A5018067645- Antimicrobial Resistance in Staphylococcus
- Bacillus and Francisella bacterial research
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- SARS-CoV-2 detection and testing
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Food Safety and Hygiene
- Streptococcal Infections and Treatments
- Microbial Metabolism and Applications
- Advanced biosensing and bioanalysis techniques
- Enterobacteriaceae and Cronobacter Research
- Water-Energy-Food Nexus Studies
- Antimicrobial Peptides and Activities
- Plant and fungal interactions
- Biomedical and Engineering Education
- Botulinum Toxin and Related Neurological Disorders
United States Food and Drug Administration
2013-2024
Center for Food Safety and Applied Nutrition
2014-2024
Mondelēz International (India)
2024
Society for Healthcare Epidemiology of America
2024
University of Maryland, College Park
2018
Food and Drug Administration
2015
Virginia Commonwealth University
2003-2010
Virginia Commonwealth University Medical Center
2002-2004
Nosocomial bloodstream infections (BSIs) are important causes of morbidity and mortality in the United States.Data from a nationwide, concurrent surveillance study (Surveillance Control Pathogens Epidemiological Importance [SCOPE]) were used to examine secular trends epidemiology microbiology nosocomial BSIs.Our detected 24,179 cases BSI 49 US hospitals over 7-year period March 1995 through September 2002 (60 per 10,000 hospital admissions). Eighty-seven percent BSIs monomicrobial....
Background. We identified the predominant pathogens and antimicrobial susceptibilities of nosocomial bloodstream isolates in pediatric patients US Prospective surveillance for infections at 49 hospitals during a 6-year period [Surveillance Control Pathogens Epidemiologic Importance (SCOPE)] detected 22 609 infections, which 3432 occurred ≤16 years age. Results. Gram-positive organisms accounted 65% cases, Gram-negative 24% cases 11% were caused by fungi. The overall crude mortality was 14%...
Bacillus cereus is a group of ubiquitous facultative anaerobic sporeforming Gram-positive rods commonly found in soil. The spores frequently contaminate variety foods, including produce, meat, eggs, and dairy products. Foodborne illnesses associated with toxins produced by B. can result self-limiting diarrhea or vomiting. Plate enumeration methods recommended recognized food authorities to detect the presence potentially contaminated products do not inhibit other competitive bacteria. This...
ABSTRACT Surface waters are considered ecological habitats where Salmonella enterica can persist and disseminate to fresh produce production systems. This study aimed explore the genomic profiles of S. serotypes Typhimurium, Newport, Infantis from surface in Chile, Mexico, Brazil collected between 2019 2022. We analyzed whole genomes 106 S . 161 113 isolates. Our phylogenetic analysis exhibited distinct groupings isolates by their respective countries except for a notable case involving...
ABSTRACT Wastewater surveillance has emerged as a crucial public health tool for population-level pathogen surveillance. Supported by funding from the American Rescue Plan Act of 2021, FDA‘s genomic epidemiology program, GenomeTrakr, was leveraged to sequence SARS-CoV-2 wastewater sites across United States. This initiative required evaluation, optimization, development, and publication new methods analytical tools spanning sample collection through variant analyses. Version-controlled...
The relationship between the composition of SaPI1 transducing particles and those helper phage 80alpha was investigated by direct comparison virion proteins. Twelve proteins were identified sodium dodecyl sulfate-polyacrylamide gel electrophoresis mass spectrometry; all present in both virions, encoded 80alpha. No SaPI1-encoded detected. This confirms prediction that is encapsidated a assembled from phage-encoded
SaPI1 and SaPIbov1 are chromosomal pathogenicity islands in Staphylococcus aureus that carry tst other superantigen genes. They induced to excise replicate by certain phages, efficiently encapsidated SaPI-specific small particles composed of phage virion proteins transferred at very high frequencies. In this study, we have analysed three SaPI genes important for the phage-SaPI interaction, int (integrase) terS (phage terminase subunit homologue) pif interference function). is required...
