A5019417967- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Pancreatic function and diabetes
- Bacterial Genetics and Biotechnology
- Antifungal resistance and susceptibility
- Diet, Metabolism, and Disease
- Advanced biosensing and bioanalysis techniques
- Natural Antidiabetic Agents Studies
- Health and Lifestyle Studies
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Biochemical and Structural Characterization
- Metabolism, Diabetes, and Cancer
- Fungal Infections and Studies
- Liver Disease Diagnosis and Treatment
- Regulation of Appetite and Obesity
- Cystic Fibrosis Research Advances
- SARS-CoV-2 detection and testing
- Anodic Oxide Films and Nanostructures
- DNA Repair Mechanisms
- Diabetes and associated disorders
- RNA and protein synthesis mechanisms
- Titanium Alloys Microstructure and Properties
- Adipose Tissue and Metabolism
Leitat Technological Center
2018-2024
Instituto de Investigación Sanitaria La Fe
2016-2024
Hospital Universitari i Politècnic La Fe
2020-2024
Universidad de Extremadura
2003-2022
Universidad Cardenal Herrera CEU
2002-2021
Instituto Valenciano de Investigaciones Agrarias
2007-2019
Instituto de Biomedicina de Valencia
2013
Agrobiotechnology Institute
2013
Universitat Autònoma de Barcelona
2013
Instituto de Agroquímica y Tecnología de Alimentos
2013
ABSTRACT Staphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability bacteria to produce biofilm. Here, we report relationship between chronic bovine mastitis and biofilm formation. We have classified S. isolates into three groups based on presence particular genetic elements required for formation: group 1 ( ica + bap ), 2 , negative), 3 negative, negative). Overall, animals naturally infected had lower milk somatic cell...
The biofilm-associated protein (Bap) is a surface implicated in biofilm formation by Staphylococcus aureus isolated from chronic mastitis infections. bap gene carried putative composite transposon inserted SaPIbov2, mobile staphylococcal pathogenicity island. In this study, orthologue genes several species, including epidermidis , chromogenes xylosus simulans and hyicus were identified, cloned sequenced. Sequence analysis comparison of the these species revealed very high sequence...
Phenotypic biotyping has traditionally been used to differentiate bacteria occupying distinct ecological niches such as host species. For example, the capacity of Staphylococcus aureus from sheep coagulate ruminant plasma, reported over 60 years ago, led description small and bovine S. ecovars. The great majority isolates are represented by a single, widespread clonal complex (CC133) aureus, but its evolutionary origin molecular basis for tropism remain unknown. Here, we provide evidence...
ABSTRACT Staphylococcus epidermidis biofilm formation is associated with the production of polysaccharide intercellular adhesin (PIA)--poly- N -acetylglucosamine (PNAG) by products icaADBC operon. Recent evidence indicates that SarA, a central regulatory element controls aureus virulence factors, essential for synthesis PIA/PNAG and ensuing development in this species. Based on presence sarA homolog, we hypothesized SarA could also be involved regulation process S. . To investigate this,...
Summary Staphylococci adapt specifically to various animal hosts by genetically determined mechanisms that are not well understood. One such adaptation involves the ability coagulate host plasma, which strains isolated from ruminants or horses can be differentiated closely related human strains. Here, we report first this differential coagulation activity is due animal‐specific alleles of von Willebrand factor‐binding protein (vWbp) gene, vwb , and second these carried highly mobile...
ABSTRACT The adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components the host is one key steps in pathogenesis staphylococcal infections. S. presents a family adhesins called MSCRAMMs (microbial surface recognizing adhesive matrix molecules) that specifically recognize components. We examined influence biofilm-associated protein (Bap) expression on proteins, epithelial cell cultures, mammary gland sections colonization an vivo infection model. Bap-positive...
