A5008682194- Antibiotic Resistance in Bacteria
- Syphilis Diagnosis and Treatment
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
- Mycobacterium research and diagnosis
- Machine Learning in Bioinformatics
- Bacterial biofilms and quorum sensing
- Reproductive tract infections research
- Gut microbiota and health
- Antibiotic Use and Resistance
- Enterobacteriaceae and Cronobacter Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Veterinary medicine and infectious diseases
- Clostridium difficile and Clostridium perfringens research
- Plant Virus Research Studies
- Parasitic Infections and Diagnostics
- Cancer-related gene regulation
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Rabies epidemiology and control
NC Department of Health and Human Services
2012-2025
North Central State College
2019-2022
North Carolina Division of Public Health
2009-2022
Public Health Department
1998-2022
Karolinska University Hospital
2007
University of North Carolina at Chapel Hill
1997-2006
Faculty of Public Health
2002
ABSTRACT The Norwalk virus (NV) capsid protein was expressed using Venezuelan equine encephalitis replicon particles (VRP-NV1). VRP-NV1 infection resulted in large numbers of recombinant NV-like that were primarily cell associated and indistinguishable from NV produced baculoviruses. Mutations located the N-terminal P1 domains ablated self-assembly mammalian cells.
Abstract In the United States, New Delhi metallo-beta-lactamase (NDM)-producing carbapenem-resistant Enterobacterales (CRE) are frequently associated with healthcare encounters. From September 2021 to 2022, 21 patients NDM-CRE identified from urine and without exposure were reported Centers for Disease Control Prevention. Isolates genetically similar healthcare-associated strains.
A colistin-resistant Salmonella enterica 4, [5],12:i:- sequence type (ST) 34 harbouring mcr-3.1 was recovered from a patient who travelled to China 2 weeks prior diarrhoea onset. Genomic analysis revealed the presence of gene located in globally disseminated IncHI2 plasmid, highlighting intercontinental dissemination S. ST34 pandemic clone.
International lineages, such as Salmonella Typhimurium sequence type (ST) 19, are most often associated with foodborne diseases and deaths in humans. In this study, we compared the whole-genome sequences of five S. strains belonging to ST19 recovered from clinical human stool samples North Carolina, United States. Overall, displayed multidrug-resistant profile, being resistance critically highly important antimicrobials including ampicillin, ticarcillin/clavulanic acid, streptomycin...
TnphoA mutagenesis was used to identify genes encoding exported proteins in a genomic DNA library of Treponema pallidum, the syphilis agent. The nucleotide sequence an open reading frame (tprJ) that encodes 755-amino acid protein with predicted molecular mass 81.1 kDa determined. deduced amino TprJ has homology major surface denticola, periodontal pathogen. Southern hybridization and analysis indicate tprJ is member polymorphic multigene family. RT-PCR data showed expressed treponemes during...
Kerstersia spp. are an unusual cause of human infections. We report the first known case bacteremia and sepsis due to gyiorum, in a patient with chronic lower-extremity ulcers, we review literature on this uncommon pathogen.
We investigated an outbreak initially attributed to norovirus; however, Clostridium perfringens toxicoinfection was subsequently confirmed. C. is underrecognized but frequently observed cause of food-borne disease outbreaks. This investigation illustrates the importance considering epidemiologic and laboratory data together when evaluating potential etiologic agents that might require unique control measures.
Abstract Background Historically, United States’ carbapenem-resistant Enterobacterales (CRE) surveillance and mechanism testing focused on three genera: Escherichia, Klebsiella, Enterobacter (EsKE); however, other genera can harbour mobile carbapenemases associated with CRE spread. Objectives From January through May 2018, we conducted a 10 state evaluation to assess the contribution of less common (LCG) carbapenemase-producing (CP) CRE. Methods State public health laboratories (SPHLs)...
The nucleotide sequence of a DNA adenine methyltransferase gene (dam) from Treponema pallidum has been determined. Southern blot analysis T. chromosomal indicated that this is present as single copy. dam encodes 303 amino acid protein whose deduced significant homology with (N6-adenine) methyltransferases. Dam can be assigned to group α methyltransferases based on the order nine conserved motifs are in protein. Digests performed isoschizomer restriction endonucleases (Sau3AI, DpnI, and MboI)...
AbstractTreponema pallidum, the agent of syphilis, is a pathogenic spirochete that has no known mechanisms genetic exchange and cannot be continuously cultivated in vitro. A probe based on nucleotide sequence T. pallidum cheA gene was used to screen genomic DNA library. treponemal region containing four open reading frames (orfs) identified. The proteins encoded by these orfs have significant homology with involved bacterial chemotaxis. been designated cheA, cheW, cheX, cheY. cheY genes were...
ABSTRACT The nucleotide sequence of the Treponema pallidum mcp2 gene was determined. encodes a 45.8-kDa protein whose deduced amino acid has significant homology with C-terminal region bacterial methyl-accepting chemotaxis proteins (MCPs). Mcp2 N terminus lacks hydrophobic transmembrane regions present in most MCPs. An fusion strongly reactive antibody (HC23) to highly conserved domain MCPs and rabbit syphilitic serum. Antibody HC23 reacted six T. proteins, including 45-kDa that may...
The nucleotide sequence of a methyl-accepting chemotaxis protein gene, mcpA, from Treponema denticola has been determined. mcpA gene encodes 729-amino acid whose deduced amino significant homology with several bacterial MCPs. T. McpA contains two N-terminal transmembrane regions and C-terminal putative methylation sequences that are separated by highly conserved signaling domain. organization these structural features is characteristic observed molecular mass the in vitro synthesized (76.0...
The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Treponema pallidum has been determined. Southern blot analysis T. chromosomal indicated that this is present as single copy. organization genes flanking the gyrB unique in comparison to other bacteria. encodes 637 amino acid protein whose deduced high degree homology with type-II topoisomerase ATPase subunits (GyrB and ParE). Five motifs, an ATP-binding site (Walker A), residues putatively interact ATP, are highly conserved...