A5019638261- Helicobacter pylori-related gastroenterology studies
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Veterinary medicine and infectious diseases
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Applications in Construction Materials
- Corrosion Behavior and Inhibition
- Genomics and Phylogenetic Studies
- Galectins and Cancer Biology
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Trace Elements in Health
- Parasites and Host Interactions
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Listeria monocytogenes in Food Safety
- Eosinophilic Esophagitis
- Pancreatic and Hepatic Oncology Research
- Helminth infection and control
- Microbial Metabolites in Food Biotechnology
- Enterobacteriaceae and Cronobacter Research
- Bacterial Genetics and Biotechnology
University of Surrey
2016-2025
Liverpool School of Tropical Medicine
2021
Norwich Research Park
2007-2016
Quadram Institute
2007-2016
Vrije Universiteit Amsterdam
1999-2014
Norwich University
2013
Erasmus MC
2002-2010
Erasmus University Rotterdam
2002-2007
National Institute for Biological Standards and Control
2004
Ziekenhuis Bethesda
2004
ABSTRACT The expression of iron-regulated systems in gram-negative bacteria is generally controlled by the Fur protein, which represses transcription promoters using Fe 2+ as a cofactor. Mutational analysis Campylobacter jejuni fur gene was carried out generation set mutant copies had kanamycin or chloramphenicol resistance introduced into regions encoding N and C termini protein. mutated genes were recombined C. NCTC 11168 chromosome, putative mutants confirmed Southern hybridization....
The microaerophilic human pathogen Campylobacter jejuni is the leading cause of food-borne bacterial gastroenteritis in developed world. During transmission through food chain and environment, organism must survive stressful environmental conditions, particularly high oxygen levels. Biofilm formation has been suggested to play a role survival this organism. In work we show that C. NCTC 11168 biofilms more rapidly under food-chain-relevant aerobic conditions (20% O(2)) than microaerobic (5%...
Campylobacter jejuni is a major human enteric pathogen that displays genetic variability via genomic reorganization and phase variation. This can adversely affect the outcomes reproducibility of experiments. C. strain 81116 (NCTC11828) has been suggested to be genetically stable (G. Manning, B. Duim, T. Wassenaar, J. A. Wagenaar, Ridley, D. G. Newell, Appl. Environ. Microbiol. 67:1185-1189, 2001), amenable manipulation, infective for chickens. Here we report finished annotated genome sequence 81116.
ABSTRACT The bacterial pathogen Campylobacter jejuni is primarily transmitted via the consumption of contaminated foodstuffs, especially poultry meat. In food processing environments, C. required to survive a multitude stresses and requires use specific survival mechanisms, such as biofilms. An initial step in biofilm formation attachment surface. Here, we investigated effects chicken meat exudate (chicken juice) on surface formation. Supplementation brucella broth with ≥5% juice resulted...
Salmonella enterica is a significant foodborne pathogen, which can be transmitted via several distinct routes, and reports on acquisition of antimicrobial resistance (AMR) are increasing. To better understand the association between human clinical isolates potential environmental/animal reservoirs, whole genome sequencing (WGS) was used to investigate epidemiology AMR patterns within from two adjacent US states.WGS data 200 S. recovered (n = 44), swine 32), poultry 22), farm environment 102)...
Avian Pathogenic E. coli (APEC) is the causative agent of avian colibacillosis, resulting in economic losses to poultry industry through morbidity, mortality and carcass condemnation, impacts welfare poultry. Colibacillosis remains a complex disease manage, hampered by diagnostic classification strategies for that are inadequate defining APEC. However, increased accessibility whole genome sequencing (WGS) technology has enabled phylogenetic approaches be applied genomic characterization most...
ABSTRACT Microaerophiles like Campylobacter jejuni must resist oxidative stresses during transmission or infection. Growth of C. 81116 under iron limitation greatly increased the expression two polypeptides 26 and 55 kDa. The identification these proteins by N-terminal amino acid sequencing showed both to be involved in defense against stress. 55-kDa polypeptide was identical catalase (KatA), whereas N terminus 26-kDa homologous a Helicobacter pylori protein. gene encoding protein cloned,...
Expression of the peroxide stress genes alkyl hydroperoxide reductase (ahpC) and catalase (katA) microaerophile Campylobacter jejuni is repressed by iron. Whereas iron repression in gram-negative bacteria usually carried out Fur protein, previous work showed that this not case C. jejuni, as these are still a fur mutant. An open reading frame encoding homolog (designated PerR for "peroxide regulator") was identified genome sequence jejuni. The perR gene disrupted kanamycin resistance cassette...
