A5016954726- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Veterinary medicine and infectious diseases
- Clostridium difficile and Clostridium perfringens research
- Animal Nutrition and Physiology
- Escherichia coli research studies
- Gut microbiota and health
- Identification and Quantification in Food
- Enterobacteriaceae and Cronobacter Research
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Vitamin D Research Studies
- Antibiotic Use and Resistance
- Zoonotic diseases and public health
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Identification and Susceptibility Testing
- Animal Virus Infections Studies
North Carolina State University
2016-2025
North Central State College
2018-2021
Technical University of Denmark
2020
Ahmadu Bello University
2020
University of Surrey
2020
The Ohio State University
2007
University of Wisconsin–Madison
2007
United States Food and Drug Administration
2006-2007
Center for Veterinary Medicine
2007
Post Graduate Institute of Medical Education and Research
1999
Abstract Objectives The COVID-19 pandemic has had a substantial impact on health systems. WHO Antimicrobial Resistance (AMR) Surveillance and Quality Assessment Collaborating Centres Network conducted survey to assess the effects of AMR surveillance, prevention control. Methods From October December 2020, Global Use System (GLASS) national focal points completed questionnaire, including Likert scales open-ended questions. Data were descriptively analysed, income/regional differences assessed...
Out of the many pathogenic bacterial species that are known, only a fraction readily identifiable directly from complex microbial community using standard next generation DNA sequencing. Long-read sequencing offers potential to identify wider range and differentiate between strains within species, but attaining sufficient accuracy in metagenomes remains challenge.Here, we describe analytically validate LoopSeq, commercially available synthetic long-read (SLR) technology generates highly...
Much uncertainty remains about the origin and public health implications of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA). This study aimed to investigate occurrence prevalence MRSA in general LA-MRSA particular pigs farm workers five states. We collected nasal swabs from at 45 swine herds (21 antibiotic-free herds; 24 conventional herds) Illinois, Iowa, Minnesota, North Carolina Ohio. was isolated 50 1085 (4.6%) 31 148 (20.9%) workers. MRSA-positive people were...
Salmonella enterica is a significant foodborne pathogen, which can be transmitted via several distinct routes, and reports on acquisition of antimicrobial resistance (AMR) are increasing. To better understand the association between human clinical isolates potential environmental/animal reservoirs, whole genome sequencing (WGS) was used to investigate epidemiology AMR patterns within from two adjacent US states.WGS data 200 S. recovered (n = 44), swine 32), poultry 22), farm environment 102)...
ABSTRACT Eleven laboratories collaborated to determine the periodic prevalence of Salmonella in a population dogs and cats United States visiting veterinary clinics. Fecal samples (2,965) solicited from 11 geographically dispersed testing were collected 36 states between January 2012 April 2014 tested using harmonized method. The overall study (3 542) was <1%. (60 2,422) 2.5%. Diarrhea present only 55% positive dogs; however, 3.8% all diarrheic positive, compared with 1.8% nondiarrheic...
Abstract Background Inappropriate use of antimicrobial agents in animal production has led to the development resistance (AMR) foodborne pathogens. Transmission AMR pathogens from reservoirs, particularly chickens human population does occur. Recently, we reported that occupational exposure was a risk factor for multidrug-resistant (MDR) Escherichia coli (E. coli) among poultry-workers. Here determined prevalence and genetic relatedness MDR E. isolated poultry-workers, chickens, poultry...
Background Antimicrobial resistance has emerged as a global health threat. resistant Escherichia coli infections are associated with high morbidity and expenditure when compared caused by susceptible strains. In Nigeria, antimicrobial drugs readily available over-the-counter potential for indiscriminate use poultry farmers eventual development of drug resistance. The objective this study was to investigate prevalence risk factors multi-drug E. among workers (PW) in Abuja, Nigeria. Materials...
Abstract Multidrug-resistant (MDR) Salmonella enterica has been deemed a high-priority pathogen by the World Health Organization. Two hundred and sixty-four isolates recovered over 16-year period (2000 to 2016) from poultry swine production chains, in Brazil, were investigated whole-genome sequencing (WGS). Most international lineages belonging 28 serovars, including, S . serovars Schwarzengrund ST96, Typhimurium ST19, Minnesota ST548, Infantis ST32, Heidelberg ST15, Newport ST45,...
Abstract Background Commensal Escherichia coli residing in the guts of humans and animals are reservoirs multidrug resistance (MDR) genes, including quinolone poultry. This study aimed to characterize quinolones E. recovered from poultry workers, chickens, farm/market environments Abuja, Nigeria. Methods was a cross-sectional conducted between December 2018 April 2019 comprising chickens their environments. characterized isolates stool, faecal environmental samples using antimicrobial...
