A5070164469- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Listeria monocytogenes in Food Safety
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Liver physiology and pathology
- Antimicrobial Resistance in Staphylococcus
- Yersinia bacterium, plague, ectoparasites research
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Organ Transplantation Techniques and Outcomes
- Mycobacterium research and diagnosis
- Microbial Metabolism and Applications
- Aldose Reductase and Taurine
- Oral microbiology and periodontitis research
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Cell Adhesion Molecules Research
- Bacterial Identification and Susceptibility Testing
- Ethnobotanical and Medicinal Plants Studies
- Forecasting Techniques and Applications
- Food Safety and Hygiene
- Essential Oils and Antimicrobial Activity
United States Food and Drug Administration
2015-2022
Center for Food Safety and Applied Nutrition
2015-2022
Washington University Medical Center
2011
George Washington University
2011
Background. Using a novel combination of whole-genome sequencing (WGS) analysis and geographic metadata, we traced the origins Salmonella Bareilly isolates collected in 2012 during widespread food-borne outbreak United States associated with scraped tuna imported from India. Methods. next-generation sequencing, sequenced complete genome 100 obtained patients who consumed contaminated product, natural sources, unrelated historically geographically disparate foods. Pathogen genomes were linked...
Epidemiological findings of a listeriosis outbreak in 2013 implicated Hispanic-style cheese produced by company A, and pulsed-field gel electrophoresis (PFGE) whole genome sequencing (WGS) were performed on clinical isolates representative collected from A environmental samples during the investigation. The results strengthened evidence for as vehicle. Surveillance sampling WGS 3 months later revealed that equipment purchased B yielded an isolate highly similar to all isolates. core...
ABSTRACT In 2014, the identification of stone fruits contaminated with Listeria monocytogenes led to subsequent a multistate outbreak. Simultaneous detection and enumeration L. were performed on 105 fruits, each weighing 127 145 g, collected from 7 lots. The results showed that 53.3% yielded (lower limit detection, 5 CFU/fruit), levels ranged 2,850 CFU/fruit, geometric mean 11.3 CFU/fruit (0.1 CFU/g fruit). Two serotypes, IVb-v1 1/2b, identified by combination PCR- antiserum-based serotyping...
Abstract Salmonella Dublin is a cattle-associated serovar sporadically causing disease in humans. S. strains isolated Brazil and other countries were analyzed to determine their phylogenetic relationships, the presence of genes, plasmids, genomic regions related virulence antimicrobial resistance genes repertoire, using WGS analyses. Illumina was used sequence genome 112 from humans (n = 82) animals 30) between 1983 2016. Furthermore, 87 analyzed. WGSNP analysis revealed three different...
Salmonella Enteritidis became the main serovar isolated from gastroenteritis cases in Brazil after 90's. In this study we used whole genome sequence analysis to determine phylogenetic relationships among a collection of strains identify possible genomic differences between pre and post-epidemic period. Also, compared our data available public domain other South American countries. Illumina technology was 256 over 48 year-period Brazil, comprising pre- Phylogenetic analyses revealed distinct...
Biofilms are a frequent cause of food contamination potentially pathogenic bacteria, such as Staphylococcus aureus . Given its vast role in human disease, the possible impact biofilm-producing S. isolates processing environment is evident. Sixty-nine collected from one firm following multiple staphylococcal poisoning outbreak investigations were utilized for this analysis. Strain evaluations performed to establish virulence determinants and evolutionary relationships using data generated by...
Hepatic stellate cells are embedded in the loose connective tissue matrix within space of Disse. This extracellular contains several basement membrane components including laminin, but its composition changes during liver injury because production found scar tissue. These and cell-extracellular interactions may play a key role hepatic cell transdifferentiation. In this communication we used early passages mouse (activated HSC/myofibroblasts) to study platelet-derived growth factor BB...