Intracellular iron homeostasis is a necessity for almost all living organisms, since both restriction and overload can result in cell death. The ferric uptake regulator protein, Fur, controls most Gram-negative bacteria. In the human gastric pathogen Helicobacter pylori , Fur thought to have acquired extra functions compensate relative paucity of regulatory genes. To identify H. genes regulated by we used DNA array-based transcriptional profiling with RNA isolated from 26695 wild-type fur...
Maintaining metal homeostasis is crucial for the adaptation of Helicobacter pylori to gastric environment. Iron, copper, and nickel has recently been demonstrated be required establishment H. infection in animal models. Here we demonstrate that HP0969-0971 gene cluster encoding Czc-type export pump homologs HP0969, HP0970, pylori-specific protein HP0971 forms part a novel resistance determinant, which colonization modulation urease activity. Insertional mutagenesis HP0971, or HP0969 genes...
Expression of the Helicobacter pylori outer membrane protein HopH is regulated by phase variation within a CT dinucleotide repeat motif hopH gene.To investigate importance for bacterial pathogenicity, we performed detailed functional genomic and population-based genetic characterization this contingency locus.Sequencing in H. strains from 58 patients revealed that "on" genotype linked to virulence determinants, such as vacAs1, vacAm1, babA2, and, most strongly, cagA genotypes. mutagenesis...
ABSTRACT Maintaining iron homeostasis is a necessity for all living organisms, as free augments the generation of reactive oxygen species like superoxide anions, at risk subsequent lethal cellular damage. The iron-responsive regulator Fur controls metabolism in many bacteria, including important human pathogen Helicobacter pylori , and thus directly or indirectly involved regulation oxidative stress defense. Here we demonstrate that direct H. iron-cofactored dismutase SodB, which essential...
Campylobacter jejuni, a major food-borne intestinal pathogen, preferentially utilizes few specific amino acids and some organic such as pyruvate L- D-lactate carbon sources, which may be important for growth in the avian mammalian gut. Here, we identify enzymatic basis C. jejuni on L-lactate. Despite presence of an annotated gene fermentative lactate dehydrogenase (cj1167), no evidence excretion could obtained NCTC 11168, inactivation cj1167 did not affect source. Instead, L-lactate...
Biofilms make an important contribution to survival and transmission of bacterial pathogens in the food chain. The human pathogen Campylobacter jejuni is known form biofilms vitro chain-relevant conditions, but exact roles composition extracellular matrix are still not clear. Extracellular DNA has been found many can be a major component matrix. Here we show that also C. biofilm when attached stainless steel surfaces, aerobic conditions on conditioned surfaces. Degradation by exogenous...
Many bacterial genomes are highly variable but nonetheless typically published as a single assembled genome. Experiments tracking genome evolution have not looked at the variation present given point in time. Here, we analyzed mouse-passaged
Summary Campylobacter jejuni and coli are zoonotic pathogens once considered asaccharolytic, but now known to encode pathways for glucose fucose uptake/metabolism. For C. , strains with the fuc locus possess a competitive advantage in animal colonization models. We demonstrate that this is present > 50% of genome‐sequenced prevalent livestock‐associated isolates both species. To better understand how these campylobacters sense nutrient availability, we examined biofilm formation...
The Center for Disease Control and Prevention identifies antimicrobial resistant (AMR) Campylobacter as a serious threat to U.S. public health due high community burden, increased transmissibility, limited treatability. National Antimicrobial Resistance Monitoring System (NARMS) plays an important role in surveillance of AMR bacterial pathogens humans, food animals retail meats. This study investigated C . coli jejuni from live animals, poultry carcasses at production, meat North Carolina...
Disentangling the impact of weather on transmission infectious diseases is crucial for health protection, preparedness and prevention. Because factors are co-incidental partly correlated, we have used geography to separate out individual parameters other seasonal variables using campylobacteriosis as a case study. Campylobacter infections found worldwide most common bacterial food-borne disease in developed countries, where they exhibit consistent but country specific seasonality. We novel...
ABSTRACT Homologs of the ferric uptake regulator Fur and iron storage protein ferritin play a central role in maintaining homeostasis bacteria. The gastric pathogen Helicobacter pylori contains an iron-induced prokaryotic (Pfr) which has been shown to be involved protection against metal toxicity homolog not functionally characterized H. . Analysis isogenic fur -negative mutant revealed that is required for metal-dependent regulation ferritin. Iron starvation, as well medium supplementation...