A5047989587- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Food Safety and Hygiene
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Zoonotic diseases and public health
- Essential Oils and Antimicrobial Activity
- Microbial Inactivation Methods
- Identification and Quantification in Food
- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Botulinum Toxin and Related Neurological Disorders
- Enterobacteriaceae and Cronobacter Research
- Molecular Biology Techniques and Applications
- Hepatitis Viruses Studies and Epidemiology
- Fecal contamination and water quality
- Osteomyelitis and Bone Disorders Research
- Respiratory viral infections research
- Turtle Biology and Conservation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Antimicrobial Resistance in Staphylococcus
Centers for Disease Control and Prevention
2014-2024
Waterborne Environmental (United States)
2012-2023
United States Department of Health and Human Services
2016-2023
National Center for Emerging and Zoonotic Infectious Diseases
2012-2022
The Centers
2015-2019
Guangdong Provincial Center for Disease Control and Prevention
2015
CDC Foundation
2015
National Center for Infectious Diseases
2005
Background. Listeriosis, a life-threatening foodborne illness caused by Listeria monocytogenes, affects ∼ 2500 Americans annually. Between July and October 2002, an uncommon strain of L. monocytogenes outbreak listeriosis in 9 states. Methods. We conducted case finding, case-control study, traceback microbiological investigations to determine the extent source propose control measures. Case patients were infected with between November 2002 states, different strains. Outcome measures included...
PulseNet USA is the molecular surveillance network for foodborne disease in United States. The consists of state and local public health laboratories, as well food regulatory agencies, that follow PulseNet's standardized protocols to perform pulsed-field gel electrophoresis (PFGE) whole genome sequencing (WGS) analyze results using software. raw sequences are uploaded GenomeTrakr or bioprojects at National Center Biotechnology Information. PFGE patterns analyzed sequence data real time with...
The routine use of whole-genome sequencing (WGS) as part enteric disease surveillance is substantially enhancing our ability to detect and investigate outbreaks monitor trends. At the same time, it revealing never before vast complexity microbial human interactions that contribute outbreak ecology. Since WGS analysis primarily used characterize compare genomes with goal addressing epidemiological questions, must be interpreted in an context. In this article, we identify common challenges...
Abstract Invasive Cronobacter infections among infants are associated with severe neurologic disabilities and death. Early reports typically featured hospitalized preterm recognized contaminated powdered infant formula (PIF) as a transmission vehicle. To clarify recent epidemiology, we reviewed all cases of bloodstream infection or meningitis that were reported to the Centers for Disease Control Prevention in literature (1961–2018; n = 183). Most neonates (100/150 [67%]); 38% (42/112) died,...
ABSTRACT A multistate outbreak of listeriosis occurred in the United States 1998 with illness onset dates between August and December. The caused 108 persons residing 24 states 14 deaths four miscarriages or stillbirths. This was detected by public health officials Tennessee New York who observed significant increases over expected cases their states. Subsequently, Centers for Disease Control Prevention (CDC) began laboratory characterization clinical isolates Listeria monocytogenes...
Since 1996, PulseNet has served as the national laboratory-based surveillance system for rapid detection of outbreaks caused by foodborne bacterial pathogens in United States. For past two decades, pulsed-field gel electrophoresis was gold standard subtyping method tracked PulseNet. A new is now being implemented with introduction cost-effective whole genome sequencing (WGS) analysis all organisms This transformation a major undertaking that touches every functional aspect PulseNet,...
Salmonella enterica is one of the most common causes foodborne illness in United States. Although salmonellosis usually self-limiting, severe infections typically require antimicrobial treatment, and ceftriaxone, an extended-spectrum cephalosporin (ESC), commonly used both adults children. Surveillance conducted by National Antimicrobial Resistance Monitoring System (NARMS) has shown a recent increase ESC resistance among Heidelberg isolated from food animals at slaughter, retail meat,...
Worldwide, Salmonella spp. is a significant cause of disease for both humans and wildlife, with wild birds adapted to urban environments having different opportunities pathogen exposure, infection, transmission compared their natural conspecifics. Food provisioning by people may influence these factors, especially when high-density mixed species flocks aggregate. White Ibises (Eudocimus albus), an iconic Everglades in decline Florida, are becoming increasingly common urbanized areas south...
