A5063465178- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Drug-Induced Hepatotoxicity and Protection
- HIV/AIDS drug development and treatment
- Travel-related health issues
- Parasites and Host Interactions
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Global Maternal and Child Health
- Pharmacological Effects and Toxicity Studies
- Hemoglobinopathies and Related Disorders
- Research on Leishmaniasis Studies
- Antibiotics Pharmacokinetics and Efficacy
- Trypanosoma species research and implications
- Child Nutrition and Water Access
- Complement system in diseases
- Tuberculosis Research and Epidemiology
- Genetic diversity and population structure
- Pneumocystis jirovecii pneumonia detection and treatment
- Vector-borne infectious diseases
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Evolution and Genetic Dynamics
- Lignin and Wood Chemistry
Karolinska Institutet
2016-2025
Sahlgrenska University Hospital
2023-2024
University of Gothenburg
2022-2024
Uppsala University
2018-2022
Karolinska University Hospital
2006-2018
Université des Sciences, des Techniques et des Technologies de Bamako
2018
Lusíada University of Lisbon
2018
University of Lisbon
2018
University of Minho
2018
National Institute of Economic Research
2017
Background The Roll Back Malaria strategy recommends a combination of interventions for malaria control. Zanzibar implemented artemisinin-based therapy (ACT) uncomplicated in late 2003 and long-lasting insecticidal nets (LLINs) from early 2006. ACT is provided free charge to all patients, while LLINs are distributed children under age 5 y ("under five") pregnant women. We investigated temporal trends Plasmodium falciparum prevalence malaria-related health parameters following the...
Artemisinin derivative-based combination therapy is expected to suppress the development of Plasmodium falciparum drug resistance in Africa. We have performed an artemether-lumefantrine (Coartem; Novartis) follow-up clinical trial Zanzibar, which pfcrt K76T and pfmdr1 N86Y frequencies were determined before administration all recurrent parasites during a period 42 days. A significant increase 86N was observed after exposure drug. This points as potential marker lumefantrine vivo, while...
Adequate clinical and parasitologic cure by artemisinin combination therapies relies on the component partner drug. Polymorphisms in Plasmodium falciparum chloroquine resistance transporter ( pfcrt ) P. multidrug 1 pfmdr1 genes are associated with decreased sensitivity to amodiaquine lumefantrine, but effects of these polymorphisms therapeutic responses artesunate-amodiaquine (ASAQ) artemether-lumefantrine (AL) have not been clearly defined. Individual patient data from 31 trials were...
Abstract Malaria infections occurring below the limit of detection standard diagnostics are common in all endemic settings. However, key questions remain surrounding their contribution to sustaining transmission and whether they need be detected targeted achieve malaria elimination. In this study we analyse a range datasets quantify density, detectability, course infection infectiousness subpatent infections. Asymptomatically infected individuals have lower parasite densities on average low...
Amodiaquine (AQ) metabolism to N-desethylamodiaquine (DEAQ) is the principal route of disposition in humans. Using human liver microsomes and two sets recombinant cytochrome P450 isoforms (from lymphoblastoids yeast) we performed studies identify CYP isoform(s) involved AQ. CYP2C8 was main hepatic isoform that cleared AQ catalyzed formation DEAQ. The extrahepatic P450s, 1A1 1B1, also an unknown metabolite M2. K(m) V(max) values for N-desethylation were 1.2 microM 2.6 pmol/min/pmol CYP2C8,...
Monolayers of human erythrocytes (E) infected with Plasmodium falciparum were briefly fixed 1% glutaraldehyde and air dried. They then exposed to sera from patients P. malaria or donors immune this parasite tested in an indirect immunofluorescence assay (IFA). Parasites E made visible by counterstaining ethidium bromide. Immunofluorescence (IF) was restricted the surface E. No antibody binding detected unless dried, suggesting that relevant antigens not available on outer layers surface....
Plasmodium falciparum is the major cause of malaria morbidity and mortality in world. Biologic antigenic diversity a characteristic this parasite infections can consist several genetically diverse parasites. The daily dynamics these subpopulations were investigated asymptomatic children rural Tanzania. Fingerprick blood samples collected on 14 consecutive days from 20 children. Parasite densities detected by light microscopy genotyping P. was done using nested polymerase chain reaction (PCR)...
Artemether-lumefantrine (AL) is a major and highly effective artemisinin-based combination therapy that becoming increasingly important as new first-line against Plasmodium falciparum malaria. However, recrudescences occurring after AL treatment have been reported. Identification of drug-specific parasite determinants contribute to failures will provide tools for the detection surveillance resistance.The findings from 42-day follow-up efficacy trial in Tanzania compared with...
Background. This is the first clinical trial comparing efficacy of artesunate plus amodiaquine (ASAQ) and artemether-lumefantrine (AL)—the major artemisinin-based combination therapy (ACT) candidates for treatment malaria in Africa—that involved an extended, 42-day follow-up period, polymerase chain reaction–adjusted parasitological cure rates (PCR APCRs), systematic analyses genetic markers related to quinoline resistance. Methods. A total 408 children with uncomplicated Plasmodium...
