A5062239133- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Environmental Toxicology and Ecotoxicology
- Advanced biosensing and bioanalysis techniques
- Advanced Proteomics Techniques and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- SARS-CoV-2 and COVID-19 Research
- Identification and Quantification in Food
- Pharmaceutical and Antibiotic Environmental Impacts
- Aquaculture disease management and microbiota
- Fullerene Chemistry and Applications
- Marine Toxins and Detection Methods
- Toxic Organic Pollutants Impact
- Marine and coastal ecosystems
- Molecular Biology Techniques and Applications
- Aquaculture Nutrition and Growth
- Photoreceptor and optogenetics research
- Gene expression and cancer classification
- Physiological and biochemical adaptations
- Reproductive biology and impacts on aquatic species
- Biomedical and Engineering Education
- Health and Medical Research Impacts
- Marine and coastal plant biology
- Ultrasound and Hyperthermia Applications
- Animal testing and alternatives
Baylor University
2020-2025
Aquatic Systems (United States)
2020-2025
University of South Carolina
2024-2025
University of Plymouth
2016-2019
Ecosystèmes, Biodiversité, Evolution
2019
Glasgow Centre for Population Health
1997
Advanced in vitro culture from tissues of different origin includes three-dimensional (3D) organoid micro structures that may mimic conditions vivo. One example simple 3D is spheroids; ball shaped typically used as liver and tumour models. Oxygen critically important physiological processes, but difficult to quantify culture: the question arises, how small does a spheroid have be minimal micro-environment formation? This particular importance growing field based models for toxicological...
SARS-CoV-2 RNA detection in wastewater is being rapidly developed and adopted as a public health monitoring tool worldwide. With surveillance programs implemented across many different scales by stakeholders, it critical that data collected shared are accompanied an appropriate minimal amount of metainformation to enable meaningful interpretation use this new information source intercomparison datasets. While some databases for specific locally, regionally, nationally, internationally,...
ABSTRACT Wastewater surveillance has emerged as a crucial public health tool for population-level pathogen surveillance. Supported by funding from the American Rescue Plan Act of 2021, FDA‘s genomic epidemiology program, GenomeTrakr, was leveraged to sequence SARS-CoV-2 wastewater sites across United States. This initiative required evaluation, optimization, development, and publication new methods analytical tools spanning sample collection through variant analyses. Version-controlled...
Due to its increasing affordability and efforts understand transcriptional responses of organisms biotic abiotic stimuli, transcriptomics has become an important tool with significant impact on toxicological investigations hazard risk assessments, especially during development application new approach methodologies (NAMs). Data generated using transcriptomic have directly informed adverse outcome pathway frameworks, chemical biological read across, aided in the identification points...
Abstract The glucocorticoid receptor (GR) is present in almost every vertebrate cell and utilized many biological processes. Despite an abundance of mammalian data, the structural conservation cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving impetus advance development new approach methodologies (NAMs). Here we used silico, vitro, vivo methods elucidate a greater...
In 2012, 20 key questions related to hazard and exposure assessment environmental health risks of pharmaceuticals personal care products in the natural environment were identified. A decade later, this article examines current level knowledge around one lowest-ranking at that time, number 19: "Can nonanimal testing methods be developed will provide equivalent or better data compared with vivo methods?" The inclusion alternative replace, reduce, refine animal within regulatory context risk...
There is an acknowledged need for in vitro fish intestinal model to help understand dietary exposure chemicals the aquatic environment. The presence and use of such models however largely restrictive due technical difficulties culturing enterocytes general availability appropriate established cell lines particular. In this study, rainbow trout (Oncorhynchus mykiss) derived line (RTgutGC) was used as a surrogate "gut sac" method. To facilitate comparison, RTgutGC cells were grown monolayers...
This study aimed to assess the ecotoxicological effects of interaction fullerene (C
Anatoxin-a and its analogues are potent neurotoxins produced by several genera of cyanobacteria. Due in part to high toxicity potential presence drinking water, these toxins pose threats public health, companion animals the environment. It primarily exerts as a cholinergic agonist, with affinity at neuromuscular junctions, but molecular mechanisms which it elicits toxicological responses not fully understood. To advance understanding this cyanobacteria, proteomic characterization (DIA...
Wastewater-based epidemiology (WBE) provides an early warning and trend analysis approach for determining the presence of COVID-19 in a community complements clinical testing assessing population level, even as viral loads fluctuate. Here, we evaluate combinations two wastewater concentration methods (i.e., ultrafiltration composite supernatant-solid), four pre-RNA extraction modifications, three nucleic acid kits using different sampling locations. These consisted quarantine facility...
Anatoxin-a is a globally occurring, yet understudied, chiral cyanobacterial toxin that threatens public health and the environment. It has led to numerous dog. livestock bird poisonings although it been studied in rodent models, comparatively little research occurred aquatic species. To advance comparative toxicology understanding of this alternative vertebrate developing zebrafish fathead minnow were exposed environmentally relevant elevated levels (13-4400 μg/L) (+) anatoxin-a examine...
ABSTRACT A novel method for the establishment and long-term maintenance of ex vivo cultures from intestinal regions rainbow trout, Oncorhynchus mykiss (Walbaum), is reported. Adherence cells was observed within hours, epithelial island formation recorded at 48 h rapid proliferation with confluence achieved between 9-14 days. In addition to metabolic characterisation, basic morphology growing characterised using histology, immunofluorescence, transmission electron microscopy (TEM)...
Compared to two-dimensional (2D) cell culture, cellular aggregates or spheroids (3D) offer a more appropriate alternative in vitro system where individual cell-cell communication and micro-environment closely represent the vivo organ; yet we understand little of physiological conditions at this scale. The relationship between spheroid size oxygen microenvironment, an important factor influencing metabolic capacity cells, was first established using fish intestine derived RTgutGC line....
Though anatoxin-a (antx-a) is a globally important cyanobacterial neurotoxin in inland waters, information on sublethal toxicological responses of aquatic organisms limited. We examined influences (±) antx-a (11-3490 μg/L) photolocomotor behavioral and gene transcription associated with neurotoxicity, oxidative stress hepatotoxicity, two the most common alternative vertebrate fish models, Danio rerio (zebrafish) Pimephales promelas (fathead minnow). selected environmentally relevant...
This study aimed to assess the ecotoxicological effects of interaction fullerene (C60) and benzo[a]pyrene (B[a]P) on marine mussel, Mytilus galloprovincialis. The uptake nC60, B[a]P mixtures nC60 into tissues was confirmed by GC-MS, LC-HRMS ICP-MS. Biomarkers DNA damage as well proteomics analysis were applied unravel toxic effect C60. Antagonistic responses observed at genotoxic proteomic level. Differentially expressed proteins (DEPs) only identified in single exposure mixture groups...