During the past decade, extended-spectrum cephalosporin resistance has increased among human isolates of Salmonella enterica serovar Heidelberg, fourth most common serotype in United States. We therefore characterized 54 Heidelberg with decreased susceptibility (minimum inhibitory concentrations >or=2 mg/L) to ceftriaxone or ceftiofur; 49 (90.7%) contained CMY-type beta-lactamase (bla(CMY)) gene. The bla(CMY)-positive demonstrated a high degree relatedness; 4 clusters (25 total) had...
Salmonellosis cases in the United States show distinct geographical trends, with southeast reporting among highest rates of illness. In state Georgia, USA, non-outbreak associated salmonellosis is especially high southern low-lying coastal plain. Here we examined distribution Salmonella enterica environmental waters and wildlife two watersheds, one Atlantic Coastal Plain (a case rate rural area) physiographic province Piedmont lower area). were isolated from regions compared for serovar...
Salmonella enterica is a leading cause of bacterial foodborne and zoonotic illnesses in the United States. For this study, we applied four different whole genome sequencing (WGS)-based subtyping methods: high quality single-nucleotide polymorphism (hqSNP) analysis, multilocus sequence typing using either all loci [wgMLST (all loci)] only chromosome-associated (chrom)], core (cgMLST) to dataset isolate sequences from 9 well-characterized outbreaks. each outbreak, evaluated genomic...
Introduction: Changes in food production and distribution have increased opportunities for foods contaminated early the supply chain to be distributed widely, increasing possibility of multistate outbreaks. In recent decades, surveillance systems foodborne disease been improved, allowing officials more effectively identify related cases trace an outbreak's source. Materials Methods: We reviewed outbreaks reported Centers Disease Control Prevention's Foodborne Outbreak Surveillance System...
Foodborne outbreak investigations have traditionally included the detection of a cluster illnesses first, followed by an epidemiologic investigation to identify food interest. The increasing use whole genome sequencing (WGS) subtyping technology for clinical, environmental, and isolates foodborne pathogens, ability share compare data on public platforms, present new opportunities earlier links between their potential sources. We describe process called sample-initiated retrospective (SIROIs)...
Campylobacter is a leading causing of bacterial foodborne and zoonotic illnesses in the USA. Pulsed-field gene electrophoresis (PFGE) 7-gene multilocus sequence typing (MLST) have been historically used to differentiate sporadic from outbreak isolates. Whole genome sequencing (WGS) has shown provide superior resolution concordance with epidemiological data when compared PFGE MLST during investigations. In this study, we evaluated for high-quality SNP (hqSNP), core (cg)MLST whole (wg)MLST...
Whole genome sequencing is replacing traditional laboratory surveillance methods as the primary tool to track and characterize clusters outbreaks of foodborne zoonotic pathogen
In January 2017, CDC identified a cluster of Salmonella enterica serotype Newport infections with isolates sharing an indistinguishable pulsed-field gel electrophoresis (PFGE) pattern, JJPX01.0010 (pattern 10), through PulseNet, the national molecular subtyping network for foodborne disease surveillance. This report summarizes investigation by CDC, state and local health agriculture departments, U.S. Department Agriculture's Food Safety Inspection Service (USDA-FSIS) discusses possible role...
A classification model is presented for rapid identification of Salmonella serotypes based on pulsed-field gel electrophoresis (PFGE) fingerprints. The was developed using random forest and support vector machine algorithms then applied to a database 45,923 PFGE patterns, randomly selected from all submissions CDC PulseNet 2005 2010. patterns included the top 20 most frequent 12 less various sources. prediction accuracies 32 ranged 68.8% 99.9%, with an overall accuracy 96.0% classification,...
High consumption rates and a multitude of brands make multistate foodborne outbreaks Salmonella infections associated with chicken challenging to investigate, but whole genome sequencing is powerful tool that can be used assist investigators. Whole pathogens isolated from clinical, environmental, food samples increasingly being in outbreak investigations determine unprecedented resolution how closely related these isolates are one another genetically. In 2014, federal state health officials...
In July 2021, the Colorado Department of Public Health and Environment (CDPHE) laboratory identified a cluster five Salmonella enterica serotype Thompson isolates related to one another within allele difference, using whole genome multilocus sequence typing (wgMLST). These isolates, submitted public health as is routine process for confirmatory testing Salmonella, were highly those in 2020 multistate investigation, during which traceback was conducted sushi-grade tuna salmon; common supplier...
Shiga toxins are produced by Shigella dysenteriae type 1 and certain strains of Escherichia coli . Three cases toxin–producing S. 4 were identified among travelers to the island Hispañola between 2002 2005. Clinical public health practitioners should be aware this newly strain.