282 pulmonary (PTB) 132 extrapulmonary TB (EPTB) patients collected 1995 to 2011 were characterized. Among eight SNP cluster groups (SCG), SCG2 (44.1%), which included Beijing (BJ) genotype, SCG1 (39.4%), an East African Indian...
The successful culture of Leptospira spp. from the environment is challenging. Here, we optimized isolation water samples spiked with different species and initial concentrations this organism. time periods between sampling process were varied (0, 2, 4 weeks). Bacterial cultures observed under a microscope, graded for cell density, weekly, 12 weeks. Most pathogenic difficult to all conditions. All conditions novel subclade P1 positive within 2 For P2, storing weeks prior resulted in more...
Abstract Tuberculosis is a global public health problem with emergence of multidrug-resistant infections. Previous epidemiological studies tuberculosis in Thailand have identified clonal outbreak strain Mycobacterium the Kanchanaburi province, designated “MKR superspreader”, and this particular later was found to also spread other regions. In study, we elucidated its biology through RNA-Seq analyses set genes involved cholesterol degradation be up-regulated MKR during macrophage cell...
Abstract The detection and management of Mycobacterium tuberculosis complex (MTBC) infection, the causative agent (TB), in macaques, including cynomolgus macaques ( Macaca fascicularis ), are significant concern research regions where coexist with humans or other animals. This study explored utility Xpert MTB/RIF Ultra assay, a widely adopted molecular diagnostic tool to diagnose (TB) humans, detect DNA from clinical samples obtained macaques. investigation involved comprehensive comparative...
Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc), which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting pathogenesis, but study limited. present explored association between gut-pharyngeal and stages in cynomolgus macaques using full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. total of 71 divided into (-) control, (+) groups. differential...
Tuberculosis disease (TB), caused by
A multipurpose high-throughput genotyping tool for the assessment of recent epidemiological data and evolutional pattern in Mycobacterium tuberculosis complex (MTBC) clinical isolates was developed this study. To facilitate processing, 51 highly informative single nucleotide polymorphisms (SNPs) were selected discriminating clinically most relevant MTBC species M. into its principle genetic groups (PGGs) SNP cluster (SCGs). Because high flexibility DigiTag2 assay, identical protocol DNA...
Background Culture is the gold standard for detection of environmental B. pseudomallei. In general, soil specimens are cultured in enrichment broth 2 days, and then culture streaked on an agar plate incubated further 7 days. However, identifying pseudomallei plates among other microbes requires expertise experience. Here, we evaluate a lateral flow immunoassay (LFI) developed to detect capsular polysaccharide (CPS) clinical samples as tool samples. Methodology/Principal Findings First,...
Abstract Tuberculosis (TB) is the first infectious disease to be screened-out from specified pathogen-free cynomolgus macaques ( Macaca fascicularis ; Mf) using in human pharmaceutical testing. Being either latent or active stage after exposure Mycobacterium tuberculosis complex (MTBC), monkey gamma-interferon release assay (mIGRA) was previously introduced for early TB detection Mf. However, a high number of indeterminate cases were unexpectedly encountered. The main reasons mitogen...
Tuberculosis (TB) is the first infectious disease to be screened-out from specified pathogen-free cynomolgus macaques ( Macaca fascicularis ; Mf) using in human pharmaceutical testing. Being either latent or active stage after exposure Mycobacterium tuberculosis complex (MTBC), monkey gamma-interferon release assay (mIGRA) was previously introduced for early TB detection. However, a notable incidence of indeterminate results observed. In this study, we compared two positive mitogen...