Background The use of rapid diagnostic tests (RDTs) for Plasmodium falciparum malaria is being suggested to improve efficiency in peripheral health care settings Africa. Such improved diagnostics are critical minimize overuse and thereby delay development resistance artemisinin-based combination therapies (ACTs). Our objective was study the influence RDT-aided diagnosis on drug prescriptions, outcomes, costs primary settings. Methods Findings We conducted a cross-over validation clinical...
Summary Objective Artemether‐lumefantrine (AL), presently the most favoured combination therapy against uncomplicated Plasmodium falciparum malaria in Africa, has recently shown to select for pfmdr1 86N allele. The objective of this study was search selection other mutations potentially involved artemether‐lumefantrine tolerance and/or resistance, i.e. gene amplification, Y184F, S1034C, N1042D, D1246Y, pfcrt S163R and PfATP6 S769N. Methods above mentioned SNPs were analysed by...
IgG from a donor clinically immune to Plasmodium falciparum malaria strongly inhibited reinvasion in vitro of human erythrocytes by the parasite. When added monolayers glutaraldehyde-fixed and air-dried infected with parasite, this also displayed characteristic immunofluorescence restricted surface erythrocytes. Elution adsorbed such gave an antibody fraction that was 40 times more efficient inhibition assay (50% titer, less than 1 microgram/ml) original preparation. The major eluate...
Early diagnosis and prompt, effective treatment of uncomplicated malaria is critical to prevent severe disease, death transmission. We assessed the impact rapid diagnostic tests (RDTs) by community health workers (CHWs) on provision artemisinin-based combination therapy (ACT) outcome in fever patients.Twenty-two CHWs from five villages Kibaha District, a high-malaria transmission area Coast Region, Tanzania, were trained manage using RDT aided or clinical (CD) only. Each CHW was randomly...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLignin and Lignin-Carbohydrate ComplexesAnders. BjorkmanCite this: Ind. Eng. Chem. 1957, 49, 9, 1395–1398Publication Date (Print):September 1, 1957Publication History Published online1 May 2002Published inissue 1 September 1957https://pubs.acs.org/doi/10.1021/ie50573a040https://doi.org/10.1021/ie50573a040research-articleACS PublicationsRequest reuse permissionsArticle Views963Altmetric-Citations108LEARN ABOUT THESE METRICSArticle Views are the...
In Zanzibar, the Ministry of Health and partners accelerated malaria control from September 2003 onwards. The impact scale-up insecticide-treated nets (ITN), indoor-residual spraying (IRS) artemisinin-combination therapy (ACT) combined on burden was assessed at six out seven in-patient health facilities. Numbers outpatient inpatient cases deaths were compared between 2008 pre-intervention period 1999-2003. Reductions estimated by segmented log-linear regression, adjusting effect size for...
Multidrug-resistant Plasmodium falciparum is a major threat to global malaria control. Parasites develop resistance by gradually acquiring genetic polymorphisms that decrease drug susceptibility. The aim of this study was investigate the extent which parasites with different characteristics are able withstand individual blood concentrations.We analyzed 2 clinical trials assessed efficacy and effectiveness artemether-lumefantrine. As proof concept, we used measured day 7 lumefantrine...
Asymptomatic, low parasite density malaria infections are difficult to detect with currently available point-of-care diagnostics. This study piloted a loop-mediated isothermal amplification (LAMP) kit for field-friendly, high-throughput detection of asymptomatic during mass screening and treatment (MSAT) in Zanzibar, pre-elimination setting. Screening took place three known hotspot areas prior the short rains November. Finger-prick blood was taken by rapid diagnostic test (RDT) LAMP...
<b>Objectives</b> To examine the impact of use rapid diagnostic tests for malaria on prescribing antimicrobials, specifically antibiotics, acute febrile illness in Africa and Asia. <b>Design</b> Analysis<b></b>of nine preselected linked codesigned observational randomised studies (eight cluster or individually trials one study). <b>Setting</b> Public private healthcare settings, 2007-13, Afghanistan, Cameroon, Ghana, Nigeria, Tanzania, Uganda. <b>Participants</b> 522 480 children adults with...
Background. Seasonal increases in malaria continue hot spots Zanzibar. Mass screening and treatment (MSAT) may help reduce the reservoir of infection; however, it is unclear whether rapid diagnostic tests (RDTs) detect a sufficient proportion low-density infections to influence subsequent transmission.
Artemether-lumefantrine and artesunate-amodiaquine are used as first-line artemisinin-based combination therapies (ACTs) in west Africa. Pyronaridine-artesunate dihydroartemisinin-piperaquine potentially useful for diversification of ACTs this region, but further safety efficacy data required on malaria retreatment.We did a randomised, multicentre, open-label, longitudinal, controlled phase 3b/4 clinical trial at seven tertiary centres Burkina Faso, Guinea, Mali. Eligible participants first...