A5071051135- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Opioid Use Disorder Treatment
- Mycobacterium research and diagnosis
- Pain Management and Opioid Use
- Heavy Metal Exposure and Toxicity
- Poisoning and overdose treatments
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- Forensic Toxicology and Drug Analysis
- Chemical Safety and Risk Management
- Climate Change and Health Impacts
- Viral gastroenteritis research and epidemiology
- Suicide and Self-Harm Studies
- Public Health Policies and Education
- Risk Perception and Management
- Digital Rights Management and Security
- Food Safety and Hygiene
- Health, Environment, Cognitive Aging
- Bacteriophages and microbial interactions
Association of Public Health Laboratories
2024
Mondelēz International (India)
2024
Society for Healthcare Epidemiology of America
2024
Rhode Island Department of Health
2013-2023
Providence College
2013-2023
King's College London
2023
University of Michigan
2021
Rogers (United States)
2021
Brown University
2021
Johns Hopkins University
2021
The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear need for high-throughput, multiplexed and sensitive assays detecting severe acute respiratory syndrome 2 (SARS-CoV-2) other viruses their emerging variants. Here, we present cost-effective virus variant detection platform, called microfluidic Combinatorial Arrayed Reactions Multiplexed Evaluation of Nucleic acids (mCARMEN), which combines CRISPR-based diagnostics microfluidics with streamlined workflow clinical use. We...
The SARS-CoV-2 Delta variant rose to dominance in mid-2021, likely propelled by an estimated 40%–80% increased transmissibility over Alpha. To investigate if this ostensible difference is uniform across populations, we partner with public health programs from all six states New England the United States. We compare logistic growth rates during each variant's respective emergence period, finding that emerged 1.37–2.63 times faster than Alpha (range states). compute variant-specific effective...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant quickly rose to dominance in mid-2021, displacing other variants, including Alpha. Studies using data from the United Kingdom and India estimated that was 40-80% more transmissible than Alpha, allowing become globally dominant variant. However, it unclear if ostensible difference relative transmissibility due mostly innate properties of Delta’s infectiousness or differences study populations. To...
Abstract Serology testing of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is increasingly being used during the current pandemic disease 2019 (COVID‐19), although its clinical and epidemiologic utilities are still debatable. Characterizing these assays provides scientific basis to best use them. The study assessed one chemiluminescent assay (Abbott COVID‐2 IgG) two lateral flow (STANDARD Q [SQ] IgM/IgG Duo Wondfo total antibody test) using 113 blood samples from 71...
Objectives. To characterize statewide seroprevalence and point prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Rhode Island. Methods. We conducted a cross-sectional survey randomly selected households across Island May 2020. Antibody-based polymerase chain reaction (PCR)–based tests for SARS-CoV-2 were offered. Hispanics/Latinos African Americans/Blacks oversampled to ensure adequate representation. Seroprevalence estimations accounted test sensitivity...
The absence of a comprehensive national playbook for developing and deploying testing has hindered the United States' ability to rapidly suppress recent biological emergencies (for example, COVID-19 pandemic outbreaks mpox). We describe here Testing Playbook Biological Emergencies, we developed. It includes set decisions actions US officials take at specific times during infectious disease implement ensure that available meets clinical public health needs. Although States had multiple plans...
Abstract Serology testing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is increasingly being used during the current pandemic Coronavirus Disease 2019 (COVID-19). The clinical and epidemiologic utilities antibody-based SARS-CoV-2 are under debate. Characterizing these assays helps to understand disease provides scientific basis for deciding how best use assays. study assessed one chemiluminescent assay (Abbott COVID-2 IgG) two lateral flow (STANDARD Q [SQ] IgM/IgG Duo...
Human T-lymphotropic virus type 1 (HTLV-1) infection has been discovered recently in people of Amerindian descent living coastal areas British Columbia, Canada. DNA sequencing combined with phylogenetic analysis and restriction fragment length polymorphism (RFLP) typing HTLV-1 strains recovered from these Columbia Indians (BCI) was conducted. Sequence-based trees distributed the BCI isolates among Japanese subcluster (subcluster B) geographically widely A) large cosmopolitan cluster. Long...
