A5059853164- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral gastroenteritis research and epidemiology
- Trace Elements in Health
- Bacteriophages and microbial interactions
- Animal Nutrition and Physiology
- Enterobacteriaceae and Cronobacter Research
- CRISPR and Genetic Engineering
- Veterinary medicine and infectious diseases
- Gut microbiota and health
- Microbial Metabolism and Applications
- Listeria monocytogenes in Food Safety
- Clostridium difficile and Clostridium perfringens research
- Fecal contamination and water quality
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Antimicrobial Peptides and Activities
U.S. National Poultry Research Center
2018-2025
United States Department of Agriculture
2018-2023
Agricultural Research Service
2018-2023
National Wildlife Research Center
2017
Animal and Plant Health Inspection Service
2017
ABSTRACT Campylobacter jejuni clone SA is the major cause of sheep abortion and contributes significantly to foodborne illnesses in United States. Clone hypervirulent because its distinct ability produce systemic infection predominant role clinical abortion. Despite importance SA, little known about distribution epidemiological features cattle. Here we describe a prospective study on C. prevalence 35 feedlots 5 different states States retrospective analysis isolates collected by National...
The chicken gastrointestinal tract harbors microorganisms that play a role in the health and disease status of host. cecum is part gut carries highest microbial densities, has longest residence time digesta, vital site for urea recycling water regulation. Therefore, provides rich environment bacteria to horizontally transfer genes between one another via mobile genetic elements such as plasmids bacteriophages. In this study, we used broiler model investigate antibiotic resistance can be...
ABSTRACT Broiler litter sampling has proven to be an effective method for determining the Salmonella status of a broiler chicken flock and understanding ecology prior harvest. In this study, we investigated within ( n = 224) from two commercial houses in United States. We employed culture enrichment methods quantitative polymerase chain reaction determine prevalence load utilized antimicrobial susceptibility testing whole-genome sequencing (WGS) characterize select isolates. Additionally,...
Salmonella enterica subsp. serovar Heidelberg (S. Heidelberg) is a clinically-important linked to food-borne illness, and commonly isolated from poultry. Investigations of large, multistate outbreak in the USA 2013 identified poultry litter (PL) as an important extra-intestinal environment that may have selected for specific S. strains. Poultry mixture bedding materials chicken excreta contains gastrointestinal (GI) bacteria, undigested feed, feathers, other origin. In this study, we...
Fostering a "balanced" gut microbiome through the administration of beneficial microbes that can competitively exclude pathogens has gained lot attention and use in human animal medicine. However, little is known about how affect horizontal gene transfer antimicrobial resistance (AMR). To shed more light on this question, we challenged neonatal broiler chicks raised reused chicken litter-a complex environment made up decomposing pine shavings, feces, uric acid, feathers, feed-with Salmonella...
The reported increase in antibiotic-resistant bacteria humans has resulted a major shift away from antibiotic use food animal production. This been driven by the assumption that removing antibiotics will select for susceptible bacterial taxa, which turn allow currently available arsenal to be more effective.
In this study, we conducted a longitudinal sampling of peanut hull-based litter from farm under "no antibiotics ever" program. Our objective was to determine broiler management practices and environmental factors that are associated with the occurrence food-borne pathogens (Salmonella Campylobacter) abundance commensal bacteria (Escherichia coli, Enterococcus spp., Staphylococcus spp.). Litter (n = 288) collected 4 houses over three consecutive flocks, starting complete house cleanout fresh...
is a leading cause of foodborne illness in the United States due to consumption contaminated or mishandled food products, often associated with chicken meat.
In this study, we investigated the dynamics of ceca and litter microbiome chickens from post-hatch through pre-harvest. To achieve this, six hundred one-day old Cobb 500 broiler chicks were raised on floor pens for 49 days in two separate houses. We performed short-read full-length sequencing bacterial 16S rRNA gene present meconium cecal samples collected over duration study. addition, determined antimicrobial resistance (AMR) phenotype Escherichia coli Enterococcus spp. isolated 49-day...
