A5088235650- Eicosanoids and Hypertension Pharmacology
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Liver Disease and Transplantation
- Inflammatory mediators and NSAID effects
- Cholesterol and Lipid Metabolism
- Biochemical and Structural Characterization
- Organ Transplantation Techniques and Outcomes
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Oral microbiology and periodontitis research
- Cancer, Hypoxia, and Metabolism
- Bacterial Genetics and Biotechnology
- Cardiac Ischemia and Reperfusion
- Pediatric Hepatobiliary Diseases and Treatments
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- RNA modifications and cancer
- Estrogen and related hormone effects
- Peroxisome Proliferator-Activated Receptors
- Antimicrobial Peptides and Activities
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Genomics, phytochemicals, and oxidative stress
Instituto de Biomedicina de Valencia
2010-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2020-2024
Instituto de Salud Carlos III
2020
NIHR Clinical Research Network
2019
Lymphatic Education & Research Network
2019
Universidad Cardenal Herrera CEU
2001-2007
ABSTRACT Identification of new genes involved in biofilm formation is needed to understand the molecular basis strain variation and pathogenic mechanisms implicated chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used generate biofilm-negative transposon (Tn917) insertion mutants. Two mutants were found with a significant decrease attachment inert surfaces (early adherence), intercellular adhesion, formation. The inserted at same locus both This ( bap...
ABSTRACT The enterococcal surface protein, Esp, is a high-molecular-weight protein of unknown function whose frequency significantly increased among infection-derived Enterococcus faecalis isolates. In this work, global structural similarity was found between Bap, biofilm-associated Staphylococcus aureus , and Esp. Analysis the relationship presence Esp-encoding gene ( esp ) biofilm formation capacity in E. demonstrated that highly associated P < 0.0001) with to form on polystyrene...
ABSTRACT Staphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability bacteria to produce biofilm. Here, we report relationship between chronic bovine mastitis and biofilm formation. We have classified S. isolates into three groups based on presence particular genetic elements required for formation: group 1 ( ica + bap ), 2 , negative), 3 negative, negative). Overall, animals naturally infected had lower milk somatic cell...
Sterol regulatory element binding proteins (SREBPs) regulate the expression of a number enzymes, which catalyze synthesis fatty acids, cholesterol, triglycerides, and phospholipids. SREBP1c is most relevant isoform in adult liver, its controlled by nutritional state. Transcriptional regulation studies into <i>SREBP1c</i> gene, performed last few years, have improved our knowledge variability signals that converge on promoter region. Insulin, cholesterol derivatives, T3 other endogenous...
ABSTRACT The adherence of Staphylococcus aureus to soluble proteins and extracellular-matrix components the host is one key steps in pathogenesis staphylococcal infections. S. presents a family adhesins called MSCRAMMs (microbial surface recognizing adhesive matrix molecules) that specifically recognize components. We examined influence biofilm-associated protein (Bap) expression on proteins, epithelial cell cultures, mammary gland sections colonization an vivo infection model. Bap-positive...
Summary We report the complete sequence of Staphylococcal pathogenicity island bovine 2 (SaPIbov2), encoding biofilm‐associated protein Bap. SaPIbov2 contains 24 open reading frames, including sip , which encodes a functional s taphylococcal i ntegrase p rotein. is bordered by 18 bp direct repeats. The integration site into chromosome lies at 3′ end gene GMP synthase. has extensive similarity to previously described islands Staphylococcus aureus . principal difference that toxin genes...
Liver ischemia and reperfusion injury (IRI) remains a serious clinical problem affecting liver transplantation outcomes. IRI causes up to 10% of early organ failure predisposes chronic rejection. Cyclooxygenase‐2 (COX‐2) is involved in different diseases, but the significance COX‐2 matter controversy. This study was designed elucidate role induction hepatocytes against IRI. In present work, hepatocyte‐specific transgenic mice (hCOX‐2‐Tg) their wild‐type (Wt) littermates were subjected...
Accumulation evidence links obesity-induced inflammation as an important contributor to the development of insulin resistance, which plays a key role in pathophysiology obesity-related diseases such type 2 diabetes and nonalcoholic fatty liver disease. Cyclooxygenase (COX)-1 -2 catalyze first step prostanoid biosynthesis. Because adult hepatocytes fail induce COX-2 expression regardless proinflammatory stimuli used, we have evaluated whether this lack under mild conditions might constitute...