Context: The genomic surveillance of foodborne pathogens in the United States has grown exponentially past decade, grounded a powerful combination novel sequencing technologies, bioinformatic approaches, data-sharing networks, and metadata harmonization efforts. This practice report examines recent advances epidemiology as applied to food safety programs delineates State, Tribal, Local, Territorial infrastructure necessary for continued life-saving improvements public health. Program:...
Isolates of HTLV-I have been characterized from a number different regions the world; however, there has not nucleotide sequence analysis an isolate South American country. Reported here is individual Chile identified with HTLV-I-associated neurological disease HAM/TSP. The sera and nucleic acid present in peripheral blood lymphocytes this Chilean HAM/TSP patient over two year period are characterized. During time, patient's condition grew progressively worse. While serological profile was...
The entire envelope gene of human T cell leukaemia virus type I (HTLV-I) has been successfully expressed in a baculovirus non-fusion vector system. HTLV-I protein accumulated within the insect cells as inclusion bodies which allowed efficient recovery recombinant protein. In an attempt to study role glycoprotein immunogenic target, mice were immunized with (env-I.B.) presence or absence adjuvant. Antibodies broad specificity produced against adjuvant detected by Western blotting,...
Abstract Background: Epidemic projections and public health policies addressing Coronavirus disease (COVID)-19 have been implemented without data reporting on the seroconversion of population since scalable antibody testing has only recently become available. Methods: We measured percentage severe acute respiratory syndrome- Coronavirus-2 (SARS-CoV-2) seropositive individuals from 2,008 blood donors drawn in state Rhode Island (RI). utilized multiple platforms, including lateral flow...
Heterologous expression of the human T-cell lymphotropic virus type 1 (HTLV-1) envelope surface glycoprotein (gp46) in a vaccinia virus/T7 polymerase system resulted production authentic recombinant gp46. Five differentially glycosylated forms protein were produced by this mammalian system, as demonstrated tunicamycin inhibition N-glycosylation and N-glycan removal with endoglycosidase H glycopeptidase F. These studies revealed that all four potential sites gp46 used for oligosaccharide...
Epidemic projections and public health policies addressing Coronavirus disease (COVID)-19 have been implemented without data reporting on the seroconversion of population since scalable antibody testing has only recently become available. We measured percentage severe acute respiratory syndrome- Coronavirus-2 (SARS-CoV-2) seropositive individuals from 2008 blood donors drawn in state Rhode Island (RI). utilized multiple platforms, including lateral flow immunoassays (LFAs), enzyme-linked...
Abstract Epidemic projections and public health policies addressing Coronavirus disease (COVID)-19 have been implemented without data reporting on the seroconversion of population since scalable antibody testing has only recently become available. We measured percentage severe acute respiratory syndrome-Coronavirus-2 (SARS-CoV-2) seropositive individuals from 2,008 blood donors drawn in state Rhode Island (RI). utilized multiple platforms, including lateral flow immunoassays (LFAs),...
Next-generation sequencing (NGS) is the primary method used to monitor distribution and emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants around world; however, it costly time-consuming perform not widely available in low-resourced geographical regions. Pyrosequencing has potential augment surveillance efforts by providing information on specific targeted mutations for rapid identification circulating emerging variants. The current study describes development...
Opioids biosurveillance is a new approach to public health surveillance of non-fatal overdoses that relies upon laboratory analysis residual biospecimens from hospitals treating opioids overdoses. In Rhode Island (RI), USA, report suspected opioid the Department Health. Residual specimens associated with these are submitted State Health Laboratories for further characterization. This project aimed characterize through toxicological testing urine during initial 2-year period implementation in...
Objective. The need for public health laboratories (PHLs) to prioritize resources has led increased interest in sharing diagnostic services. To address this concept tuberculosis (TB) testing, the New York State Department of Health Wadsworth Center and Rhode Island Laboratories assessed feasibility shared services detection characterization Mycobacterium complex (MTBC). Methods. We multiple aspects including shipping, reporting, cost. shipped MTBC-positive specimens isolates Center. Average...