Glen R. Gallagher
A5006175160- COVID-19 epidemiological studies
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Diabetes and associated disorders
- COVID-19 and healthcare impacts
- SARS-CoV-2 detection and testing
- Infection Control and Ventilation
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Pancreatic function and diabetes
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- melanin and skin pigmentation
- Virology and Viral Diseases
- RNA Research and Splicing
- Travel-related health issues
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Forensic Toxicology and Drug Analysis
- Opioid Use Disorder Treatment
Rhode Island Department of Health
2022-2024
Providence College
2022-2024
Massachusetts Department of Public Health
2020-2023
Florida Department of Health
2012-2021
Miami Dade College
2021
Allegheny County
2021
Centers for Disease Control and Prevention
2012-2021
Georgia Department of Public Health
2021
Pennsylvania Department of Health
2021
University of Massachusetts Chan Medical School
2013-2017
During July 2021, 469 cases of COVID-19 associated with multiple summer events and large public gatherings in a town Barnstable County, Massachusetts, were identified among Massachusetts residents; vaccination coverage eligible residents was 69%. Approximately three quarters (346; 74%) occurred fully vaccinated persons (those who had completed 2-dose course mRNA vaccine [Pfizer-BioNTech or Moderna] received single dose Janssen [Johnson & Johnson] ≥14 days before exposure). Genomic sequencing...
Phylogenetics of superspreading One important characteristic coronavirus epidemiology is the occurrence events. These are marked by a disproportionate number cases originating from often-times asymptomatic individuals. Using rich sequence dataset early stages Boston outbreak, Lemieux et al. identified events in specific settings and analyzed them phylogenetically (see Perspective Alizon). ancestral trait inference, authors several importation events, further investigated context contribution...
On May 17, 2022, the Massachusetts Department of Public Health (MDPH) Laboratory Response Network (LRN) laboratory confirmed presence orthopoxvirus DNA via real-time polymerase chain reaction (PCR) from lesion swabs obtained a resident. Orthopoxviruses include Monkeypox virus, causative agent monkeypox. Subsequent PCR testing at CDC on 18 that patient was infected with West African clade virus. Since then, cases* have been reported by nine states. In addition, 28 countries and territories,†...
Monkeypox is a viral zoonotic disease endemic in Central and West Africa. In May 2022, dozens of non-endemic countries reported hundreds monkeypox cases, most with no epidemiological link to We identified two lineages virus (MPXV) among 2021 seven 2022 US cases: the major outbreak variant called B.1 minor contemporaneously sampled A.2. Analyses mutations these variants revealed an extreme preference for GA-to-AA indicative human APOBEC3 cytosine deaminase activity Clade IIb MPXV (previously...
The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear need for high-throughput, multiplexed and sensitive assays detecting severe acute respiratory syndrome 2 (SARS-CoV-2) other viruses their emerging variants. Here, we present cost-effective virus variant detection platform, called microfluidic Combinatorial Arrayed Reactions Multiplexed Evaluation of Nucleic acids (mCARMEN), which combines CRISPR-based diagnostics microfluidics with streamlined workflow clinical use. We...
The SARS-CoV-2 Delta variant rose to dominance in mid-2021, likely propelled by an estimated 40%–80% increased transmissibility over Alpha. To investigate if this ostensible difference is uniform across populations, we partner with public health programs from all six states New England the United States. We compare logistic growth rates during each variant's respective emergence period, finding that emerged 1.37–2.63 times faster than Alpha (range states). compute variant-specific effective...
Little is known about clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in care hospitals.
Abstract After 3 dengue cases were acquired in Key West, Florida, we conducted a serosurvey to determine the scope of outbreak. Thirteen residents showed recent infection (infection rate 5%; 90% CI 2%–8%), demonstrating reemergence Florida. Increased awareness among health care providers is needed.
We describe 3 instances of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission despite medical masks and eye protection, including the source person being masked, exposed both parties masked. Whole genome sequencing confirmed perfect homology between persons' viruses in all cases.
The 2022 multicountry mpox outbreak concurrent with the ongoing Coronavirus Disease 2019 (COVID-19) pandemic further highlighted need for genomic surveillance and rapid pathogen whole-genome sequencing. While metagenomic sequencing approaches have been used to sequence many of early infections, these methods are resource intensive require samples high viral DNA concentrations. Given atypical clinical presentation cases associated uncertainty regarding load across both course infection...