Abstract Whole genome sequencing of bacterial isolates has become a daily task in many laboratories, generating incredible amounts data. However, data acquisition is not an end itself; the goal to acquire high‐quality useful for understanding genetic relationships. Having method that could rapidly determine which available run metrics are most important indicators overall quality and having way monitor these during given would be extremely helpful this effect. Therefore, we compared various...
Listeriosis outbreaks are frequently multistate/multicountry outbreaks, underlining the importance of molecular typing data for several diverse and well-characterized isolates. Large-scale whole-genome sequencing studies on Listeria monocytogenes isolates from non-U.S. locations have been limited. Herein, we describe draft genome sequences 510 L. northern Italy different sources.
ABSTRACT Yersinia enterocolitica -like strains are usually understudied. In this work, we reported the draft genome sequences of two frederiksenii , intermedia and kristensenii isolated from humans, animals, food, environment in Brazil. These genomes will provide better molecular characterizations these species.
We report the genome sequence of Salmonella enterica subsp. serovar Give (CFSAN012622), isolated from imported chili powder in 2014. This contains genes previously reported to be specific only S. Enteritidis. strain shows a unique pulsed-field gel electrophoresis (PFGE) pattern clustering with Enteritidis (JEG X01.0005).
ABSTRACT Salmonella enterica serotype Enteritidis is a foodborne pathogen of global concern, because it frequently isolated from foods and patients. Draft genome sequences are reported here for 64 S . strains the intestines spleens mice caught live on chicken farms in U.S. Northeast. The availability these genomes provides baseline information genomic diversity during 1990s, when outbreaks traced to internal contamination eggs were prevalent.
Campylobacter species are the leading cause of gastroenteritis worldwide and an emerging threat in developing countries. Here, we report draft whole-genome sequences 51 jejuni 12 coli strains isolated from patients with Santiago, Chile.
Salmonella enterica serovar Dublin is a strongly adapted that causes enteritis and/or systemic disease in cattle and results high rates of mortality. Here, we report the draft genome sequences 112 S. strains isolated from humans animals Brazil. These will help enhance our understanding this
Foodborne pathogens have been implicated in illnesses worldwide. Here, we report the complete closed genome sequences of 28 bacterial strains belonging to 18 different species. These genomes belong known foodborne pathogens. The were by a combination long-read and short-read sequencing.
Salmonella enterica subsp. serovar Enteritidis emerged in the late 1980s as most isolated worldwide. Here, we report draft genomes of 256 S strains from humans, food, chickens, and farm environments Brazil. These will help enhance our understanding this
The number of Salmonella infection cases linked to pork products has increased. Pathogen presence in the feed mill environment is one many potential transmission routes into food production chain. Here, we describe draft genome sequences 57 enterica isolates from selected U.S. swine mills.
ABSTRACT To provide a better understanding of the diversity Salmonella enterica , we report assembled genome sequences 23 strains isolated from fecal samples cattle in Nigeria comprising 21 different serovars.
This SOP outlines the procedure for manual extraction of genomic DNA from live bacterial cells using Qiagen DNeasy Blood and Tissue Kit followed by quality control purified extracts Qubit purposes whole genome sequencing. is applicable to all GenomeTrakr laboratories with intent obtaining high quality, gram-negative or gram-positive use in subsequent sequencing protocols. Complete order: 1. Extraction (included protocol Automated Qiacube) Step-by-step procedures obtain isolates TSB 2....
Staphylococcus aureus bacteria are ranked among the top five foodborne pathogens in United States. Here, we report draft genome sequences of 62 S. isolates that originated from manufacturing environment an Illinois bakery and were associated with outbreaks between 2010 2011
Hepatic stellate cells (HSC) are embedded in a laminin‐rich matrix the Space of Disse normal liver. During injury HSC migrate to injured sites and produce scar collagen. One first proteins produced during liver is fibronectin. Therefore we investigated role laminin, fibronectin its receptors migration. METHODS We used mouse treated with PDGF‐BB Northern Western analysis study expression dystroglycan (DG) α5‐integrin (α5‐Int), respective for laminin (LN) (FN). also confocal microscopy...