Several studies have investigated antimicrobial resistance (AMR) in Salmonella spp. and Escherichia coli isolated from hospitalized horses, but conducted on community-based populations of equids are limited. The factors associated with AMR these bacteria the general horse population not well understood. primary objective our study was to estimate prevalence describe susceptibility E. across United States. second identify associations between health management biosecurity practices AMR. Fecal...
We tested a diverse set of 500 isolates nontyphoidal Salmonella enterica subsp. from various animal, food, and human clinical sources for susceptibility to antimicrobials currently lacking epidemiological cutoff values (ECOFFs) by the European Committee on Antimicrobial Susceptibility Testing. A consortium five different laboratories each 100 isolates, using broth microdilution panels containing twofold dilutions ceftriaxone, cefepime, colistin determine minimum inhibitory concentrations...
ABSTRACT Salmonella enterica subsp. serovar Heidelberg ( S. Heidelberg) is a clinically-important linked to food-borne illness, and commonly isolated from poultry. Investigations of large, multistate outbreak in the USA 2013 identified poultry litter (PL) as an important extra-intestinal environment that may have selected for specific strains. Poultry mixture bedding materials chicken excreta contains gastrointestinal (GI) bacteria, undigested feed, feathers, other origin. In this study, we...
Salmonella infections are a leading cause of bacterial food-borne illness worldwide. Infections highly associated with the consumption contaminated food, and in particular, chicken meat. The severity depends on presence antimicrobial resistance genes virulence factors. While there many studies which have investigated strains isolated from postharvest samples, is gap our understanding genetic properties that influence persistence preharvest particular their makeup We used whole genome...
Abstract The chicken gastrointestinal tract harbors taxa of microorganisms that play a role in the health and disease status host. cecum is part gut carries highest microbial densities, has longest residence time digesta vital site for urea recycling water regulation. Therefore, provides rich environment bacteria to horizontally transfer genes between one another via mobile genetic elements such as plasmids bacteriophages. In this study, we used broiler model investigate antibiotic...
Abstract Antimicrobial resistance (AR) spread is a worldwide health challenge, stemming in large part, from the ability of microbes to share their genetic material through horizontal gene transfer (HGT). Overuse and misuse antibiotics clinical settings food production have been linked this increased prevalence AR. Consequently, public consumer concerns resulted remarkable recent reduction used for animal production. This driven by assumption that removing selective pressure will favor...
Abstract Campylobacter infections are a leading cause of bacterial diarrhea in humans globally. Infections due to consumption contaminated food products and highly associated with chicken meat, chickens being an important reservoir for . Here, we characterized the genetic diversity species detected broiler litter over three consecutive flocks determined their antimicrobial resistance virulence factor profiles. Antimicrobial susceptibility testing whole genome sequencing were performed on...
Abstract Salmonella infections are a leading cause of bacterial food-borne illness worldwide. Infections highly associated with the consumption contaminated food, and in particular, chicken meat. Understanding how management practices environmental factors influence populations broiler production may aid reducing risk humans. Utilizing whole genome sequencing antimicrobial heavy metal resistance, virulence factor plasmid identification, we have characterized genetic diversity enterica...
Several studies have explored antimicrobial resistance (AMR) among enteric bacteria isolated from hospitalized horses, but few been conducted on community-based populations of healthy equids. Additional knowledge patterns equids is needed to better understand AMR in veterinary medicine. Our study aimed estimate the prevalence fecal Escherichia coli and Salmonella spp. across United States. Fecal samples were collected 1,357 199 operations, 721 which cultured for E. . All screened via...
Abstract Fostering a ’balanced’ gut microbiome through the administration of beneficial microbes that can competitively exclude pathogens has gained lot attention and use in human animal medicine. However, little is known about how affect horizontal gene transfer antimicrobial resistance (AMR). To shed more light on this question, we challenged neonatal broiler chicks raised reused chicken litter – complex environment made up decomposing pine shavings, feces, uric acid, feathers, feed, with...
Highlights• Fecal shedding of Salmonella spp. in horses was low• Multidrug resistant were detected a limited number isolates• and E. coli concurrent rare with single sampling timepoint• Trimethoprim-sulfa sulfisoxazole resistance common coli• Antimicrobial higher from performance-type equid operations