A5046463436- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Helicobacter pylori-related gastroenterology studies
- Bacteriophages and microbial interactions
- Veterinary medicine and infectious diseases
- Animal Nutrition and Physiology
- Eosinophilic Esophagitis
- Listeria monocytogenes in Food Safety
- Leptospirosis research and findings
- Aquaculture disease management and microbiota
- Escherichia coli research studies
- Galectins and Cancer Biology
- Vibrio bacteria research studies
- Yersinia bacterium, plague, ectoparasites research
- Tuberculosis Research and Epidemiology
- Gastric Cancer Management and Outcomes
- Food Safety and Hygiene
- Microbial infections and disease research
- Celiac Disease Research and Management
- Gastroesophageal reflux and treatments
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
- Herpesvirus Infections and Treatments
- Peripheral Neuropathies and Disorders
- Trace Elements in Health
University of Surrey
2016-2023
Utrecht University
1991-2016
Animal and Plant Health Agency
2004-2014
Veterinary Medicines Directorate
2003-2014
West Park Hospital
2009
Hannah Research Foundation
2009
University of Aberdeen
2009
The University of Melbourne
2009
University of Glasgow
2009
University of Guelph
2009
The cross sectional study describes the prevalence of infection with Helicobacter pylori as determined by a serodiagnostic assay in over 3000 asymptomatic subjects, two age groups 25-34 years and 55-64 years, from 17 geographically defined populations Europe, North Africa, America, Japan, using common protocol for blood collection serological testing. In all combined, was higher older group (62.4%) than younger (34.9%). There no difference between men women. Subjects education had...
<h3>Abstract</h3> <b>Abstract Objectives</b> : To relate the prevalence of infection with Helicobacter pylori in adults to their living conditions childhood indentify risk factors for infection. <b>Design</b> Prevalence study IgG antibodies H (>10 μg IgG/ml, determined by enzyme linked immunosorbent assay (ELISA)) and reported other socioeconomic childhood. <b>Setting</b> Three factories Stoke on Trent. <b>Subjects</b> 471 male volunteers aged 18 65 years. <b>Main outcome measures</b>...
Abstract To examine the geographic association between Helicobacter pylori infection and gastric cancer, we have assessed prevalence of IgG antibodies to H. in plasma samples taken 1983 from 1882 men, aged 35‐64 years, 46 rural counties People's Republic China. The cancer mortality rates these 1973‐75 varied 3 per 1,000 (cumulative rate, 0‐64 years) 69 1, 000. while proportions population positive for H . (based on an average about 41 men county) 28% 96%. After correction limited number...
Campylobacter jejuni strain 81116 contains two flagellin genes, flaA and flaB. Wild-type (WT) bacteria express only, but flaB can be expressed under certain conditions. We have determined the importance of flagella for colonization avian caecum, which appears to natural environment these bacteria. Mutants in or flaB, both had been inactivated, motility variants, were investigated. Flagella are not a requisite colonization, mutants lacking genes colonized less efficiently than WT....
ABSTRACT The recent development of simple, rapid genotyping techniques for Campylobacter species has enabled investigation the determinative epidemiology these organisms in a variety situations. In this study we have used technique fla typing (PCR-restriction fragment length polymorphism analysis flaA and flaB genes) to identify sources strains contaminating carcasses five campylobacter-positive two campylobacter-negative broiler flocks during abattoir processing. results confirmed that,...
It has recently been shown that the enteropathogen Campylobacter jejuni an N -linked general p rotein gl ycosylation pathway (Pgl) modifies many of organism's proteins. To determine role glycosylation in C , authors studied pglH gene, which shows high similarity to a family sugar transferases. mutants were constructed strains 81116 and 11168H. Both be deficient their ability glycosylate number C. proteins, but lipooligosaccharide capsule unaffected. The had significantly reduced adhere...
The genome sequence of the enteric bacterial pathogen Campylobacter jejuni NCTC 11168 (11168-GS) was published in 2000, providing a valuable resource for identification C. jejuni-specific colonization and virulence factors. Surprisingly, 11168-GS clone subsequently found to colonize 1-day-old chicks following oral challenge very poorly compared other strains. In contrast, we have that original clinical isolate from which derived, 11168-O, is an excellent colonizer chicks. Other marked...
