A5088099999- Global Public Health Policies and Epidemiology
- Chronic Disease Management Strategies
- Clostridium difficile and Clostridium perfringens research
- Gestational Diabetes Research and Management
- Obesity, Physical Activity, Diet
- Healthcare Systems and Reforms
- Gut microbiota and health
- Mobile Health and mHealth Applications
- Diabetes Management and Education
- Cardiovascular Health and Risk Factors
- Blood Pressure and Hypertension Studies
- Global Cancer Incidence and Screening
- Barrier Structure and Function Studies
- Nutritional Studies and Diet
- Pregnancy and preeclampsia studies
- Child Nutrition and Water Access
- Global Maternal and Child Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Health disparities and outcomes
- Virus-based gene therapy research
- Hemoglobinopathies and Related Disorders
- CRISPR and Genetic Engineering
- Climate variability and models
- Birth, Development, and Health
- Cervical Cancer and HPV Research
Yale University
2020-2025
Kathmandu University
2017-2025
Dhulikhel Hospital
2018-2025
University of Pittsburgh
2014-2025
Patan Academy of Health Sciences
2025
B.P. Koirala Institute of Health Sciences
2024
Government of Nepal
2023
Tribhuvan University
2019-2023
Institute for Social and Environmental Research-Nepal
2022
Rutgers, The State University of New Jersey
2022
Clostridium perfringens is an extremely versatile pathogen of humans and livestock, causing wound infections like gas gangrene (clostridial myonecrosis), enteritis/enterocolitis (including one the most common human food-borne illnesses), enterotoxemia (where toxins produced in intestine are absorbed damage distant organs such as brain). The virulence this Gram-positive, spore-forming, anaerobe largely attributable to its copious toxin production; diverse actions roles infection these now...
Childhood overweight/obesity has become a major public health concern globally because of its adverse consequences and escalating prevalence. The factors underlying the disease conditions manifested during adulthood commonly originate in childhood. Nepal is going through transition where under-nutrition co-exists with obesity; however, there lack well-documented information on childhood overweight or obesity Nepal. This study was carried out to determine prevalence associated among urban...
Significance Sickle cell anemia (SCA) is a common monogenic disorder associated with significant morbidity and mortality high incidence of unexplained sudden death in young adults. With the prevention infections, there an increasing appreciation for cardiopulmonary complications cardiac phenotype that cannot be solely attributed to chronic anemia. We used mouse models SCA iron-deficient show distinct functional, pathological, ultrastructural, molecular features causing unique restrictive...
Although previous studies have suggested alarming rise in the prevalence of hypertension Nepal, there is dearth information on its awareness, treatment and control. In this cross-sectional study, we assessed control among 298 hypertensive adults from suburban town Dhulikhel, Nepal.This study based Dhulikhel Heart Study, which included 1073 adults, aged ≥18 years, recruited randomly selected households. Comprehensive health interviews blood pressure measurements were completed during home...
Abstract Background Patient satisfaction is one proxy indicator of the health care quality; however, enhancing patient in low-income settings very challenging due to inadequacy resources as well low literacy among patients. In this study, we assess and its correlates a tertiary public hospital Nepal. Methods We conducted cross sectional study at outpatient department Bhaktapur Hospital To recruit participants for applied systematic random sampling method. Our used validated Satisfaction...
Clostridium perfringens enterotoxin (CPE) contributes to several important human gastrointestinal (GI) diseases. This toxin and its derivatives are also being explored for translational applications, i.e., cancer therapy or drug delivery. Some, but not all, members of the 24-member claudin (Cldn) family mammalian tight junction proteins can serve as CPE receptors. Among Cldns (hCldns), hCldn-3 -4 known convey sensitivity when expressed by fibroblast transfectants. However, other reportedly...
ABSTRACT In humans and livestock, Clostridium perfringens is an important cause of intestinal infections that manifest as enteritis, enterocolitis, or enterotoxemia. This virulence largely related to the toxin-producing ability C. . article primarily focuses on type F strains a very common human food poisoning many cases nonfoodborne gastrointestinal diseases. The enteric dependent their produce enterotoxin (CPE). CPE has unique amino acid sequence but belongs structurally aerolysin...
Background Inappropriate use of antimicrobials is a global public health issue, particularly in developing countries, including Nepal, where over-the-counter sales and self-medication are common. Objectives To explore knowledge, perceptions, practices their resistance among medicine dispensers community members Nepal. Methods The study was conducted three rural five urban municipalities the Kavrepalanchok district from May 2021 to August 2021. Data were collected using two qualitative...
C. perfringens type F isolates are a leading cause of food poisoning and antibiotic-associated diarrhea. Type isolate virulence requires production enterotoxin [CPE], which acts by forming large pore complexes in host cell plasma membranes. During disease, CPE is produced the intestines when strains undergo sporulation. The toxin then released into intestinal lumen mother lyses at completion Once present lumen, encounters proteases. This study examined vitro , ex vivo processing proteases...
The migration of health experts seeking better opportunities, both nationally and internationally, is a growing concern due to its impact on systems, particularly in developing countries. provision cardiovascular other specialized medical care requires skilled workforce, yet the factors driving brain drain among cardiac workers remain underexplored. This study assessed influencing healthcare from perspective policymakers Nepal. We conducted cross-sectional qualitative 32 key informants...
The risk of hypertension varies based on ethnicity, environmental factors, and genetic predispositions. Studies have reported a higher cardiovascular diseases (CVD) among the Newar ethnic groups in Nepal. However, analysis for Methylenetetrahydrofolate reductase (MTHFR C677T) gene mutations, serum homocysteine, high-sensitivity C-reactive protein (hs-CRP) levels across different ethnicities remains unexplored. Sociodemographic information baseline data 489 participants were obtained from...
C. perfringens type F isolates are a leading cause of food poisoning and antibiotic-associated diarrhea. Type isolate virulence requires production enterotoxin [CPE], which acts by forming large pore complexes in host cell plasma membranes. During GI disease, CPE is produced the intestines when strains undergo sporulation. The toxin then released into intestinal lumen mother lyses at completion Once present lumen, encounters proteases. This study examined vitro, ex vivo, vivo processing...
ABSTRACT Clostridium perfringens enterotoxin (CPE) is responsible for causing the gastrointestinal symptoms of C. type A food poisoning, second most commonly identified bacterial food-borne illness in United States. CPE produced by sporulating cells small intestinal lumen, where it then causes epithelial cell damage and villous blunting that leads to diarrhea cramping. Those effects are typically self-limiting; however, severe outbreaks this particularly two occurring psychiatric...