A5013478374- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Pharmaceutical and Antibiotic Environmental Impacts
- Escherichia coli research studies
- Medical Imaging Techniques and Applications
- Probiotics and Fermented Foods
- Enterobacteriaceae and Cronobacter Research
- Veterinary medicine and infectious diseases
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Yersinia bacterium, plague, ectoparasites research
- Aquaculture disease management and microbiota
- Inflammatory Bowel Disease
- Cancer, Hypoxia, and Metabolism
- Microscopic Colitis
- Protein Tyrosine Phosphatases
- Nutrition, Genetics, and Disease
- Bacterial Genetics and Biotechnology
- Zoonotic diseases and public health
Statens Serum Institut
2009-2024
Technical University of Denmark
2017
Frederiksberg Hospital
2017
University of Copenhagen
2017
University College Cork
2013
Federal Institute for Risk Assessment
2010
The combination of virulence gene and antimicrobial resistance typing using DNA arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. We have now applied this technology 523 Salmonella enterica subsp. strains collected from various host sources public health veterinary institutes across nine European countries. strain set included the five predominant serovars isolated in Europe (Enteritidis, Typhimurium, Infantis, Virchow, Hadar). Initially, these were...
Abstract Hybridization between distantly related organisms can facilitate rapid adaptation to novel environments, but is potentially constrained by epistatic fitness interactions among cell components. The zoonotic pathogens C ampylobacter coli and . jejuni differ from each other around 15% at the nucleotide level, corresponding an average of nearly 40 amino acids per protein‐coding gene. Using whole genome sequencing, we show that a single C. lineage, which has successfully colonized...
<ns4:p><ns4:bold>Background: </ns4:bold>Most publicly available genomes of <ns4:italic>Salmonella enterica</ns4:italic> are from human disease in the US and UK, or domesticated animals US.</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>Here we describe a historical collection 10,000 strains isolated between 1891-2010 73 different countries. They encompass broad range sources, ranging rivers through reptiles to diversity all <ns4:italic>S. enterica </ns4:italic>isolated on island Ireland 2000...
Salmonella enterica serovar Agona has caused multiple food-borne outbreaks of gastroenteritis since it was first isolated in 1952. We analyzed the genomes 73 isolates from global sources, comparing five distinct with sporadic infections as well food contamination and environment. consists three lineages minimal mutational diversity: only 846 single nucleotide polymorphisms (SNPs) have accumulated non-repetitive, core genome evolved 1932 subsequently underwent a major population expansion...
This report describes one Salmonella isolate harbouring both mcr-1 and mcr-3. We also found nine other isolates positive for the plasmid-borne colistin resistance gene, The strains were isolated from patients in Denmark between 2009 2017 five of had travelled to Asia. In addition mcr-3, all blaTEM-1, strA, strB, sul2 tet(A) or tet(B), most blaCTX-M-55 qnrS.
<ns3:p><ns3:bold>Background: </ns3:bold>Most publicly available genomes of <ns3:italic>Salmonella enterica</ns3:italic> are from human disease in the US and UK, or domesticated animals US.</ns3:p><ns3:p> <ns3:bold>Methods: </ns3:bold>Here we describe a historical collection 10,000 strains isolated between 1891-2010 73 different countries. They encompass broad range sources, ranging rivers through reptiles to diversity all <ns3:italic>S. enterica </ns3:italic>isolated on island Ireland 2000...
Abstract Background Salmonella Infantis ( S . Infantis) is one of the most frequent serovars isolated from human cases salmonellosis and detected serovar animal food sources in Europe. The commonly associated with poultry there increasing concern over multidrug resistant clones spreading worldwide, as dominating are characterized by presence large plasmids carrying multiple resistance genes. Increasing knowledge population evolution important for understanding preventing further spread. In...
Prevention of the emergence and spread foodborne diseases is an important prerequisite for improvement public health. Source attribution models link sporadic human cases a specific illness to food sources animal reservoirs. With next generation sequencing technology, it possible develop novel source models. We investigated potential machine learning predict reservoir from which bacterial strain isolated salmonellosis case originated based on whole-genome sequencing. Machine methods recognize...
Escherichia coli have been found in increased numbers tissues from patients with Inflammatory Bowel Disease (IBD) and adherent-invasive E. resected ileum Crohn's disesae. This study aimed to characterize possible differences phylogenetic group (triplex PCR), extraintestinal pathogenic (ExPEC) genes multilocus sequence type (MLST) between strains isolated IBD past or present involvement of the left side colon controls. Fecal samples were collected 18 10 healthy activity was evaluated by...
Whole-genome sequencing is rapidly replacing current molecular typing methods for surveillance purposes. Our study evaluates core-genome single-nucleotide polymorphism analysis outbreak detection and linking of sources Salmonella enterica serovar Typhimurium its monophasic variants during a 7-month period in Denmark. We reanalyzed defined 8 previously characterized outbreaks from the phylogenetic relatedness isolates, epidemiologic data, food traceback investigations. All were identified, we...