Cyclooxygenase-2 (COX-2) is upregulated in many cancers, and the prostanoids synthesized increase proliferation, improve angiogenesis, inhibit apoptosis several tissues. To explore function of COX-2 liver, transgenic (Tg) mice were generated containing a fusion gene (LIVhCOX-2) consisting human cDNA under control ApoE promoter. Six lines developed; all them expressed LIVhCOX-2 transgene selectively hepatocytes. The Tg exhibited normal phenotype, increased levels PGE2 found due to...
ABSTRACT Neuronal specification is a protracted process that begins with the commitment of progenitor cells and culminates generation mature neurons. Many transcription factors are continuously expressed during this but it presently unclear how these modify their targets as transition through different stages specification. In olfactory bulb adult neurogenesis, factor PBX1 controls neurogenesis in survival migrating neuroblasts. Here, we show that, at later differentiation stages, also acts...
Cyclooxygenase 2 (COX-2) is a key enzyme in prostanoid biosynthesis. The constitutive hepatocyte expression of COX-2 has protective role hepatic ischemia-reperfusion (I/R) injury (IRI), decreasing necrosis, reducing reactive oxygen species (ROS) levels, and increasing autophagy antioxidant anti-inflammatory response. physiopathology IRI directly impacts mitochondrial activity, causing ATP depletion being the main source ROS. Using genetically modified mice expressing human (h-COX-2 Tg)...
A process of phase variation is described that affects the expression Bap (biofilm-associated protein) in Staphylococcus aureus. Upon subculture Bap-positive S. aureus strain V329 on Congo red agar, spontaneous smooth biofilm-negative colonies appeared at a low frequency (5 x 10(-4)). Northern blot analysis these variants with bap-specific gene probe showed transcription bap did not occur. However, DNA typing, Southern hybridization and sequencing show any differences between parent...
Insulin-induced gene 2 (INSIG2) and its homolog INSIG1 encode closely related endoplasmic reticulum proteins that regulate the proteolytic activation of sterol regulatory element-binding proteins, transcription factors activate synthesis cholesterol fatty acids in animal cells. Several studies have been carried out to identify INSIG2 genetic variants associated with metabolic diseases. However, few data published regarding regulation expression. Two Insig2 transcripts described rodents...
A diet high in fat and ethanol often results chronic metabolic disorder, hepatic steatosis, liver inflammation. Constitutive cyclooxygenase-2 (COX-2) expression could protect from fat-induced metabolism disturbance a murine model. In this study, we explored the influence of hCOX-2 transgenic [TG] to with ethanol-induced disorder injury using mouse animal model.12-week-old male (TG) or wild type mice (WT) were fed either liquid (HF+Eth) regular control (RCD) for 5 weeks (four groups: RCD/WT,...
The biochemical mechanisms of cell injury and myocardial death after infarction remain unresolved. Cyclooxygenase 2 (COX-2), a key enzyme in prostanoid synthesis, is expressed human ischemic myocardium dilated cardiomyopathy, but it absent healthy hearts. To assess the role COX-2 cardiovascular physiopathology, we developed transgenic mice that constitutively express functional cardiomyocytes under control α-myosin heavy chain promoter. These animals had no apparent phenotype were protected...
Abstract Background and Aims Cyclooxygenase‐2 (COX‐2) is involved in different liver diseases, but little known about the significance of COX‐2 cholestatic injury. This study was designed to elucidate role expression hepatocytes during pathogenesis obstructive cholestasis. Methods We used genetically modified mice constitutively expressing human hepatocytes. Transgenic ( hCOX‐2‐Tg ) their wild‐type Wt littermates were either subjected a mid‐abdominal laparotomy or common bile duct ligation...
On the Cover: Systemic hemoglobin exposure triggers blood-brain barrier (BBB) disruption and oxidative stress.Brain sections of guinea pigs transfused with polymerized cell-free were immunostained for markers stress (HO-1, green) BBB dysfunction (GFAP, red); nuclei counterstained Hoechst 33342 (blue).HO-1-positive pericytes/perivascular macrophages (green) are shown lining walls vessels diffuse enhanced GFAP reactivity.Original magnification, ϫ400.(See page 1316.