A5008256216- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Prion Diseases and Protein Misfolding
- Machine Learning in Bioinformatics
- Estrogen and related hormone effects
- Chemical Synthesis and Analysis
- Steroid Chemistry and Biochemistry
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Monoclonal and Polyclonal Antibodies Research
- Bioinformatics and Genomic Networks
- Computational Drug Discovery Methods
- RNA Research and Splicing
- X-ray Diffraction in Crystallography
- Amyotrophic Lateral Sclerosis Research
- Cellular transport and secretion
- Proteins in Food Systems
- Crystallization and Solubility Studies
- Agriculture and Rural Development Research
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
KU Leuven
2016-2025
VIB-KU Leuven Center for Brain & Disease Research
2016-2025
Switch
2008-2024
Rega Institute for Medical Research
2024
Institut de Radioprotection et de Sûreté Nucléaire
2023-2024
Vlaams Instituut voor Biotechnologie
2006-2021
VIB-KU Leuven Center for Microbiology
2021
Concordia University
2021
Institut de Recherche Interdisciplinaire en Sciences Sociales
2009-2017
Center for Research and Interdisciplinarity
2009-2017
Liquid-liquid phase separation (LLPS) of RNA-binding proteins plays an important role in the formation multiple membrane-less organelles involved RNA metabolism, including stress granules. Defects granule homeostasis constitute a cornerstone ALS/FTLD pathogenesis. Polar residues (tyrosine and glutamine) have been previously demonstrated to be critical for ALS-linked proteins. We now identify active arginine-rich domains these separations. Moreover, dipeptide repeats (DPRs) derived from...
The empirical force field Fold-X was developed previously to allow rapid free energy calculations in proteins. Here, we present an enhanced version of the allowing prediction position structural water molecules and metal ions, together called single atom ligands. picks up 76% found interact with two or more polar atoms proteins high-resolution crystal structures predicts their within 0.8 Å on average. ion-binding sites have success rates between 90% 97% depending metal, overall standard...
A graphical user interface for the FoldX protein design program has been developed as a plugin YASARA molecular graphics suite. The most prominent commands such free energy difference upon mutagenesis and interaction calculations can now be run entirely via windowed menu system results are immediately shown on screen.The is written in Python freely available download at http://foldxyasara.switchlab.org/ supported Linux, MacOSX MS Windows.
Abstract Hexanucleotide repeat expansions in C9orf72 are the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal degeneration (FTD) (c9ALS/FTD). Unconventional translation these repeats produces dipeptide proteins (DPRs) that may neurodegeneration. We performed a modifier screen Drosophila discovered critical role for importins exportins, Ran-GTP cycle regulators, nuclear pore components arginine methylases mediating DPR toxicity. These findings provide evidence an...
Abnormal calcium signaling is a central pathological component of Alzheimer's disease (AD). Here, we describe the identification class compounds called ReS19-T, which are able to restore homeostasis in cell-based models tau pathology. Aberrant accumulation leads uncontrolled activation store-operated channels (SOCCs) by remodeling septin filaments at cell cortex. Binding ReS19-T septins restores filament assembly state and restrains entry through SOCCs. In amyloid-β tau-driven mouse disease,...
Recalcitrant bacterial infections can be caused by various types of dormant bacteria, including persisters and viable but nonculturable (VBNC) cells. Despite their clinical importance, we know fairly little about dormancy development recovery. Previously, established a correlation between protein aggregation in Escherichia coli. Here, present further support for direct relationship both. Our experiments demonstrate that aggregates progressively sequester proteins involved energy production,...
Since the reformulation of amyloid cascade hypothesis to focus on oligomeric aggregates beta as prime toxic species causing Alzheimer's disease, many researchers refocused detecting a specific molecular assembly defined size thatis main trigger disease. The result has been identification host assemblies containing from two up hundred molecules peptide, which were all found impair memory formation in mice. This clearly demonstrates that is insufficient define toxicity and peptide conformation...
<h3>Objective:</h3> To assess the genetic contribution of <i>TBK1</i>, a gene implicated in amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and FTD-ALS, Belgian FTD ALS patient cohorts containing significant part genetically unresolved patients. <h3>Methods:</h3> We sequenced <i>TBK1</i> hospital-based cohort 482 unrelated patients with FTD-ALS 147 an extended family DR158. followed up mutation carriers by segregation studies, transcript protein expression analysis,...