A5074448565- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Animal Nutrition and Physiology
- Food Safety and Hygiene
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Veterinary medicine and infectious diseases
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Bacterial Identification and Susceptibility Testing
- Aquaculture disease management and microbiota
- Animal Virus Infections Studies
- Probiotics and Fermented Foods
- Fecal contamination and water quality
- Identification and Quantification in Food
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Animal Behavior and Welfare Studies
North Carolina State University
2015-2024
U.S. National Poultry Research Center
1999-2018
Agricultural Research Service
2007-2016
United States Department of Agriculture
2007-2016
Russell Biotech (United States)
2014
United States Food and Drug Administration
2002-2010
Atlanta Research and Education Foundation
2010
University of Georgia
2010
CNR de la Résistance aux Antibiotiques
2008
National Animal Disease Center
1994-2004
Food animals in the United States are often exposed to antimicrobials treat and prevent infectious disease or promote growth. Many of these identical closely resemble drugs used humans. Precise figures for quantity not publicly available States, estimates vary widely. Antimicrobial resistance has emerged zoonotic enteropathogens (e.g., Salmonella spp., Campylobacter spp.), commensal bacteria Escherichia coli, enterococci), bacterial pathogens Pasteurella, Actinobacillus but prevalence...
ABSTRACT The 16S rRNA gene has previously been used to develop genus-specific PCR primers for identification of enterococci. In addition, the superoxide dismutase ( sodA ) identified as a potential target species differentiation this study, Enterococcus developed by Deasy et al. (E1/E2) were incorporated with species-specific based upon development multiplex PCR. This assay provides simultaneous genus and 23 enterococci using seven different reaction mixtures. Accuracy was determined...
Transmission of Salmonella typhimurium in swine is traditionally believed to occur by the fecal-oral route, with invasion through intestinal wall and Peyer's patches. However, involvement upper respiratory tract may be equally important. An esophagotomy was performed on 6- 8-week-old pigs. Esophagotomized pigs were challenged intranasally 10(9) CFU S. cells necropsied at 3, 6, 12, 18 h postinoculation (p.i.). By 3 p.i., recovered from cecum, colon, head, thoracic tissues middle ileum...
Salmonella enterica, a leading cause of food-borne gastroenteritis worldwide, may be found in any raw food animal, vegetable, or fruit origin. serovars differ distribution, virulence, and host specificity. enterica serovar Kentucky, though often the supply, is less commonly isolated from ill humans. The multidrug-resistant isolate S. Kentucky CVM29188, chicken breast sample 2003, contains three plasmids (146,811 bp, 101,461 46,121 bp), two which carry resistance determinants (pCVM29188_146...
A multiplex PCR method has been developed to differentiate between the most common clinical serotypes of Salmonella enterica subsp. encountered in Washington State and United States general. Six genetic loci from S. serovar Typhimurium four Typhi were used create an assay consisting two five-plex PCRs. The assays gave reproducible results with 30 different that represent isolates enterica. Of these, 22 unique amplification patterns compared each other 8 grouped into pairs. These further...
Abstract Multidrug-resistant (MDR) Salmonella enterica has been deemed a high-priority pathogen by the World Health Organization. Two hundred and sixty-four isolates recovered over 16-year period (2000 to 2016) from poultry swine production chains, in Brazil, were investigated whole-genome sequencing (WGS). Most international lineages belonging 28 serovars, including, S . serovars Schwarzengrund ST96, Typhimurium ST19, Minnesota ST548, Infantis ST32, Heidelberg ST15, Newport ST45,...
Recognizing the importance of antimicrobial resistance and need for veterinarians to aid in efforts maintaining usefulness drugs animals humans, Board Regents American College Veterinary Internal Medicine charged a special committee with responsibility drafting this position statement regarding drug use veterinary medicine. The Committee believes that are obligated balance well‐being under their care protection other public health. Therefore, if an animal's medical condition can be...
Several methods were evaluated for collecting fecal and intestinal samples from wild birds found near broiler chicken houses. A few cloacal swabs obtained European starlings house sparrows. Most of the collected consisted bird droppings on or Samples each four farms a integrator during grow-out cycle: cycle in summer farm A, fall B, spring, summer, fall, winter C D. Of 25 July-August 1997, 24% positive Salmonella spp., 4% Campylobacter jejuni, 28% Clostridium perfringens. nine B...
ABSTRACT Salmonella enterica serotype typhimurium ( S. ) DT104 (DT104) first emerged as a major pathogen in Europe and is characterized by its pentadrug-resistant pattern. It has also been associated with outbreaks the United States. The organism typically carries resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, tetracycline. mechanism of chloramphenicol was determined producing antibiotic-resistant Escherichia coli host strain clones from DNA. DNA...
Campylobacter coli is a food-borne pathogen associated increasingly with human gastroenteritis. C. has high prevalence in swine, but isolated also from cattle and poultry. Multilocus sequence typing (MLST) systems have been developed to differentiate strains. Although substantial allelic diversity was identified across all seven MLST loci, no correlations were made two previous studies between allele or type (ST) the source of organism. However, this may be due either relatively small number...
ABSTRACT Molecular characterization (e.g., DNA-based typing methods) of Salmonella isolates is frequently employed to compare and distinguish clinical recovered from animals patients with food-borne disease nosocomial infections. In this study, we compared the abilities different phenotyping genotyping methods enterica serovar Typhimurium food animal sources. One hundred twenty-eight S. strains isolated cattle, pigs, chickens, turkeys or derived products were characterized using pulsed-field...
The contribution of dogs and cats as reservoirs antimicrobial resistant enterococci remains largely undefined. This is increasingly important considering the possibility transfer bacteria from companion animals to human host. In this study, veterinary clinics were screened for presence enterococci.A total 420 isolated nasal, teeth, rectal, belly hindquarters sites 155 121 three in Athens, GA. Eighty per cent (124 out 155) 60% (72 121) positive enterococci. From number dog samples (n = 275),...
ABSTRACT Due to emerging resistance traditional antimicrobial agents, such as ampicillin, trimethoprim-sulfamethoxazole, and chloramphenicol, azithromycin is increasingly used for the treatment of invasive Salmonella infections. In present study, 696 isolates non-Typhi collected from humans, food animals, retail meats in United States were investigated susceptibility azithromycin. Seventy-two enterica serotype Typhi humans also tested. For each isolate, MICs 15 other agents determined by...
Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety genes beneficial to the host organism, like those responsible for antimicrobial resistance. The objective this study was characterize collection 437 Salmonella enterica isolates from different animal sources their resistance phenotypes and plasmid replicon types and, in some cases, by pulsed-field gel electrophoresis (PFGE) an effort learn more about distribution multidrug relation types. A...
ABSTRACT The main goal of this study was to determine the prevalence methicillin-resistant Staphylococcus aureus (MRSA), particularly livestock-associated MRSA (LA-MRSA) in pigs and pork. genotypic relatedness isolates on farm, at slaughter, retail level assessed. Paired nasal perianal swab samples were collected from 10 cohorts market-age (24 per cohort) carcasses slaughterhouse, pork retail. Staphylococci isolated using selective enrichment method. Isolates tested for antimicrobial...
Hen housing for commercial egg production continues to be a societal and regulatory concern. Controlled studies have examined various aspects of safety, but comprehensive assessment hen systems in the US has not been conducted. The current study is part holistic, multidisciplinary comparison diverse conventional cage, enriched colony cage-free aviary focuses on environmental microbiology. Environmental swabs eggshell pools were collected from all during 4 periods. Total aerobes coliforms...