A5003430807- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Syphilis Diagnosis and Treatment
- Electron and X-Ray Spectroscopy Techniques
- HER2/EGFR in Cancer Research
- Bacterial Genetics and Biotechnology
- Lung Cancer Treatments and Mutations
- RNA regulation and disease
- Reproductive tract infections research
- Characterization and Applications of Magnetic Nanoparticles
- Machine Learning in Materials Science
- Head and Neck Cancer Studies
- RNA Interference and Gene Delivery
- Protease and Inhibitor Mechanisms
- Cancer survivorship and care
- Neurogenetic and Muscular Disorders Research
- Epigenetics and DNA Methylation
- Legume Nitrogen Fixing Symbiosis
- Gold and Silver Nanoparticles Synthesis and Applications
- Hematopoietic Stem Cell Transplantation
- Nanoparticle-Based Drug Delivery
- Acute Myeloid Leukemia Research
Research for Equity And Community Health Trust
2024
Realistic Education in Action Coalition to Foster Health
2024
Durham University
2021-2024
University of Colorado Anschutz Medical Campus
2018-2024
Thermo Fisher Scientific (United States)
2022
University of Chicago
2012-2015
MRC Clinical Trials Unit at UCL
2014
University College London
2014
University of North Carolina at Chapel Hill
1996-1997
North Carolina Division of Public Health
1996
Importance Numerous studies show that early palliative care improves quality of life and other key outcomes in patients with advanced cancer their caregivers, although most lack access to this evidence-based model care. Objective To evaluate whether delivering via secure video vs in-person visits has an equivalent effect on non–small cell lung (NSCLC). Design, Setting, Participants Randomized, multisite, comparative effectiveness trial from June 14, 2018, May 4, 2023, at 22 US centers among...
Transposon mutants of Agrobacterium tumefaciens which were avirulent and unable to attach plant cells isolated described previously. A clone from a library DNA was able complement these chromosomal att identified. Tn3HoHo1 insertions in this made used replace the wild-type genes bacterial chromosome by marker exchange. The resulting showed either no or very much reduced attachment carrot suspension culture cells. We sequenced 10-kb region found putative operon containing nine open reading...
Guidelines recommend lopinavir/ritonavir (LPV/r) as first- and second-line therapy for young older HIV-infected children, respectively. Available formulations have limitations making their widespread use complex.An open-label comparative bioavailability (randomized crossover) study compared a novel twice-daily minitab sprinkle formulation (40 mg/10 mg, Cipla Pharmaceuticals) versus innovator syrup in Ugandan infants aged 3 to <12 months (cohort A) children 1-4 years B) tablets (100/25 mg) 4...
Abstract The Mediator complex plays an essential and multi-faceted role in regulation of RNA polymerase II transcription all eukaryotes. Structural analysis yeast has provided understanding the conserved core its interaction with but failed to reveal structure Tail module that contains most subunits targeted by activators repressors. Here we present a molecular model mammalian ( Mus musculus ) Mediator, derived from 4.0 Å resolution cryo-EM map complex. reveals previously unresolved module,...
Despite nearly universal expression of the wild‐type epidermal growth factor receptor (EGFR) and reproducible activity EGFR inhibitors in patients with squamous cell carcinoma head neck (SCCHN), majority will not have objective responses. The mechanisms this intrinsic resistance are well established. We hypothesized that sensitivity to can be predicted based on inhibitors' effects downstream signaling. Cell viability assays were used assess inhibitor gefitinib (ZD1839) 8 SCCHN lines....
Temperature‐responsive magnetic nanomicelles can serve as thermal energy and cargo carriers with controlled drug release functionality. In view of their potential biomedical applications, understanding the modes interaction between nanomaterials living systems evaluation efficiency delivery is utmost importance. this work, we investigate hybrid engineered for platinum complex a biological system at three fundamental levels: subcellular compartments, single cell whole animal. Nanomicelles...
Autism spectrum disorder (ASD) is a group of neurological and developmental disabilities characterised by clinical genetic heterogeneity. The current study aimed to expand ASD genotyping investigating potential associations with SYNE2 mutations. Specifically, the disease-causing variants in 410 trios manifesting neurodevelopmental disorders using whole-exome sequencing were explored. consequences identified studied at transcript level quantitative polymerase chain reaction (qPCR). For...
Triple-negative breast cancer (TNBC) is a highly aggressive subtype characterised by the absence of targetable hormone receptors and increased metastatic rates. As nuclear softening strongly contributes to TNBC's enhanced capacity, increasing stiffness TNBC cells may present promising therapeutic avenue. Previous evidence has demonstrated ability Sirtuin 2 (SIRT2) inhibition induce cytoskeletal reorganisation, key factor in regulating mechanics. Thus, our study aimed investigate effect SIRT2...
