A5076105740- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Animal Nutrition and Physiology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Food Safety and Hygiene
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Inactivation Methods
- Vibrio bacteria research studies
- Essential Oils and Antimicrobial Activity
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Veterinary medicine and infectious diseases
- Escherichia coli research studies
- Fecal contamination and water quality
- Gut microbiota and health
- Livestock and Poultry Management
- Enterobacteriaceae and Cronobacter Research
- Helicobacter pylori-related gastroenterology studies
- Aquaculture disease management and microbiota
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Meat and Animal Product Quality
- Bacterial biofilms and quorum sensing
U.S. National Poultry Research Center
2016-2025
Agricultural Research Service
2016-2025
United States Department of Agriculture
2009-2024
Eastern Regional Research Center
2010
Quality Research
2000-2005
New York University
2003
Vanderbilt University
2003
Molecular Microbiology and Genomics Consultants (Germany)
2003
University of Georgia
1991
Food Safety and Inspection Service
1991
We compared nucleotide usage pattern conservation for related prokaryotes by examining the representation of DNA tetranucleotide combinations in 27 representative microbial genomes. For each organisms studied, departures from expectations (TUD) were shared between using both Markov chain analysis and a zero-order method. Individual strains, multiple chromosomes, plasmids, bacteriophages share TUDs within species. varied coding noncoding DNA. Grouping based on TUD profiles resulted...
Comparison of the entire coding sequence flaA (1,764 nucleotides) from 15 isolates Campylobacter jejuni showed two regions high variability, one region approximately base positions 700 to 1,450 and a short variable (SVR) 450 600. Parsimony analysis SVR sequences yielded dendrogram similar that which was derived by gene. PCR used generate templates, sequenced with primers constructed hybridize conserved flanking sequences. The SVRs 22 C. four outbreaks have been well characterized larger...
Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS), quantitative-PCR assays, network analysis to profile the poultry-associated microbiome pathogens at various stages of commercial poultry production from farm consumer. Analysis longitudinal data following two flocks through processing showed a core containing multiple sequence types most closely related genera known be...
<ns4:p><ns4:bold>Background: </ns4:bold>Most publicly available genomes of <ns4:italic>Salmonella enterica</ns4:italic> are from human disease in the US and UK, or domesticated animals US.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>Here we describe a historical collection 10,000 strains isolated between 1891-2010 73 different countries. They encompass broad range sources, ranging rivers through reptiles to diversity all <ns4:italic>S. enterica </ns4:italic>isolated on island Ireland 2000...
We identified a novel serotype 1/2a outbreak strain and 2 epidemic clones of Listeria monocytogenes while investigating foodborne listeriosis associated with consumption cantaloupe during 2011 in the United States. Comparative analyses strains worldwide are essential to identification clones.
Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety genes beneficial to the host organism, like those responsible for antimicrobial resistance. The objective this study was characterize collection 437 Salmonella enterica isolates from different animal sources their resistance phenotypes and plasmid replicon types and, in some cases, by pulsed-field gel electrophoresis (PFGE) an effort learn more about distribution multidrug relation types. A...
<ns3:p><ns3:bold>Background: </ns3:bold>Most publicly available genomes of <ns3:italic>Salmonella enterica</ns3:italic> are from human disease in the US and UK, or domesticated animals US.</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>Here we describe a historical collection 10,000 strains isolated between 1891-2010 73 different countries. They encompass broad range sources, ranging rivers through reptiles to diversity all <ns3:italic>S. enterica </ns3:italic>isolated on island Ireland 2000...
ABSTRACT Helicobacter pylori strains show both geographic and disease-associated allelic variation. We investigated the diversity present in two genes, babA babB , which are members of a paralogous family outer membrane proteins. Eleven within single H. strain, predicted to encode proteins with substantial N- C-terminal similarity each other, were classified as paralogues. In their central regions, most less than 54% related one another. Examining regions 42 from different locales, we...
Multidrug-resistant (MDR) Salmonella enterica serovar Typhimurium is the most prevalent penta-resistant isolated from animals by U.S. National Antimicrobial Resistance Monitoring System. Penta-resistant isolates are often resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline. To investigate MDR in (including variant 5-), one isolate each cattle, poultry, swine with at least tetracycline phenotype were selected for year 1997 2007 (n = 33) microarray...
A survey of 2,003 cecal content samples from chickens, turkeys, cattle, and swine at slaughter facilities in the United States was conducted to estimate prevalence mcr-1 gene conferring resistance colistin Enterobacteriaceae Two had Escherichia coli with IncI2 plasmids bearing gene.
ABSTRACT Campylobacter jejuni clone SA is the major cause of sheep abortion and contributes significantly to foodborne illnesses in United States. Clone hypervirulent because its distinct ability produce systemic infection predominant role clinical abortion. Despite importance SA, little known about distribution epidemiological features cattle. Here we describe a prospective study on C. prevalence 35 feedlots 5 different states States retrospective analysis isolates collected by National...
ABSTRACT We present the draft genomic sequences of 65 isolates Campylobacter spp. (38 coli and 27 jejuni ) isolated from table egg-layer chickens, which are not generally associated with human transmission. Up to this time, there no publicly available laying hens.
ABSTRACT We present the draft genomic sequences of 58 isolates Campylobacter spp. (32 coli and 26 jejuni ) isolated from domestic ducks ( Anas platyrhynchos domesticus ). This provides a substantial increase in number publicly available farm-raised United States.
ABSTRACT In an attempt to develop a method discriminate among isolates of Listeria monocytogenes , the sequences all annotated genes from fully sequenced strain L. EGD-e (serotype 1/2a) were compared by BLASTn file unfinished genomic sequence ATCC 19115 4b). Approximately 7% matching demonstrated 90% or lower identity between two strains, and lowest observed was 80%. Nine ( hisJ cbiE truB ribC comEA purM aroE hisC addB ) in 80 group gyrB rnhB with approximately 97% selected for multilocus...
To understand the mechanisms and epidemiology of antimicrobial resistance (AR), genetic elements responsible must be identified. Due to myriad possible genes, a high-density genotyping technique is needed for initial screening. achieve this, AR genes in National Center Biotechnology Information GenBank database were identified by their annotations compiled into nonredundant list 775 genes. A DNA microarray was constructed 70mer oligonucelotide probes designed detect these encoding...
The resistance to cecal colonization by Campylobacter jejuni was assessed challenging three crossbred stocks of commercially available broiler chickens. These stocks, designated A, B, and C, were related as follows: Offspring from four pedigreed grandparent flocks used progenitors. Stock B derived cross-breeding 1 with 3. Stocks A C crossbreeds grandparents 2 3 4, respectively. gavaged into 48-hour-old chicks, using the same levels challenge dose for each different chicken stocks. Six days...
The aim of this study was to compare antimicrobial resistance profiles distinct types Listeria monocytogenes isolated from a commercial poultry cooking plant. One hundred fifty-seven L. isolates representing 14 different actA were tested for susceptibility 19 drugs using the broth microdilution method. All susceptible antimicrobials except ceftriaxone (153 [97%] intermediate or resistant), oxacillin with 2% NaCl (142 [90%] ciprofloxacin (59 [37%] tetracycline (5 [3%] clindamycin (43 [27%]...
Next-generation DNA sequencing is rapidly becoming a powerful tool for food animal management. One valuable use of this technology to re-examine long-standing observations performance differences associated with husbandry practices better understand how these may be modulated by the gastrointestinal (GI) microbiome. The influences environmental parameters such as air temperature and relative humidity on broiler chicken have commonly been observed, but GI microbiome respond seasonal changes...