SaPIbov2 is a member of the SaPI family staphylococcal pathogenicity islands and very closely related to SaPIbov1. Typically, certain temperate phages can induce excision replication one or more these package them into special small phage-like particles commensurate with their genome sizes (referred as excision-replication-packaging [ERP] cycle). We have studied phage-SaPI interaction in some depth using SaPIbov2, reference role its integrase. demonstrate here that be induced replicate by...
Candida auris is an emergent multidrug-resistant fungal pathogen considered a severe global threat due to its capacity cause nosocomial outbreaks and deep-seated infections with high transmissibility mortality. However, evidence on pathogenicity the complex host-pathogen interactions still limited. This study used in vivo invertebrate model Galleria mellonella assess virulence, exploring mortality kinetics, melanization response, morphological changes after infection compared albicans...
We have previously shown that the glucose intolerance and hyperglycemic state in GK rat, a new spontaneous model of non-insulin-dependent (type II) diabetes without obesity, are partly accounted for by an alteration pancreatic B cell response. On other hand, hyperglycemic-hyperinsulinemic pattern these rats suggests decrease response to insulin basal state. In present study, vivo action was assessed 8-wk-old females at submaximal (euglycemic clamp) levels. Overall utilization (OGU),...
The SaPIs are chromosomal islands in staphylococci and other Gram-positive bacteria that carry genes for superantigens, virulence factors, resistance certain metabolic functions. They have intimate relationships with temperate phages involving phage-induced excision, replication efficient packaging special small-headed infective phage-like particles, resulting very high transfer frequencies. generally contain 18-22 ORFs. We systematically inactivated each of these ORFs determined their...
ABSTRACT Staphylococcus aureus pathogenicity islands (SaPIs) have an intimate relationship with temperate staphylococcal phages. During phage growth, SaPIs are induced to replicate and efficiently encapsidated into special small heads commensurate their size. We analyzed by amino acid sequencing mass spectrometry the protein composition of specific SaPI particles. This has enabled identification major capsid tail proteins a putative portal protein. As expected, all these were encoded....
A robust, sensitive, and time-competitive system to detect Candida albicans in less than 30 min clinical samples based capped nanoporous anodic alumina (NAA) is developed. In the proposed design, NAA pores are loaded with rhodamine B then blocked an oligonucleotide that able recognize C. DNA. The material shows negligible cargo release, whereas dye delivery selectively accomplished when genomic DNA from present. This procedure has been successfully applied patients infected this yeast. When...
An inhibitor of α-amylase was isolated and purified from an extract white kidney beans ( Phaseolus vulgaris ). The acute oral administration the (50 mg/kg body weight) to adult Wistar rats together with a starch load (2 g/kg weight suspended in NaCl (9 g/l)) reduced increase glycaemia over basal value (NaCl, 222 (SEM 49); inhibitor, 145 16) mmol/l×180 min; P <0.05) without modifying insulin response. On administering orally dissolved for 21 d fed on standard diet, decline observed values...
A purified pancreatic alpha-amylase inhibitor (alpha-AI) from white beans (Phaseolus vulgaris) was administered orally (100 mg/kg body weight dissolved in 9 g NaCl/l) for 22 d to non-diabetic (ND) and type 2 diabetic (neonatal diabetes models n0-STZ n5-STZ) male Wistar rats. Mean glycaemia (mmol/l) declined day 4 of the alpha-AI administration ND rats (5.48 (sem 0.08) v. 4.39 0.13); P<0.05), (7.94 0.42) 5.56 0.32); P<0.01) n5-STZ (17.34 2.58) 11.93 1.96)), until end treatment: (5.22 0.21)...
Staphylococcus aureus pathogenicity islands (SaPIs) are a group of related 15-17 kb mobile genetic elements that commonly carry genes for superantigen toxins and other virulence factors. The key feature their mobility is the induction SaPI excision replication by certain phages efficient encapsidation into specific small-headed phage-like infectious particles. Previous work demonstrated chromosomal integration depends on SaPI-encoded recombinase, Int. However, although involved in process,...