A5033374590- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Fecal contamination and water quality
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Animal Nutrition and Physiology
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- CRISPR and Genetic Engineering
- Food Safety and Hygiene
- Identification and Quantification in Food
U.S. National Poultry Research Center
2017-2025
United States Department of Agriculture
2018-2023
Agricultural Research Service
2018-2023
Surface waters are important sources of water for drinking, industrial, agricultural, and recreational uses; hence, contamination by fecal, pathogenic, or antimicrobial resistant (AR) bacteria is a major environmental public health concern. However, very little data available on prevalence these in surface throughout watershed. This study aimed to characterize Escherichia coli present the Upper Oconee Watershed, mixed-use watershed Athens, GA, USA potential pathogenicity AR. E. were...
The reported increase in antibiotic-resistant bacteria humans has resulted a major shift away from antibiotic use food animal production. This been driven by the assumption that removing antibiotics will select for susceptible bacterial taxa, which turn allow currently available arsenal to be more effective.
In this study, we conducted a longitudinal sampling of peanut hull-based litter from farm under "no antibiotics ever" program. Our objective was to determine broiler management practices and environmental factors that are associated with the occurrence food-borne pathogens (Salmonella Campylobacter) abundance commensal bacteria (Escherichia coli, Enterococcus spp., Staphylococcus spp.). Litter (n = 288) collected 4 houses over three consecutive flocks, starting complete house cleanout fresh...
As the cases of Salmonella enterica infections associated with contaminated water are increasing, this study was conducted to address role surface as a reservoir S. serotypes. We sampled rivers and streams (n = 688) over 3-year period (2015 2017) in mixed-use watershed Georgia, USA, 70.2% total stream samples tested positive for Salmonella. A 1,190 isolates were recovered characterized by serotyping, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE). wide range...
As resistance to the β-lactam class of antibiotics has become a worldwide problem, multidrug-resistant (MDR) human (n = 243) and food animal 211) isolates from Lagos, Nigeria were further tested characterize β-lactamase-encoding genes plasmid replicons. Four (blaCMY, blaCTX-M, blaOXA, blaTEM) detected using PCR-based replicon typing, 13 17 different replicons identified subset MDR E. coli humans 48) animals 96), respectively. Replicon types FIB X2 in equal numbers (2/48; 4.2% each) isolates,...
The presence and transfer of plasmids from commensal bacteria to more pathogenic may contribute the dissemination antimicrobial resistance. However, prevalence bacteria, such as enterococci, in food animals remains largely unknown. In this study, diversity plasmid families multidrug-resistant (MDR; resistance three or antimicrobials) enterococci poultry carcasses were determined. Plasmid-positive MDR also tested for ability other using conjugation. Enterococcus faecalis (n = 98) faecium 696)...
Several studies have investigated antimicrobial resistance (AMR) in Salmonella spp. and Escherichia coli isolated from hospitalized horses, but conducted on community-based populations of equids are limited. The factors associated with AMR these bacteria the general horse population not well understood. primary objective our study was to estimate prevalence describe susceptibility E. across United States. second identify associations between health management biosecurity practices AMR. Fecal...
Abstract Salmonella detection and isolation rely on different selective enrichment media, which can influence serovars are detected. The objective of this study was to compare recovery from broiler carcass rinses using three protocols differential plating agars. Eight prechill carcasses were collected at a commercial slaughter facility. Each subjected whole rinse procedure in buffered peptone water (BPW). An aliquot the remaining incubated as pre‐enrichment before subculturing broths...
Abstract Many laboratories sampling foods for Salmonella are interested only in presence or absence of , so one colony may be selected. The objectives this study were to use two selective enrichment broths and agar plating media recovery from naturally contaminated broiler carcass rinsates evaluate these combinations on serotypes recovered each carcass. Broiler carcasses ( n = 52) a commercial processing plant prior chilling rinsed with buffered peptone water after incubation subcultured...
Abstract Antimicrobial resistance (AR) spread is a worldwide health challenge, stemming in large part, from the ability of microbes to share their genetic material through horizontal gene transfer (HGT). Overuse and misuse antibiotics clinical settings food production have been linked this increased prevalence AR. Consequently, public consumer concerns resulted remarkable recent reduction used for animal production. This driven by assumption that removing selective pressure will favor...
ABSTRACT Here, we present the draft genome sequences of eight streptogramin-resistant Enterococcus species isolated from animals and an environmental source in United States 2001 to 2004. Antimicrobial resistance genes were identified conferring macrolide-lincosamide-streptogramins, aminoglycosides, tetracyclines, beta-lactams, glycopeptides.
Abstract Detection of Salmonella on commercially processed broiler carcasses is essential to reduce the incidences human salmonellosis. This study was designed compare standard procedure for detection with two commercial screening methods. During seven separate visits processing plants, eight were obtained ( n = 112). Each carcass rinsed and rinsate according U.S. Department Agriculture (USDA), Food Safety Inspection Service (FSIS) protocol. Predetermined aliquots rinsates screened BAX®...
Several studies have explored antimicrobial resistance (AMR) among enteric bacteria isolated from hospitalized horses, but few been conducted on community-based populations of healthy equids. Additional knowledge patterns equids is needed to better understand AMR in veterinary medicine. Our study aimed estimate the prevalence fecal Escherichia coli and Salmonella spp. across United States. Fecal samples were collected 1,357 199 operations, 721 which cultured for E. . All screened via...
Highlights• Fecal shedding of Salmonella spp. in horses was low• Multidrug resistant were detected a limited number isolates• and E. coli concurrent rare with single sampling timepoint• Trimethoprim-sulfa sulfisoxazole resistance common coli• Antimicrobial higher from performance-type equid operations