The objective of this cross-sectional study was to compare the prevalence Salmonella and antimicrobial-resistant Salmonella, as well investigate distribution pathogen in organic conventional broiler poultry farms. Fecal (n = 420), feed 140), drinking water 140) samples were collected from birds at 3 8 weeks age for 2-flock cycles. One house sampled per farm three four farms same company North Carolina. All analyzed presence using selective enrichment techniques. Further phenotypic...
Globally, chicken is known to be a reservoir for the spread of antimicrobial resistance genes humans. In Nigeria, drugs are readily accessible use in poultry production, either preventive or therapeutic purposes. Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) transmissible humans because their zoonotic potentials. People working very closely with chickens on farms markets at greater risk. The aim this study was investigate prevalence and transmission ESBL-EC among...
The Center for Disease Control and Prevention identifies antimicrobial resistant (AMR) Campylobacter as a serious threat to U.S. public health due high community burden, increased transmissibility, limited treatability. National Antimicrobial Resistance Monitoring System (NARMS) plays an important role in surveillance of AMR bacterial pathogens humans, food animals retail meats. This study investigated C . coli jejuni from live animals, poultry carcasses at production, meat North Carolina...
Salmonella serovars are important reservoirs of antimicrobial resistance. Recently, we reported on multidrug-resistant (MDR) enterica serovar Typhimurium strains among pigs with resistance to ampicillin, kanamycin, streptomycin, sulfamethoxazole, and tetracycline (resistance [R] type AKSSuT) amoxicillin-clavulanic acid, chloramphenicol, (R AxACSSuT). In the present study, 67 isolates (39 from humans 28 pigs) clinically Muenchen were characterized. Among porcine isolates, 75% showed seven...
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a major associated with human salmonellosis. A total of 425 clinical S. isolates origin were collected between June 2009 and September 2010 from North Carolina. The further characterized for antimicrobial susceptibility, resistance coding determinants, virulence genes, fingerprint profiles to determine whether they similar or different the strain responsible outbreak due consumption contaminated eggs. Ten phenotypes observed highest...
The aim of this longitudinal study was to determine and compare the prevalences genotypic profiles antimicrobial-resistant (AR) Salmonella isolates from pigs reared in antimicrobial-free (ABF) conventional production systems at farm, slaughter, their environment. We collected 2,889 pig fecal 2,122 environmental (feed, water, soil, lagoon, truck, floor swabs) samples 10 eight ABF cohorts different stages (farrowing, nursery, finishing) slaughter (postevisceration, postchill, mesenteric lymph...
ABSTRACT The aim of this study was to characterize the plasmids carrying antimicrobial resistance (AMR) determinants in multiple Salmonella serotypes recovered from commercial swine farm environment after manure application on land. Manure and soil samples were collected day 0 before six farms North Carolina, sequential recollected days 7, 14, 21 same plots. All environmental processed for , their plasmid contents further characterized. A total 14 isolates including enterica Johannesburg ( n...
Background Escherichia coli is commonly used as an indicator for antimicrobial resistance (AMR) in food, animal, environment, and human surveillance systems. Our study aimed to characterize AMR E . isolated from retail meat purchased grocery stores North Carolina, USA part of the National Antimicrobial Resistance Monitoring System (NARMS). Materials methods Retail chicken (breast, n = 96; giblets, 24), turkey (n 96), pork 96) products were monthly different counties Carolina during 2022....
The resolution of variation within species is critical for interpreting and acting on many microbial measurements. In the key foodborne pathogens
ABSTRACT Wastewater surveillance has emerged as a crucial public health tool for population-level pathogen surveillance. Supported by funding from the American Rescue Plan Act of 2021, FDA‘s genomic epidemiology program, GenomeTrakr, was leveraged to sequence SARS-CoV-2 wastewater sites across United States. This initiative required evaluation, optimization, development, and publication new methods analytical tools spanning sample collection through variant analyses. Version-controlled...
Abstract Campylobacter spp . resistant to fluoroquinolones and macrolides are serious public health threats. Studies aiming identify risk factors for drug-resistant have narrowly focused on antimicrobial use at the farm level. Using chain graphs, we quantified fluoroquinolones- macrolide-resistance in coli isolated from two distinctive swine production systems, conventional antibiotic-free (ABF). The graphs were learned using genotypic phenotypic resistance data 1082 isolates host exposures...
This study delves into how the Bhagavad Gita serves as a guiding framework for sustainable leadership and organizational sustainability through self-transcendence. It emphasizes key concepts in that involves rising above ego-driven desires personal ambitions to embrace higher purpose. The highlights core teachings of Gita, including nishkama karma (selfless action), samatvam (equanimity), dharma (righteous duty), provide valuable ethical practices. research argues leaders who practice focus...