Influenza A virus (IAV) lacks the enzyme for adding 5' caps to its RNAs and snatches ends of host capped prime transcription. Neither preference RNA sequences snatched nor effect cap-snatching on processes is completely defined. Previous studies influenza used poly(A)-selected from infected cells or relied annotated genes define RNAs, thus lack details many noncoding including snRNAs, snoRNAs, promoter-associated small (cs)RNAs, which are made by "paused" Pol II during transcription...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant quickly rose to dominance in mid-2021, displacing other variants, including Alpha. Studies using data from the United Kingdom and India estimated that was 40-80% more transmissible than Alpha, allowing become globally dominant variant. However, it unclear if ostensible difference relative transmissibility due mostly innate properties of Delta’s infectiousness or differences study populations. To...
An outbreak of over 1,000 COVID-19 cases in Provincetown, Massachusetts (MA), July 2021-the first large mostly vaccinated individuals the US-prompted a comprehensive public health response, motivating changes to national masking recommendations and raising questions about infection transmission among individuals. To address these questions, we combined viral genomic epidemiological data from 467 individuals, including 40% outbreak-associated cases. The Delta variant accounted for 99% this...
Abstract Monkeypox is a viral zoonotic disease endemic in Central and West Africa. In May 2022, dozens of non-endemic countries reported hundreds monkeypox cases, most with no epidemiological link to We identified two lineages virus (MPXV) among nine 2021 2022 U.S. cases. A case was highly similar the MPXV outbreak variant, suggesting common ancestor. Analysis mutations these revealed an extreme preference for GA-to-AA indicative APOBEC3 cytosine deaminase activity that shared African since...
Abstract SARS-CoV-2 has caused a severe, ongoing outbreak of COVID-19 in Massachusetts with 111,070 confirmed cases and 8,433 deaths as August 1, 2020. To investigate the introduction, spread, epidemiology Boston area, we sequenced analyzed 772 complete genomes from region, including nearly all within first week epidemic hundreds major outbreaks at conference, nursing facility, among homeless shelter guests staff. The data reveal over 80 introductions into predominantly elsewhere United...
This study examines substances identified during testing of counterfeit prescription pills seized by law enforcement in Rhode Island from 2017 to 2022.
ABSTRACT Influenza A virus (IAV) is a major cause of morbidity and mortality throughout the world. Current antiviral therapies include oseltamivir, neuraminidase inhibitor that prevents release nascent viral particles from infected cells. However, IAV genome can evolve rapidly, oseltamivir resistance mutations have been detected in numerous clinical samples. Using an vitro evolution platform whole-genome population sequencing, we investigated genomics during development resistance. Strain...
The regulation of gene expression is accomplished by both genetic and epigenetic means required for the precise control development neural crest. In hdac1b382 mutants, craniofacial cartilage defective in two distinct ways. First, fewer hoxb3a, dlx2 dlx3-expressing posterior branchial arch precursors are specified many those that consequently undergo apoptosis. Second, contrast, normal numbers progenitors present anterior mandibular hyoid arches, but chondrocyte fail to terminally...
Type 1 diabetes (T1D) is characterized by the destruction of insulin-producing β-cells pancreatic islets. Genetic and environmental factors both contribute to T1D development. Viral infection with enteroviruses a suspected trigger for T1D, but causal role remains unproven controversial. Studies in animals are problematic because species-specific differences host cell susceptibility immune responses candidate viral pathogens such as coxsackievirus B (CVB). In order resolve controversial...
Genomic surveillance of monkeypox virus (MPXV) during the 2022 outbreak has been mainly focused on single nucleotide polymorphism (SNP) changes. DNA viruses, including MPXV, have a lower SNP mutation rate than RNA viruses due to higher fidelity replication machinery. We identified large genomic rearrangement in MPXV sequence from case state Minnesota (MN), USA, an abnormal, uneven read mapping coverage profile whole-genome sequencing (WGS) data. further screened WGS data 206 U.S. samples and...
Enteroviral infections have been associated with the development of type 1 diabetes (T1D), a chronic inflammatory disease characterized by autoimmune destruction insulin-producing pancreatic beta cells. Cultured human islets, including cells, can be infected coxsackievirus B4 (CVB4) and thus are useful for understanding cellular responses to infection. We performed quantitative mass spectrometry analysis on cultured primary islets CVB4 identify molecules pathways altered upon Corresponding...
Abstract The 2022 multi-country monkeypox (mpox) outbreak concurrent with the ongoing COVID-19 pandemic has further highlighted need for genomic surveillance and rapid pathogen whole genome sequencing. While metagenomic sequencing approaches have been used to sequence many of early mpox infections, these methods are resource intensive require samples high viral DNA concentrations. Given atypical clinical presentation cases associated uncertainty regarding load across both course infection...