A5078415408- Animal Behavior and Welfare Studies
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Plant and fungal interactions
- Viral gastroenteritis research and epidemiology
- Helminth infection and control
- Rabies epidemiology and control
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Reproductive Physiology in Livestock
- Nail Diseases and Treatments
- Enzyme Production and Characterization
- Studies on Chitinases and Chitosanases
- Dermatology and Skin Diseases
- Herpesvirus Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- Ruminant Nutrition and Digestive Physiology
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Corrosion Behavior and Inhibition
- Escherichia coli research studies
- Health, psychology, and well-being
University of Bristol
2010-2021
At Bristol
2017
Institute of Infection and Immunity
2009-2010
Département Santé Animale
2006
Royal Veterinary College
1994-2002
Although S-layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S-layer function continues to elude us. In a few instances, have been shown be virulence factors pathogens (e.g. Campylobacter fetus ssp. Aeromonas salmonicida), protective against Bdellovibrio, depository for surface-exposed enzymes Bacillus stearothermophilus), shape-determining agents Thermoproteus tenax) nucleation fine-grain mineral development Synechococcus GL 24). Yet, the...
Footrot is an infectious bacterial disease of sheep that causes lameness. The causal agent Dichelobacter nodosus. There debate regarding the role Fusobacterium necrophorum in initiation. This research used observational longitudinal study footrot, together with quantitative PCR (qPCR) load D. nodosus and F. necrophorum, to elucidate roles each species development disease. All feet 18 a priori selected were monitored for five weeks assessing severity (healthy, interdigital dermatitis (ID)...
A postal survey of the techniques being used for treatment and control footrot in sheep flocks between November 1999 October 2000 was conducted England Wales 2000. Of 392 questionnaires circulated, 251 (64 per cent) were returned, 209 these usable. Negative binomial regression analysis indicated that isolation bought‐in sheep, separation individual diseased with parenteral antibiotics, foot trimming topical sprays associated a significantly lower prevalence flock. In contrast, ewe which...
Although S-layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S-layer function continues to elude us. In a few instances, have been shown be virulence factors pathogens (e.g. Campylobacter fetus ssp. Aeromonas salmonicida), protective against Bdellovibrio, depository for surface-exposed enzymes Bacillus stearothermophilus), shape-determining agents Thermoproteus tenax) nucleation fine-grain mineral development Synechococcus GL 24). Yet, the...
SUMMARY Enrichment culture is often used to isolate Campylobacter . This study compared isolation of spp. from 119 broiler chicken environments two farms, using Preston and modified Exeter (mExeter) Bolton (mBolton) enrichments. mExeter was significantly more effective in isolating the environmental samples ( P <0·001) mBolton <0·04) broths but there no significant difference between latter methods >0·05). broth type did not affect faecal or soil litter samples. C. jejuni isolated...
Footrot, including interdigital dermatitis, is caused by Dichelobacter nodosus cause the majority of lameness in sheep UK. Lame often have overgrown hoof horn but recent evidence has indicated that trimming increases recovery time, and routine foot flock does not reduce prevalence or incidence lameness. The objectives this study were to investigate temporal associations between length, footrot climate. Fifty multiparous ewes monitored for 10 months. On eight occasions lesions body condition...
ABSTRACT The role of the surface (S)-layer proteins Campylobacter fetus subsp. has been investigated using an ovine model abortion. Wild-type strain 23D induced abortion in up to 90% pregnant ewes challenged subcutaneously. Isolates recovered from both dams and fetuses expressed S-layer with variable molecular masses. spontaneous S-layer-negative variant, 23B, neither colonized nor caused abortions ewes. A series isogenic sapA recA mutants, derived 23D, also were this model. mutant (501 [ +...
ABSTRACT A collection of Campylobacter fetus strains, including both C. subsp. and venerealis , were phenotypically identified to the subspecies level genotypically typed by PCR amplified fragment length polymorphism (AFLP) analysis. Phenotypic determination methods unreliable. Genotyping strains AFLP showed a clear discrimination between two subspecies.
Surface layer proteins (SLPs) are essential for induction of abortion by Campylobacter fetus subsp. in experimentally challenged ewes. These encoded multiple sap genes and vary size antigenicity. The role SLP antigenic variation during experimental ovine infection was investigated. Following subcutaneous challenge, the SLPs were highly antigenic, antibodies detected serum, milk, bile, urine. Fecal anti-SLP only animals orally. Ewes with wild-type strain 23D variable developed detectable...
Analysis of bacterial populations in situ provides insights into pathogen population dynamics and potential reservoirs for disease. Here we report a culture-independent study ovine footrot (FR); debilitating disease that has significant economic impact on sheep farming worldwide. Disease begins as an interdigital dermatitis (ID), which may then progress to separation the hoof horn from underlying epidermis causing severe (SFR). Dichelobacter nodosus is causative agent FR, however, role...
Published research relevant to the UK dairy goat industry is scarce. Current practices and concerns within must be better understood if have optimal value. A postal survey was conducted of farmer membership Milking Goat Association as a first step in addressing gaps knowledge. Questions were asked about husbandry practices, observations their goats priorities for further research. Seventy‐three per cent members responded, representing 38 commercial farms 53 population England Wales. Findings...
As part of an investigation into improving the treatment and control lameness in sheep flocks England Wales, a postal survey was conducted November 2000. Farmers were asked to estimate prevalence footrot interdigital dermatitis their flocks. In ewes remained relatively stable throughout year, but there large increase lambs during late spring early summer. Logistic regression models used assess statistically significant risk factors associated with prevalences condition 5 per cent or more 10...
Five UK goat farms with high levels of lameness (prevalence 14-67 per cent) were investigated. On two (farms 1 and 2), the animals presented typical footrot lesions. The remaining three 3, 4 5) infected lesions on foot that did not resemble footrot. These observed to start from white line or sole but interdigital space was rarely affected. Swabs processed by PCR assess presence Dichelobacter nodosus specific treponeme groups (group 1: Treponema medium/Treponema vincentii-like, group 2:...
In 1999, a study was initiated to improve the treatment and control of footrot interdigital dermatitis in sheep flocks England Wales. November 2000, retrospective cross‐sectional conducted which 392 farmers were asked estimate prevalence their flock previous 12 months, whether they considered these diseases be problem, how treated controlled them opinion on success measures that used; 209 provided usable responses. The tended more concerned as increased; 91 per cent with less than 5 it small...
Dichelobacter nodosus (D. nodosus) is the essential causative agent of footrot in sheep. The current study investigated when D. was detectable on newborn lambs and possible routes transmission. Specific qPCR used to detect quantify load foot swabs at birth 5-13 h post-partum, their mothers post-partum; samples bedding, pasture, soil faeces. not detected feet swabbed birth, but after once they had stood bedding containing naturally occurring nodosus. Multiple genotypes identified by cloning...