A5069051474- Malaria Research and Control
- Mosquito-borne diseases and control
- Drug-Induced Hepatotoxicity and Protection
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Infectious Encephalopathies and Encephalitis
- HIV/AIDS drug development and treatment
- Research on Leishmaniasis Studies
- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Vector-borne infectious diseases
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Travel-related health issues
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Trypanosoma species research and implications
- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Biochemical and Structural Characterization
- Blood properties and coagulation
- Antibiotic Resistance in Bacteria
Mahidol University
2011-2024
Mahidol Oxford Tropical Medicine Research Unit
2011-2024
Hospital for Tropical Diseases
2008-2016
Drugs for Neglected Diseases Initiative
2010
Universiti Sains Malaysia
2010
Bangkok Hospital
2007-2010
University of Oxford
2010
Asia Foundation
2008
University Health Network
2007
University of Toronto
2007
Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at community level useful programs to control disease. The aim present work was develop a simple, inexpensive molecular Plasmodium falciparum.Blood collected from controls (n = 100) and patients diagnosed with falciparum infection 102), who were recruited study. Heat-treated blood samples tested by loop-mediated isothermal amplification (LAMP) assay P....
Background Limited tools exist to identify which individuals infected with Plasmodium falciparum are at risk of developing serious complications such as cerebral malaria (CM). The objective this study was assess serum biomarkers that differentiate between CM and non-CM, the long-term goal a clinically informative prognostic test for severe malaria. Methodology/Principal Findings Based on hypothesis endothelial activation blood-brain-barrier dysfunction contribute pathogenesis, we examined...
Abstract Background Severe and cerebral malaria are associated with endothelial activation. Angiopoietin-1 (ANG-1) angiopoietin-2 (ANG-2) major regulators of activation integrity. The aim this study was to investigate the clinical utility whole blood angiopoietin (ANG) levels as biomarkers disease severity in Plasmodium falciparum malaria. Methods ANG examined Thai patients distinguish (CM; n = 87) severe (non-cerebral) (SM; 36) from uncomplicated (UM; 70). Comparative statistics reported...
Plasmodium vivax causes debilitating but usually non-lethal malaria in most of Asia and South America. Prevention relapse after otherwise effective therapy for the acute attack requires a standard daily dose primaquine administered over 14 days. This regimen has < 90% efficacy Thailand, is widely regarded as ineffective because poor compliance relatively long duration dosing. We evaluated efficacy, safety, tolerability alternative dosing regimens combined with artesunate among 399 Thai...
Despite the use of potent antimalarial drugs, fatality rate associated with severe malaria remains high. Adjunctive therapies that target immunopathological responses to infection may decrease mortality malaria. We hypothesized peroxisome proliferator-activated receptor gamma agonists (eg, rosiglitazone) would modulate host's innate immune response and improve outcome.In a randomized, double-blind, placebo-controlled, phase I/II trial treatment for acquired in Thailand, we investigated...
Background. Drug-resistant Plasmodium falciparum malaria necessitates development of novel drugs for treatment. The present study assessed the efficacy and safety 3 dose levels arterolane (RBx 11160), a synthetic trioxolane, treatment acute uncomplicated malaria. Methods. In this randomized, double-blind, multicenter, parallel-group, dose-finding, phase II trial, 230 patients from 4 centers in Thailand, India, Tanzania (mainland Zanzibar) received either 50 mg ( n = 78), 100 76), or 200 76)...
Because antimalarial drug resistance is spreading, there an urgent need for new combination treatments malaria, which kills >1 million people every year. Azithromycin a macrolide antibiotic that particularly attractive as because of its safety in children and the extensive experience with use during pregnancy.We undertook randomized, controlled, 28-day inpatient trial involving patients acute, uncomplicated Plasmodium falciparum malaria. We compared efficacy 2 azithromycin-artesunate...
Background. Artemisinin-based combination therapy is the first-line treatment for uncomplicated falciparum malaria. This study assessed antimalarial efficacy and safety of a 150 mg arterolane maleate 750 piperaquine phosphate (AM-PQP) in comparison to Coartem (artemether lumefantrine) patients with acute P. Methods. In this open-label, randomized, multicentric, parallel group clinical trial, 240 were randomized receive AM-PQP (160 patients) or (80 patients). Patients monoinfection initial...
Summary Introduction: While hyperparasitemia is considered an important indicator for the development of severe malaria, there currently no consensus on quantitative definition hyperparasitemia. This study was conducted to establish a cutoff point peripheral parasitemia among patients with Plasmodium falciparum define malaria. Methods: The clinical presentations 200 uncomplicated P. and 189 patients, admitted Hospital Tropical Diseases, Faculty Medicine, Mahidol University, Bangkok,...
ABSTRACT Azithromycin when used in combination with faster-acting antimalarials has proven efficacious treating Plasmodium falciparum malaria phase 2 clinical trials. The aim of this study was to establish optimal ratios for azithromycin either dihydroartemisinin or quinine, determine the correlates vitro drug sensitivity these compounds, and assess cross-sensitivity patterns. Seventy-three fresh P. isolates originating from patients western border regions Thailand were successfully tested...
