A5011500859- Skin Protection and Aging
- Gene expression and cancer classification
- Estrogen and related hormone effects
- Fibroblast Growth Factor Research
- Molecular Biology Techniques and Applications
- Dermatology and Skin Diseases
- Reproductive System and Pregnancy
- Advanced Biosensing Techniques and Applications
- melanin and skin pigmentation
- Dermatologic Treatments and Research
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Computational Drug Discovery Methods
- Allergic Rhinitis and Sensitization
- Tendon Structure and Treatment
- Cancer-related gene regulation
- Animal testing and alternatives
- interferon and immune responses
- Gut microbiota and health
- Laser Applications in Dentistry and Medicine
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- TGF-β signaling in diseases
- Respiratory viral infections research
- Wound Healing and Treatments
Procter & Gamble (United States)
2013-2024
Oregon Health & Science University
1993
Portland VA Medical Center
1993
University of Nebraska Medical Center
1988-1992
The bone morphogenetic proteins (BMPs) are a group of transforming growth factor β (TGF-β)-related factors whose only receptor identified to date is the product daf-4 gene from Caenorhabditis elegans. Mouse embryonic NIH 3T3 fibroblasts display high-affinity 125I-BMP-4 binding sites. Binding assays not possible with isoform 125I-BMP-2 unless positively charged N-terminal sequence removed create modified BMP-2, 125I-DR-BMP-2. Cross-competition experiments reveal that BMP-2 and BMP-4 interact...
To systematically examine mucosal biopsies for differences in cytokine gene expression and protein secretion.The study included 59 females with irritable bowel syndrome (IBS) 39, otherwise healthy, female volunteers presenting colonoscopy. Colonic from subsets were studied by microarray analysis (IBS, n=9; controls, n=8), quantitative reverse transcription-polymerase chain reaction (qRT-PCR) n=22; n=21), ex vivo biopsy culture n=28, n=10). Biopsies patients active colitis used as...
Human rhinovirus infections cause colds and trigger exacerbations of lower airway diseases.To define changes in gene expression profiles during vivo infections.Nasal epithelial scrapings were obtained before experimental infection, was evaluated by microarray. Naturally acquired infections, cultured human cells, short interfering RNA knockdown used to further evaluate the role viperin infections.Symptom scores viral titers measured subjects inoculated with or sham control, assessed 8 48...
Understanding the changes in skin microbiome and their relationship to host factors during aging remains largely unknown. To better understand this phenomenon, we collected samples for metagenomic factor analyses from forearm, buttock, facial 158 Caucasian females aged 20‒24, 30‒34, 40‒44, 50‒54, 60‒64, 70‒74 years. Metagenomics analysis was performed using 16S ribosomal RNA gene sequencing, whereas sebocyte gland area, lipids, natural moisturizing factors, antimicrobial peptides...
PDGF has been implicated as one of the principal mitogens involved in cutaneous wound healing. While it previously reported that both platelets and monocytes are a source human dermal repair, production by keratinocytes not yet described. In this manuscript, we report cultured keratinocytes. Both A B chain mRNA can be detected cells. only PDGF-AA polypeptide is found significant levels keratinocyte-conditioned culture media, all three isoforms (AA, AB, BB) present detergent-solubilized cell...
Zinc pyrithione (ZPT) is an antimicrobial material with widespread use in antidandruff shampoos and antifouling paints. Despite decades of commercial use, there little understanding its mechanism action. We used a combination genome-wide approaches (yeast deletion mutants microarrays) traditional methods (gene constructs atomic emission) to characterize the activity ZPT against model yeast, Saccharomyces cerevisiae. acts through increase cellular copper levels that leads loss iron-sulfur...
Connectivity mapping is a method used in the pharmaceutical industry to find connections between small molecules, disease states, and genes. The concept can be applied predictive toxicology paradigm chemicals, adverse events, In order assess applicability of technique for purposes, we performed gene array experiments on 34 different chemicals: bisphenol A, genistein, ethinyl-estradiol, tamoxifen, clofibrate, dehydorepiandrosterone, troglitazone, diethylhexyl phthalate, flutamide, trenbolone,...
Even though human sweat is odorless, bacterial growth and decomposition of specific odor precursors in it believed to give rise body humans. While mechanisms generation have been widely studied adults, little known for teenagers pre-pubescent children who distinct composition from immature apocrine sebaceous glands, but are arguably more susceptible the social psychological impact malodor. We integrated information whole microbiome analysis multiple skin sites (underarm, neck, head) time...
The purpose of this study was to determine (1) the transcriptional program elicited by exposure three estrogen receptor (ER) agonists: 17 α-ethynyl estradiol (EE), genistein (Ges), and bisphenol A (BPA) during fetal development rat testis epididymis; (2) whether very low dosages estrogens (evaluated over five orders magnitude dosage) produce unexpected changes in gene expression (i.e., a non-monotonic dose-response curve). In independently conducted experiments, Sprague-Dawley rats were...
Background: To our knowledge, changes in the patterns of whole‐transcriptome gene expression that occur during induction and resolution experimental gingivitis humans were not previously explored using bioinformatic tools. Methods: Gingival biopsy samples collected from 14 subjects a 28‐day stent‐induced model, followed by treatment, at days 28 through 35 analyzed gene‐expression arrays. Biopsy different sites within each subject baseline (day 0), peak 28), 35) processed Gene‐expression data...
Background Dandruff/seborrhoeic dermatitis is a common scalp condition that characterized by flakes, pruritus and sometimes mild erythema. These symptoms reflect tissue level events are poorly understood at the molecular level. Objectives The purpose of this work was: (i) to compare gene expression profiles in subjects with dandruff vs. those without determine key physiological disruptions manifest condition; (ii) effect on profile treatment shampoo containing potentiated zinc pyrithione...
Human rhinovirus and influenza virus infections of the upper airway lead to colds flu can trigger exacerbations lower diseases including asthma chronic obstructive pulmonary disease. Novel diagnostic therapeutic targets are still needed differentiate between cold flu, since clinical course be severe while that is usually more mild. In our investigation infection human respiratory epithelial cells, we used a systems approach identify temporally changing patterns host gene expression from...
We have discovered a soluble form of the plateletderived growth factor (PDGF) alpha receptor, designated SPDGF-Ra, that is produced by and secreted into conditioned medium human osteosarcoma cell line, MG-63.Additionally, sPDGF-Ra activity has been detected in normal blood plasma serum.We achieved partial purification this protein column chromatography using three different affinity matrices: anti-PDGF-Ra monoclonal antibody (mAb) 292.15-Sepharose,PDGF-BB-Sepharose, wheat germ...
Shotgun metagenomics is a powerful platform to characterize human microbiomes. However, translate such survey data into consumer-relevant products or services, it critical have robust workflow. We present tool – spike-in DNA assess performance of workflows. The from two organisms Alivibrio fischeri and Rhodopseudomonas palustris, in ratio 4:1 added samples before extraction. With valid workflow, the output relative abundances these should be close 4. This expectation was tested varying...
Ablative fractional laser treatment is considered the gold standard for skin rejuvenation. In order to understand how works rejuvenate skin, we performed microarray profiling on biopsies identify temporal and dose-response changes in gene expression following treatment. The backs of 14 women were treated with ablative (Fraxel®) 4 mm punch collected from an untreated site at sites 1, 3, 7, 14, 21 28 days after single addition, effect that multiple treatments have rejuvenation, several...