In April 2019, a cross-border outbreak of Yersinia entercolitica O3 was identified in Sweden and Denmark confirmed using whole genome sequencing. Close collaboration with representatives from human food authorities helped direct resources investigations. Combined epidemiological trace-back investigations pointed to imported fresh spinach as the vehicle highlight that other vehicles Y. enterocolitica outbreaks than pork should be considered.
The European epidemic monophasic variant of
Aquaculture systems are widely recognised as hotspots for horizontal gene transfer, and the need screening bacteria carrying antimicrobial resistance genes in aquaculture is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, A. nosocomialis) resistant to colistin originating from retailed products imported Vietnam Czech Republic. The mcr-1.1 was found located on plasmid types IncHI2, IncI2,...
Journal Article Multilocus sequence typing performed on Campylobacter coli isolates from humans, broilers, pigs and cattle originating in Denmark Get access E. Litrup, Litrup Statens Serum Institut, Copenhagen S, Artillerivej 5, 2300 Copenhagen, Denmark. E‐mail: evl@ssi.dk Search for other works by this author on: Oxford Academic Google Scholar M. Torpdahl, Torpdahl E.M. Nielsen of Applied Microbiology, Volume 103, Issue 1, 1 July 2007, Pages 210–218,...
A genomic cluster of Salmonella Braenderup ST22, a serovar enterica subsp. which causes symptoms gastrointestinal illness, was notified by Danish authorities to the European Centre for Disease Prevention and Control (ECDC) on 3 May 2021. By 6 July 2021, S. outbreak cases (n = 348) had been reported from 12 countries in Union/European Economic Area (EU/EEA) United Kingdom (UK), including 68 hospitalised cases. With support affected EU/EEA countries, partnership with Food Safety Authority...
Abstract Zoonotic Salmonella causes millions of human salmonellosis infections worldwide each year. Information about the source bacteria guides risk managers on control and preventive strategies. Source attribution is effort to quantify number sporadic cases a specific illness sources animal reservoirs. methods for have so far been based traditional wet-lab typing methods. With change whole genome sequencing there need develop new data. Four European datasets collected in Denmark (DK),...
Three large clusters of Salmonella Typhimurium infections in Denmark 2008 and 2009 were defined by multilocus variable number tandem repeat analysis (MLVA). One these proved to be the hereto largest Danish cluster salmonellosis with 1446 cases. Two smaller a total 197 89 cases, respectively, seen concurrently. These shared epidemiological characteristics such as age distribution, geography, time. To investigate possible genetic relationship between strains, further characterized phage...
Salmonella enterica subsp. is one of the leading food-borne pathogens in USA and European countries. Outcome human serotype Typhimurium infections ranges from mild self-limiting diarrhoea to severe that requires hospitalization. Increased knowledge mechanisms are responsible for causing infection especially severity high interest.Strains were selected patients with (n = 9) clinical data allowed us correct known underlying diseases. Additionally, outbreak isolates 3) selected. Strains...
Abstract In Denmark, outbreaks of salmonella with more than 20 cases have become rare. November 2018, an outbreak monophasic Salmonella Typhimurium was detected and investigation initiated the aim identifying source controlling outbreak. Outbreak were defined based on core genome multilocus sequence types. We conducted hypothesis-generating interviews, a matched case-control study, food sampling trace-back investigations. identified 49 distributed across Denmark. univariable analyses...
In 2017, an outbreak of gastroenteritis in England attributed to Salmonella Adjame was detected and investigated. With the introduction whole genome sequencing (WGS) for microbial typing, methods comparing international data require evaluation. A case defined as a person resident with clinical sample from 1 June 2017 27 July whom S . isolated. Cases were interviewed exposures analysed. Backward tracing food provenance undertaken. WGS performed on isolates cases historical compared using...
Salmonella enterica subspecies serovar Typhimurium and its monophasic variant are among the most common serovars associated with human salmonellosis each year. Related infections often due to consumption of contaminated meat pig, cattle poultry origin. In order evaluate novel microbial subtyping methods for source attribution, an approach based on weighted networks was applied 141 210 food animal isolates pigs, broilers, layers, ducks collected in Denmark from 2013 2014. A whole-genome SNP...
To characterize isolates of Salmonella Typhimurium DT41 obtained from infected flocks broiler breeders by multiple-locus variable-number tandem-repeats analysis (MLVA) and compare results with a diverse strain collection Germany United Kingdom Danish patients.A total 102 Salm. phage type were MLVA typed. typing showed 4, 12, 25, 9 8 different alleles at the five loci 9, 5, 6, 10 3, respectively. A dendrogram based on types was constructed, one large group, nine minor groups 29 more unrelated...