A5088135588- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Toxin Mechanisms and Immunotoxins
- Virus-based gene therapy research
- Antimicrobial Resistance in Staphylococcus
- Immune Response and Inflammation
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- SARS-CoV-2 and COVID-19 Research
- Nanowire Synthesis and Applications
- Receptor Mechanisms and Signaling
- Lymphoma Diagnosis and Treatment
- Synthesis and Biological Evaluation
- Immunodeficiency and Autoimmune Disorders
- Sarcoma Diagnosis and Treatment
- Cancer Cells and Metastasis
University of Illinois Urbana-Champaign
2015-2024
University of Illinois System
2003-2022
Urbana University
1996-2020
University of Michigan–Ann Arbor
2004-2016
Jackson Foundation
2016
Stanford University
2006-2016
Montreal Neurological Institute and Hospital
2016
Guthy-Jackson Charitable Foundation
2016
University of California, San Francisco
2016
Harbor–UCLA Medical Center
2016
Despite lack of tumor control in many models, spontaneous T cell priming occurs frequently response to a growing tumor. However, the innate immune mechanisms that promote natural antitumor responses are undefined. In human metastatic melanoma, there was correlation between type I interferon (IFN) transcriptional profile and markers tissue. mice, IFN-β produced by CD11c+ cells after implantation, tumor-induced defective mice lacking IFN-α/βR or Stat1. IFN signaling required hematopoietic...
A decoy receptor for SARS-CoV-2 For severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to enter human cells, the spike protein on surface of virus must bind host protein, angiotensin-converting enzyme (ACE2). soluble version is being explored as a therapeutic. Chan et al. used deep mutagenesis identify ACE2 mutants that more tightly and combined mutations further increase binding affinity (see Perspective by DeKosky). promising variant was engineered be stable dimer has protein; it...
Targeting cancer cells, as well the nonmalignant stromal cells cross-presenting tumor antigen (Ag), can lead to complete destruction of well-established solid tumors by adoptively transferred Ag-specific cytotoxic T lymphocytes (CTLs). If, however, express only low levels Ag, then are not destroyed, and escapes Ag loss variants. We show that treating expressing with local irradiation or a chemotherapeutic drug causes sufficient release sensitize for CTLs. This was shown directly using high...
T cell receptors (TCRs) exhibit genetic and structural diversity similar to antibodies, but they have binding affinities that are several orders of magnitude lower. It has been suggested TCRs undergo selection in vivo maintain lower affinities. Here, we show there is not an inherent or limitation on higher affinity. Higher-affinity TCR variants were generated the absence selective pressures by using yeast display from a library Vα CDR3 mutants. Selected mutants had greater than 100-fold...
Clones of cytotoxic T lymphocytes (CTL) differ in their specific reactivity with diverse target cell antigens. To learn about the uniqueness individual CTL clones we injected rats and mice cloned an effort to prepare clone-specific antisera analyze surface molecules that were immunoprecipitated by these antisera. Three studied. They all derived from BALB.B for antigens encoded major histocompatibility complex H-2d haplotype. Antisera raised against contained antibodies lymphocyte...
The molecular basis for the dependence of antigen recognition by T cells on products major histocompatibility complex (MHC) is unknown, and antigenic structures that are actually bound T-cell receptors ill-defined. In this study, we asked whether a monoclonal antibody (mAb) reacts with receptor clone murine cytotoxic lymphocytes (CTL) not other CTL clones can substitute clone's natural ligand in specific cytolytic reactions. To answer question, mAb (1B2) to (2C) was attached covalently...
Abstract Adoptive T cell therapies have achieved significant clinical responses, especially in hematopoietic cancers. Two types of receptor systems been used to redirect the activity cells, normal heterodimeric TCRs or synthetic chimeric Ag receptors (CARs). recognize peptide-HLA complexes whereas CARs typically use an Ab-derived single-chain fragments variable that recognizes cancer-associated cell-surface Ags. Although both mediate diverse effector functions, a quantitative comparison...
The heterodimeric αβ T cell receptor (TCR) for antigen is the key determinant of specificity. structure TCR very similar to that antibodies, but engineering TCRs by directed evolution with combinatorial display libraries has not been accomplished date. Here, we report yeast surface a was achieved only after mutation specific variable region residues. These residues are located in two regions TCR, at interface α- and β-chains β-chain framework thought be proximity CD3 signal-transduction...