Galectin-9 (Gal-9) is a β-galactoside-binding lectin that interacts with sugar moieties on glycoproteins and glycolipids of cells pathogens. Gal-9 known as an immune modulator induces cell death via interaction T immunoglobulin mucin domain-3 (Tim3), co-inhibitory receptor, it inhibits production several pro-inflammatory cytokines (TNF, IL-6 IL-1α) enhances IL-10. To understand the pathology malaria, in plasma was measured. Plasma samples clinical parameters were obtained from 50 acute...
BackgroundDB289 is the orally active prodrug of diamidine DB75, which was developed for treatment human African trypanosomiasis MethodsWe tested safety and efficacy DB289 Plasmodium vivax acute, uncomplicated P. falciparum infections in an open-label pilot study at Hospital Tropical Diseases Bangkok. Nine patients with 23 were admitted treated 100 mg given twice a day 5 days followed 28 days. Patients also primaquine on 10–23 ResultsAll cleared parasites by 7, mean±SD clearance time 43±41 h....
Artemisinin-based combination therapy (ACT) is currently promoted as a strategy for treating both uncomplicated and severe falciparum malaria, targeting asexual blood-stage Plasmodium parasites. However, the effect of ACT on sexual-stage parasites remains controversial. To determine clearance P. from 342 uncomplicated, 217 severe, adult malaria cases, we reviewed followed peripheral blood 4 wk after starting ACT. All patients presented with sexual stage admission, were treated...
A new fixed-dose artesunate (AS)-mefloquine (MQ) was assessed in adults hospitalized for 28 days with uncomplicated drug-resistant falciparum malaria. The patients (n = 25/arm) were treated (i) two tablets (AS-MQ arm; 100 mg AS-200 MQ/tablet) daily 3 (days 0, 1, and 2) or (ii) nonfixed AS (AS-plus-MQ 4 mg/kg of body weight/day days) plus MQ (15 on day 1 10 2), dosed by weight. Clinical laboratory electrocardiogram (ECG), adverse events (AEs), efficacy, pharmacokinetic parameters over days....
Abstract Background Malaria parasites and their products can activate a specific immune response by stimulating cytokine production in the host’s cells. Transcription nuclear factor kappa B (NF-κB) is an important regulator for control of many pro-inflammatory genes, such as interleukin-1 (IL-1) tumor necrosis (TNF). The activation expression NF-κB p65 peripheral blood mononuclear cells (PBMCs) malaria patients were investigated correlated with levels IL-10 TNF to study nature its linkage...
BackgroundRabies has become a forgotten and neglected disease in Japan. In 2006, there was slight increase social awareness of rabies when Japan had two reported cases human rabies, originating the Philippines. Although number Japanese either traveling or living other Asian countries been increasing, exact risk this population contracting is unknown. Thus, study utilized questionnaire to investigate incidence exposure, as well knowledge, attitude, practice toward prevention among expatriates...
We conducted a study to compare the safety and tolerability of anti-relapse drugs elubaquine primaquine against Plasmodium vivax malaria. After standard therapy with chloroquine, 30 mg/kg given over 3 days, 141 patients P. infection were randomized receive or elubaquine. The 2 treatment regimens mg once daily for 7 days (group A, n = 71), 25 B, 70). All cleared parasitemia within after chloroquine treatment. Among treated primaquine, one patient relapsed on day 26; no relapse occurred...
Liver function tests were performed in 61 vivax, 54 malariae and 15 ovale malaria patients who admitted to Bangkok Hospital for Tropical Diseases between 2001 2004. The objective of the study was evaluate changes hepatic biochemical indices before after treatment with artemisinin derivatives. On admission prior treatment, dysfunction found among 3 groups. Serum liver physical examinations weekly during 28-day follow-up period. Initially elevated serum bilirubin diminished albumin returned...
Chloroquine remains the drug of choice for treatment vivax malaria in Thailand. Mixed infections falciparum and are also common South-East Asia. Laboratory confirmation species is not generally available. This study aimed to find alternative regimens treating both by using antimalarial drugs. From June 2004 May 2005, 98 patients with Plasmodium were randomly treated either artemether-lumefantrine (n = 47) or chloroquine 51). Both treatments followed 15 mg primaquine over 14 days. Adverse...
Most trials of antimalarials occur in areas which reinfections are possible. For Plasmodium falciparum, distinguished from recrudescences by polymerase chain reaction analysis 3 polymorphic genes. However, the validity this approach has never been rigorously tested. We tested for misclassification 6 patients clinical Thailand and Cambodia who were classified as being reinfected standard protocol. Using heteroduplex tracking assays direct DNA sequencing, we found that 5 (83%) misclassified....
We reviewed the records of 1,175 patients with uncomplicated Plasmodium falciparum malaria to determine prevalence gametocytemia. All were admitted and received artemisinin combination therapy. Blood films checked daily until discharge. Circulating gametocytes observed in 240 (20.2%) most cases (222 240, 92.5%) gametocytemia was detected during first 24 hours after admission. Gametocytes seen 174 on admission, at 12 hours, hours. The longest interval between admission appearance 192 median...