ABSTRACT Wastewater surveillance has emerged as a crucial public health tool for population-level pathogen surveillance. Supported by funding from the American Rescue Plan Act of 2021, FDA’s genomic epidemiology program, GenomeTrakr, was leveraged to sequence SARS-CoV-2 wastewater sites across United States. This initiative required evaluation, optimization, development, and publication new methods analytical tools spanning sample collection through variant analyses. Version-controlled...
The study objective was to characterize and analyse the distribution of enterotoxins genes encoding in Staphylococcus aureus strains recovered from 601 environment ingredient samples obtained during multiple inspections a bakery implicated two separate staphylococcal food poisoning incidents.Staphylococcus isolates were evaluated using serological assays for identification classical (SEs) SEA-SEE polymerase chain reaction detection newly described SE SE-like enterotoxin seg-seu. Pulsed field...
Every year salmonellosis is responsible for $2.3 billion in costs to the U.S. food industry, with nearly 6% of reported cases associated pork and/or products. Several studies have demonstrated role pigs as Salmonella reservoirs. Furthermore, this pathogen has been identified a potential biological hazard many livestock feeds. The overall objective research was characterize enterica isolates selected swine feed mills by whole-genome sequencing (WGS) and evaluate association season production...
ABSTRACT Staphylococcal contamination of food products and staphylococcal food-borne illnesses continue to be a problem worldwide. Screening for the presence Staphylococcus aureus and/or enterotoxins using traditional methods is laborious. Reliable rapid multiplex detection from single extract or culture supernatant would simplify testing. A fluorescence-based cytometric bead array was developed enterotoxin B (SEB), magnetic microspheres coupled with either an engineered,...
The aim of this study was to evaluate the performance immunodetection methods and PCR analysis enterotoxigenic Bacillus cereus strains.Eighty-eight B. group strains linked food-borne outbreaks illnesses were studied with 30 exclusivity nonenterotoxigenic including amyoliquifaciens, subtilis, Staphylococcus aureus, Salmonella Escherichia coli for assessment. results showed 80% agreement immunoassays Nhe target 84% Hbl product. All serologically negative.PCR has proven be a valuable tool when...
Staphylococcus aureus continues to play a significant role in foodborne outbreak investigations, with numerous individuals sickened each year after ingesting assorted foods contaminated staphylococcal enterotoxins. The purpose of this study was evaluate the use several methods for screening, detection, and enterotoxin serotyping bacterial strains classical enterotoxins (SEs; SEA, SEB, SEC, SED, SEE) newly described SE SE-like genes (seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, ser,...
Biofilms are a frequent cause of food contamination potentially pathogenic bacteria, such as Staphylococcus aureus . Given its vast role in human disease, the possible impact biofilm-producing S. isolates processing environment is evident. Sixty-nine collected from one firm following multiple staphylococcal poisoning outbreak investigations were utilized for this analysis. Strain evaluations performed to establish virulence determinants and evolutionary relationships using data generated by...
Culture-independent metagenomic sequencing of enriched agricultural water could expedite the detection and virulotyping Shiga toxin-producing Escherichia coli (STEC). We previously determined limits a complete, closed metagenome-assembled genome (MAG) assembly fragmented MAG for O157:H7 in using long reads (Oxford Nanopore Technologies, Oxford), which were 107 105 CFU/ml, respectively. However, nanopore assemblies did not have enough accuracy to be used Single Nucleotide Polymorphism (SNP)...
Context: The genomic surveillance of foodborne pathogens in the United States has grown exponentially past decade, grounded a powerful combination novel sequencing technologies, bioinformatic approaches, data-sharing networks, and metadata harmonization efforts. This practice report examines recent advances epidemiology as applied to food safety programs delineates State, Tribal, Local, Territorial infrastructure necessary for continued life-saving improvements public health. Program:...
Abstract Over 100 individuals were sickened after ingesting an assortment of desserts linked to four staphylococcal food poisoning outbreaks leading investigators products manufactured by I llinois bakery. The investigative team identified substantial deviations from the current G ood M anufacturing P ractice R egulations, 21 CFR Part 110, during multiple visits A total 299 environmental swabs and 16 raw ingredients collected for bacteriological analysis. S taphylococcus aureus isolates...