A5019975803- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Protein Structure and Dynamics
- SARS-CoV-2 detection and testing
- Machine Learning in Bioinformatics
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- COVID-19 epidemiological studies
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- Enzyme Structure and Function
- RNA Research and Splicing
- vaccines and immunoinformatics approaches
- Viral Infections and Outbreaks Research
- Food Allergy and Anaphylaxis Research
- Cellular transport and secretion
- RNA modifications and cancer
- Virology and Viral Diseases
Agency for Science, Technology and Research
2015-2024
Bioinformatics Institute
2015-2024
National University of Singapore
2012-2024
A*STAR Graduate Academy
2016-2024
National Centre for Infectious Diseases
2019-2023
Ministry of Health
2013-2023
Nanyang Technological University
2010-2022
Sengkang General Hospital
2020
SingHealth
2020
University of Amsterdam
2019
GISAID is a global data science initiative and the primary source of genomic associated metadata all influenza viruses, Respiratory Syncytial Virus (RSV) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), pandemic causing disease 2019 (COVID-19).GISAID's publicly accessible sharing platform enables collaboration over 42,000 participating researchers from 198 nations generators 3,500 institutions across globe.Since first wholegenome sequences were made available by China CDC...
Three clusters of coronavirus disease 2019 (COVID-19) linked to a tour group from China, company conference, and church were identified in Singapore February, 2020.We gathered epidemiological clinical data individuals with confirmed COVID-19, via interviews inpatient medical records, we did field investigations assess interactions possible modes transmission severe acute respiratory syndrome 2 (SARS-CoV-2). Open source reports obtained for overseas cases. We reported the median (IQR)...
Abstract Background The impact of SARS-CoV-2 variants concern (VOCs) on disease severity is unclear. In this retrospective study, we compared the outcomes patients infected with B.1.1.7, B.1.351, and B.1.617.2 wild-type strains from early 2020. Methods National surveillance data January to May 2021 were obtained in relation VOCs explored. Detailed patient-level all VOC infection admitted our center between December 2020 analyzed. Clinical a cohort 846 April Results A total 829 Singapore...
During the SARS epidemic in 2003/2004, a number of deletions were observed ORF8 SARS-CoV, and eventually deletion variants became predominant, leading to hypothesis that was an evolutionary hot spot for adaptation SARS-CoV humans. However, due successful control epidemic, importance these epidemiological fitness humans could not be established. The emergence multiple SARS-CoV-2 strains with deletions, combined evidence robust immune response ORF8, suggests lack may assist host evasion. In...
We propose a novel, non-discriminatory classification of monkeypox virus diversity. Together with the World Health Organization, we named three clades (I, IIa and IIb) in order detection. Within IIb, cause current global outbreak, identified multiple lineages (A.1, A.2, A.1.1 B.1) to support real-time genomic surveillance.
Single nucleotide variants (SNVs) are, together with copy number variation, the primary source of variation in human genome and are associated phenotypic such as altered response to drug treatment susceptibility disease. Linking structural effects non-synonymous SNVs functional outcomes is a major issue bioinformatics. The SNPeffect database (http://snpeffect.switchlab.org) uses sequence- structure-based bioinformatics tools predict effect protein-coding on phenotype proteins. It integrates...
We show the distribution of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) genetic clades over time and between countries outline potential genomic surveillance objectives. applied three nomenclature systems to all sequence data from World Health Organization European Region available until 10 July 2020. highlight importance real-time sequencing dissemination in a pandemic situation, compare nomenclatures lay foundation for future SARS-CoV-2.
A complex interplay of viral, host, and ecological factors shapes the spatio-temporal incidence evolution human influenza viruses. Although considerable attention has been paid to viruses, a lack equivalent data means that an integrated evolutionary epidemiological framework until now not available for B despite their significant disease burden. Through analysis over 900 full genomes from collection more than 26,000 strains Australia New Zealand, we reveal fundamental differences in...
Malassezia is a unique lipophilic genus in class Malasseziomycetes Ustilaginomycotina, (Basidiomycota, fungi) that otherwise consists almost exclusively of plant pathogens. are typically isolated from warm-blooded animals, dominant members the human skin mycobiome and associated with common disorders. To characterize genetic basis phenotypes spp., we sequenced genomes all 14 accepted species used comparative genomics against broad panel fungal to comprehensively identify distinct features...
Primary congenital glaucoma (PCG) is a devastating eye disease and an important cause of childhood blindness worldwide. In PCG, defects in the anterior chamber aqueous humor outflow structures result elevated intraocular pressure (IOP); however, genes molecular mechanisms involved etiology these have not been fully characterized. Previously, we observed PCG-like phenotypes transgenic mice that lack functional angiopoietin-TEK signaling. Herein, identified rare TEK variants 10 189 unrelated...
Abstract Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate lipid biosynthesis and adipogenesis by controlling the expression several enzymes required for cholesterol, fatty acid, triacylglycerol phospholipid synthesis. In vertebrates, SREBP activation is mainly controlled complex well-characterized feedback mechanism mediated crucial bio-product SREBP-activated mevalonate pathway. this work, we identified acto-myosin contractility...
This paper aims at synthesizing multiple realistic-looking retinal (or neuronal) images from an unseen tubular structured annotation that contains the binary vessel morphology. The generated phantoms are expected to preserve same structure, and resemble visual appearance of training images. Inspired by recent progresses in generative adversarial nets (GANs) as well image style transfer, our approach enjoys several advantages. It works with a small set few 10 examples, which is common...
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that robust viral genome mutations and temperature, produces results fast, can be applied directly on nasopharyngeal (NP) specimens without RNA purification, incorporates human internal control within same reaction. Specifically, show use an engineered AsCas12a enzyme enables detection wildtype mutated SARS-CoV-2 allows us perform...
Background: The impact of SARS-CoV-2 variants concern (VOCs) on disease severity is unclear. In this retrospective cohort study, we compared outcomes patients infected with B.1.1.7, B.1.351, and B.1.617.2 those wild-type strains from early 2020.Methods: National surveillance data 1-January-2021 to 22-May-2021 were obtained the Ministry Health, in relation VOC explored. Detailed patient level all infection admitted our centre between 20-December-2020 12-May-2021 analysed. Outcomes a 846...
Proteins in food and personal care products can pose a risk for an immediate immunoglobulin E (IgE)-mediated allergic response. Bioinformatic tools assist to predict investigate the allergenic potential of proteins. Here we present AllerCatPro 2.0, web server that be used protein allergenicity with better accuracy than other computational methods new features help assessors making informed decisions. 2.0 predicts similarity between input proteins using both their amino acid sequences...