The combination of virulence gene and antimicrobial resistance typing using DNA arrays is a recently developed genomics-based approach to bacterial molecular epidemiology. We have now applied this technology 523 Salmonella enterica subsp. strains collected from various host sources public health veterinary institutes across nine European countries. strain set included the five predominant serovars isolated in Europe (Enteritidis, Typhimurium, Infantis, Virchow, Hadar). Initially, these were...
SUMMARY An abattoir survey was undertaken to determine the prevalence of foodborne zoonotic organisms colonizing cattle, sheep and pigs at slaughter in Great Britain. The study ran for 12 months from January 2003, involved 93 abattoirs collected 7703 intestinal samples. design similar two previous surveys 1999–2000 allowing comparisons. Samples were examined VTEC O157, Salmonella , thermophilic Campylobacter Yersinia enterocolitica . O157 faecal carriage 4·7% 0·7% 0·3% pigs. A significant...
ABSTRACT Multilocus sequence typing (MLST) has been applied to 266 Campylobacter jejuni isolates, mainly from veterinary sources, including cattle, sheep, poultry, pigs, pets, and the environment, as well isolates human cases of campylobacteriosis. The populations overlap, suggesting that most sources should be considered reservoirs pathogenic campylobacters. There were some associations between source type complex, indicating host or adaptation may exist. pig formed a distinct group by MLST...
ABSTRACT Poultry isolates of Campylobacter jejuni derived from a survey meat processing batches were genotyped by pulsed-field gel electrophoresis (PFGE) chromosomal DNA to establish the clonal relationships between single-colony isolates. In majority studied, one or two genotype patterns predominated. However, in batch (batch A), 21 gave 14 different PFGE genotypes. The banding obtained with Sma I sufficiently distinguish genotypes, although also produced many common bands. question whether...
Although S-layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S-layer function continues to elude us. In a few instances, have been shown be virulence factors pathogens (e.g. Campylobacter fetus ssp. Aeromonas salmonicida), protective against Bdellovibrio, depository for surface-exposed enzymes Bacillus stearothermophilus), shape-determining agents Thermoproteus tenax) nucleation fine-grain mineral development Synechococcus GL 24). Yet, the...
ABSTRACT Lipopolysaccharide (LPS) is one of the main virulence factors gram-negative bacteria. The LPS from Campylobacter spp. has endotoxic properties and been shown to play a role in adhesion. We previously cloned gene cluster ( wla ) which involved synthesis jejuni 81116 molecule. Sequence alignment first this indicated similarity with galE genes. These genes encode UDP-glucose 4-epimerase, catalyzes interconversion UDP-galactose UDP-glucose. A Salmonella mutant was transformed C. ....
SUMMARY The biochemical and biological properties of the flagella Campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): non-motile variant (SF-1) an aflagellate (SF-2). Phenotypic analysis strains amino acid isolated suggest that differed wild-type strain only in absence and/or motility. aflagellato poorly colonized gastrointestinal tract infant mice but as successfully strain. 35 S-labelled organisms were used to...
Histological examination of the stomachs Rhesus monkeys at autopsy showed chronic gastritis in a high proportion all ages. Lesions consisted mild to heavy infiltration lamina propria by lymphocytes, plasma cells, and histiocytes. The antrum was most consistently affected, but lesions were also present fundus pylorus. Gastric Campylobacter-like organisms (GCLO) apparently identical human C pylori cultured and/or detected immunohistologically several animals. Electron microscopy spiral...
Campylobacter jejuni strain M1 (laboratory designation 99/308) is a rarely documented case of direct transmission C. from chicken to person, resulting in enteritis. We have sequenced the genome M1, and compared this 12 other genomes currently publicly available. Compared these, closest 81116. Based on 13 sequences, we identified pan-genome, as well core genome, auxiliary genes, genes unique between strains The pan-genome contains 2,427 gene families, whilst comprised 1,295 or about...
ABSTRACT Campylobacter jejuni colonizes the intestines of domestic and wild animals is a common cause human diarrheal disease. We identified two-component regulatory system, designated RacR-RacS (reduced ability to colonize) that involved in temperature-dependent signalling pathway. A mutation response regulator gene racR reduced organism’s colonize chicken intestinal tract resulted changes its protein profile growth characteristics.