Mechanotransduction is defined as the ability of cells to sense mechanical stimuli from their surroundings and translate them into biochemical signals. Epidermal keratinocytes respond cues by altering proliferation, migration, differentiation. In vitro cell culture, however, utilises tissue culture plastic, which significantly stiffer than in vivo environment. Current epidermal models fail consider effects culturing on plastic prior setting up three-dimensional cultures, so impact this...
Epigenetic alterations play a central role in the control of normal and malignant blood cell development. We demonstrate here that expression truncated DNA methyltransferase 3B isoform DNMT3B7, which has been shown to alter cellular epigenetic patterns, decreases overall number hematopoietic stem progenitor cells (HSPCs), markedly diminishes reconstitution within female hormonal microenvironment. Gene profiling HSPCs isolated from DNMT3B7 transgenic embryos identified Apolipoprotein E (Apoe)...
AbstractTreponema pallidum, the agent of syphilis, is a pathogenic spirochete that has no known mechanisms genetic exchange and cannot be continuously cultivated in vitro. A probe based on nucleotide sequence T. pallidum cheA gene was used to screen genomic DNA library. treponemal region containing four open reading frames (orfs) identified. The proteins encoded by these orfs have significant homology with involved bacterial chemotaxis. been designated cheA, cheW, cheX, cheY. cheY genes were...
SYNE2 mutations have been associated with skeletal and cardiac muscle diseases, including Emery-Dreifuss muscular dystrophy (EDMD). Here, we present a 70-year-old male patient pain elevated serum creatine kinase levels in whom whole-exome sequencing revealed novel heterozygous splice site mutation (NM_182914.3:c.15306+2T>G). This is likely to result the loss of donor intron 82. While diagnostic biopsy showed unspecific myopathological findings, immunofluorescence analyses dermal cells...
Eukaryotic cells tether the nucleoskeleton to cytoskeleton via a conserved molecular bridge, called LINC complex. The core of complex comprises SUN-domain and KASH-domain proteins that directly associate within nuclear envelope lumen. Intra- inter-chain disulphide bonds, along with protein interactions, both contribute tertiary quaternary structure vertebrate proteins. significance these bonds role PDIs (protein isomerases) in biology remains unclear. Reducing non-reducing SDS-PAGE analyses...
Treponema pallidum, the agent of syphilis, cannot be continuously cultivated in vitro. To identify treponemal genes encoding exported proteins, we performed TnphoA mutagenesis a T. pallidum genomic DNA library Escherichia coli. Clone 6D2 was chosen for further study based on partial nucleotide sequence obtained from p6D2 containing insertion. A complete open reading frame (orf1) and truncated orf (orf2′) were identified treponemai p6D2. Orf1 encodes hydrophobic protein 531 amino acids with...
AbstractThe recently identified flu operon of Treponema pallidum contains several genes that encode motilityrelated proteins. We have determined the nucleotide sequences three genes, designated flhA, flhF, and or/304, are located immediately downstream flhB gene in fla operon. The flhA encodes a 707-amino acid protein five putative membrane spanning domains. FlhA has strong homology with members family proteins involved flagellar biogenesis regulation/secretion virulence-related flhF...
The Synchrotron X-ray fluorescence micrograph shows the distribution of platinum within a single cancer cell. Pseudocolor images are created by correlating concentrations with color spectrum. In article 1400182, E. A. Rozhkova and co-workers demonstrate efficient delivery cisplatin pro-drug to biological system; this is achieved using hybrid thermosensitive magnetic nanomicelles. For first time, interface between an advanced material cell visualized unprecedented high spatial resolution 30...
The Mediator complex plays an essential and multi-faceted role in regulation of RNA polymerase II transcription all eukaryotes. Structural analysis yeast has provided understanding the conserved core its interaction with but failed to reveal structure Tail module that contains most subunits targeted by activators repressors. Here we present a molecular model mammalian ( Mus musculus ) Mediator, derived from 4.0 Å resolution cryo-EM map complex. reveals previously unresolved module, which...
AbstractWe determined the nucleotide sequence of enolase (eno) gene Treponema pallidum, noncultivable agent syphilis. The deduced amino acid T. pallidum (Eno) is 432 acids long with a predicted molecular mass 46.7 kDa. Eno has high degree homology to sequences prokaryotic and eukaryotic Eno. This first eno reported for bacterium in order Spirochaetales.Key Words: EnolaseglycolysisspirochetesyphilisTreponema